FastQCFastQC Report
Wed 21 Jun 2023
sRNA-POC-48-S1-TP2.flexbar_trim.20230621_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-48-S1-TP2.flexbar_trim.20230621_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16574535
Sequences flagged as poor quality0
Sequence length18-35
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCA321554119.400489968496853No Hit
GAGAATTCTACCACTGAACCACCAGTGC10059606.069310541743705No Hit
ATAGACACTCAGACAGACATGCTCCTGGGAGAACC5777293.4856422819705046No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT4362652.632140207855002Illumina RNA PCR Primer (100% over 35bp)
GAGAATTCTACCACTGAACCACAGTGC2409781.4539050416799024No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGC1859891.122137061462056No Hit
AGAATTCTACCACTGAACCACCAGTGC1696201.0233771264171212No Hit
CGCTTCAAGTCTCAGCCCGAG1437550.8673244830096288No Hit
GACCGCGACCTCGGAAAGGG1198000.7227955414737125No Hit
GACCGCGACCTCGGAAAGGGA1182500.7134438462376169No Hit
GACCGCGACCTCGGAAAG1150950.6944086214183384No Hit
TGCTTCAAGTCTCAGCCCGAG972960.5870209933491347No Hit
GACCGCGACCTCGGAAAGGGAG965710.582646813319348No Hit
GGCCAGTCCGGGCCGGATCC882010.5321476590444317No Hit
AACCGCTTCAAGTCTCAGCCCGAG836380.5046174749397192No Hit
GGCCAGTCCGGGCCGGATCCAAC790710.4770631574279459No Hit
TGACAGGGTCGAGTTCGACTATCTTGGTCG752010.4537140860965331No Hit
AAACTAACCAGCCTAGACTTGA720920.43495639545845477No Hit
TCCACTTCAGAGCAAAGATTTTCA704550.4250797986187848No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGC679010.4096706182104053No Hit
ATCCACTTCAGAGCAAAGATTTTCA600260.3621579730592744No Hit
ACAAGTTCGGATCTACGGGT577410.34837176427573985No Hit
GACCGCGACCTCGGAAAGG549720.33166541323783744No Hit
GCCAGTCCGGGCCGGATCC524860.31666650074949315No Hit
AACCGCTTCAAGTCTCAGCCCGAGA509000.3070976048498495No Hit
CGCTTCAAGTCTCAGCCCGAGA500740.3021140562917753No Hit
GTAGACACTCAGACAGACATGCTCCTGGGAGAACC473460.285655072676247No Hit
GGCGAGAATTCTACCACTGAACCACAGTGC455750.27497000670003713No Hit
TGCCAGTCCGGGCCGGATCC434290.2620224338118686No Hit
CCACAAGTTCGGATCTACGGGT431250.26018829487524087No Hit
TGCCAGTCCGGGCCGGATCCAAC406360.24517128233160088No Hit
ACTCGTTCTCAAAGTAGTTCAGCAGAA383070.23111960607039655No Hit
AGAATTCTACCACTGAACCACAGTGC380630.22964746823968216No Hit
CTCCACTTCAGAGCAAAGATTTTCA369630.22301078129793686No Hit
CCACTTCAGAGCAAAGATTTTCA340940.20570109508351214No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGC340230.2052727271081813No Hit
TGCTTCAAGTCTCAGCCCGAGA338460.20420482384573682No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG328640.19828007241228787No Hit
GAGAATTCTACCACTGAACCACAAGTGC314800.1899299135692193No Hit
GGAATTCTACCACTGAACCACCAGTGC301750.1820563895156033No Hit
TCCCACTTCAGAGCAAAGATTTTCA269920.16285223084689857No Hit
TTCCACCTCAGAGCAAAGATTTTCA262620.1584478840582858No Hit
GCGAGAATTCTACCACTGAACCACCAGTGC260000.1568671458957974No Hit
GCTTCAAGTCTCAGCCCGAG250610.151201828588253No Hit
GCCTCGACCACGGCCAGTCCG228740.13800688827771035No Hit
TACCGCGACCTCGGAAAGGGA227080.13700535188468332No Hit
TACCGCGACCTCGGAAAGGG224750.1355995809233864No Hit
TACCGCGACCTCGGAAAG223680.1349540122845075No Hit
CGGAAACGTTCGAGTTGGCTATCGGTAGTG223560.13488161206332486No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGC223390.13477904508331606No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGAT221220.13346980775026268No Hit
TTCCACTTCAGAGCAAAGATTTTCG202730.1223141403363654No Hit
AGCCAGTCCGGGCCGGATCC202060.12190990576809546No Hit
GAGAATTCTACCACTGAACCACCAGTGT199190.12017833381147645No Hit
AGTCCGGGCCGGATCCAAC198540.11978616594673698No Hit
TCATGCTCTACCGACTGAGCTAGCCGGGC196770.1187182626842925No Hit
TTCCCGGTCCGACCGCGACCTCGGAAAG196500.11855536218663147No Hit
TACCGCGACCTCGGAAAGGGAG190750.11508618492162828No Hit
GGCCAGTCCGGGCCGGATCCA186670.11262457740141729No Hit
GTCGAGTTCGACTATCTTGGTC184040.11103780588716365No Hit
TGCGAGAATTCTACCACTGAACCACAGTGC182010.10981303547882339No Hit
GCCAGAATTGCCTGAAGCCGTGCAGGAA180080.10864859858813534No Hit
TGAATTCTACCACTGAACCACCAGTGC176290.10636195826911583No Hit
CCAGAATTGCCTGAAGCCGTGCAGGAA175160.10568018951964564No Hit
AGCCAGTCCGGGCCGGATCCAAC174130.10505875428782768No Hit
TAGAATTCTACCACTGAACCACCAGTGC172990.10437095218659227No Hit
GACAGGGTCGAGTTCGACTATCTTGGTCG171420.10342371595945227No Hit
AGCCGGGCTGTAACAGTCCGGAGCCGAGACTCCAG167360.10097417514277174No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)