FastQCFastQC Report
Wed 21 Jun 2023
sRNA-POC-48-S1-TP2.flexbar_trim.20230621_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-48-S1-TP2.flexbar_trim.20230621_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16574535
Sequences flagged as poor quality0
Sequence length18-35
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA323155919.497132197072194No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC10202256.15537630467461No Hit
AACTTTTGACGGTGGATCTCTAGGCTCACGCATCG5506213.3220901823188402No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACACTGAT4270782.576711805187898TruSeq Adapter, Index 25 (100% over 35bp)
GCACTGTGGTTCAGTGGTAGAATTCTC2439241.4716792959802492No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC1908091.151217816970431No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT1711291.0324814542308427No Hit
CTCGGGCTGAGACTTGAAGCG1451980.8760306096068456No Hit
CCCTTTCCGAGGTCGCGGTC1223760.7383374556209269No Hit
TCCCTTTCCGAGGTCGCGGTC1208340.729034027198953No Hit
CTTTCCGAGGTCGCGGTC1176220.7096549013290568No Hit
CTCCCTTTCCGAGGTCGCGGTC989050.5967286563393784No Hit
CTCGGGCTGAGACTTGAAGCA974450.587919962762153No Hit
GGATCCGGCCCGGACTGGCC893860.539297180886221No Hit
CTCGGGCTGAGACTTGAAGCGGTT838800.5060775460669032No Hit
GTTGGATCCGGCCCGGACTGGCC801240.4834162768367257No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCA765710.4619797780148885No Hit
CAACTTTTGACGGTGGATCTCTAGGCTCACGCATC754280.4550836569472387No Hit
TCAAGTCTAGGCTGGTTAGTTT723670.43661556719389105No Hit
TGAAAATCTTTGCTCTGAAGTGGA722680.436018265369134No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA677310.4086449484103174No Hit
TGAAAATCTTTGCTCTGAAGTGGAT584870.3528726446925962No Hit
ACCCGTAGATCCGAACTTGT584100.352408076606674No Hit
CCTTTCCGAGGTCGCGGTC560020.3378797655560171No Hit
GGATCCGGCCCGGACTGGC539710.32562602812084923No Hit
TCTCGGGCTGAGACTTGAAGCGGTT510550.30803277437345905No Hit
TCTCGGGCTGAGACTTGAAGCG505460.30496179832496056No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC466500.2814558598476518No Hit
ACCCGTAGATCCGAACTTGTGG449620.2712715620679555No Hit
GGATCCGGCCCGGACTGGCA434070.2618897000730337No Hit
GTTGGATCCGGCCCGGACTGGCA406290.2451290488692443No Hit
TTCTGCTGAACTACTTTGAGAACGAGT385740.2327305109917111No Hit
GCACTGTGGTTCAGTGGTAGAATTCT384730.23212114246342355No Hit
TGAAAATCTTTGCTCTGAAGTGGAG372740.22488715369692122No Hit
TGAAAATCTTTGCTCTGAAGTGG344050.20757746748249647No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT340520.20544769430937276No Hit
TCTCGGGCTGAGACTTGAAGCA338530.2042470573080934No Hit
GCACTTGTGGTTCAGTGGTAGAATTCTC318590.1922165538882388No Hit
GCACTGGTGGTTCAGTGGTAGAATTCC308270.18599013486652868No Hit
TGAAAATCTTTGCTCTGAAGTGGGA272470.16439073554703043No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC269330.16249626309275042No Hit
TGAAAATCTTTGCTCTGAGGTGGAA265670.1602880563466788No Hit
CTCGGGCTGAGACTTGAAGC260300.1570481464487541No Hit
CGGACTGGCCGTGGTCGAGGC232750.14042626233556477No Hit
CACTACCGATAGCCAACTCGAACGTTTCCG230670.1391713251683984No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCT225480.13604001560224768No Hit
TCCCTTTCCGAGGTCGCGGTA224760.1356056142751516No Hit
CCCTTTCCGAGGTCGCGGTA222520.13425414347974166No Hit
CTTTCCGAGGTCGCGGTA221320.1335301412679149No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTA205400.12392504525767993No Hit
ACACTGGTGGTTCAGTGGTAGAATTCTC203790.12295367562347903No Hit
GGATCCGGCCCGGACTGGCT203320.12267010809051354No Hit
CGAAAATCTTTGCTCTGAAGTGGAA201680.12168063840101698No Hit
GTTGGATCCGGCCCGGACT200720.12110143663155559No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA197100.11891736329254486No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA194080.1170952910594475No Hit
CTCCCTTTCCGAGGTCGCGGTA189460.11430788254391451No Hit
GACCAAGATAGTCGAACTCGAC189450.1143018491921493No Hit
TGGATCCGGCCCGGACTGGCC188350.11363818049797475No Hit
TTCCTGCACGGCTTCAGGCAATTCTGGC184560.11135154017895524No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA181620.10957773475997969No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC178600.10775566252688236No Hit
TTCCTGCACGGCTTCAGGCAATTCTGG176530.10650675871148119No Hit
GCACTGGTGGTTCAGTGGTAGAATTCA176300.10636799162088108No Hit
GTTGGATCCGGCCCGGACTGGCT174890.10551728902198462No Hit
ACCCGTAGATCCGAACTTGTGA170860.10308584826059977No Hit
CTGGAGTCTCGGCTCCGGACTGTTACAGCCCGGCT168830.10186107785225951No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)