FastQCFastQC Report
Wed 21 Jun 2023
sRNA-POC-47-S1-TP2.flexbar_trim.20230621_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-47-S1-TP2.flexbar_trim.20230621_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15063975
Sequences flagged as poor quality0
Sequence length18-35
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCA220674014.649121496816079No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT6970374.627178417383194Illumina RNA PCR Primer (100% over 35bp)
ATAGACACTCAGACAGACATGCTCCTGGGAGAACC6523194.330324499343632No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGC2907871.930347069747527No Hit
TTCCACTTCAGAGCAAAGATCTTCA1392330.9242779545239552No Hit
GAGAATTCTACCACTGAACCACCAGTGC1211110.8039777017686234No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGC1030240.6839097914063187No Hit
ACAAGTTCGGATCTACGGGT989830.6570842025428215No Hit
AAACTAACCAGCCTAGACTTGA945690.6277825076050644No Hit
GGCGAGAATTCTACCACTGAACCACAGTGC892180.5922606748882682No Hit
TGACAGGGTCGAGTTCGACTATCTTGGTCG891710.5919486722462033No Hit
AACCGCTTCAAGTCTCAGCCCGAG817710.5428248520061936No Hit
GACCGCGACCTCGGAAAGGG729420.48421482377659286No Hit
GACCGCGACCTCGGAAAG720370.4782071133283214No Hit
GACCGCGACCTCGGAAAGGGAG586070.3890540179467903No Hit
GTAGACACTCAGACAGACATGCTCCTGGGAGAACC572180.3798333441206587No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGC551480.366091951161629No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG534110.3545611301133997No Hit
CCACAAGTTCGGATCTACGGGT510640.33898091307241285No Hit
ATCCACTTCAGAGCAAAGATTTTCA500570.3322960905073196No Hit
AGAATTCTACCACTGAACCACCAGTGC498900.33118748537487613No Hit
GCCAGAATTGCCTGAAGCCGTGCAGGAA490330.32549841592275613No Hit
CGCTTCAAGTCTCAGCCCGAG459520.3050456469822872No Hit
TCCACTTCAGAGCAAAGATTTTCA450720.2992038953861779No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGC432070.2868233650148782No Hit
GAGAATTCTACCACTGAACCACAGTGC384330.25513186260598547No Hit
CCAGAATTGCCTGAAGCCGTGCAGGAA369960.24559254778370251No Hit
TGCTTCAAGTCTCAGCCCGAG368030.24431134544501037No Hit
GCCAGTCCGGGCCGGATCC341120.22644753459827172No Hit
TGCGAGAATTCTACCACTGAACCACAGTGC336550.22341380678074677No Hit
GACGTACTGTCGTCAGCGTTCAAATATGGAAA321950.2137218098144746No Hit
CTCCACTTCAGAGCAAAGATTTTCA314500.2087762360200412No Hit
GCAAGTTCGGATCTACGGGT274370.18213652107096567No Hit
TTCCACCTCAGAGCAAAGATTTTCA257080.17065880685542828No Hit
AACCGCTTCAAGTCTCAGCCCGAGA241760.16048884839492897No Hit
TCACAAGTTCGGATCTACGGGT235690.15645936746443087No Hit
TTTTTTTGGTCTTTCACCTACAGCATTAATA232630.1544280311139656No Hit
CCACTTCAGAGCAAAGATTTTCA228360.1515934539190353No Hit
TGGCGAGAATTCTACCACTGAACCACCAGTGC223970.14867921647506716No Hit
TCAGAATTGCCTGAAGCCGTGCAGGAA223610.14844023572795362No Hit
GGCCAGTCCGGGCCGGATCC223520.14838049054117522No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGAT221150.14680720062267763No Hit
GACCGCTTCAAGTCTCAGCCCGAG215510.1430631689178985No Hit
GAACTAACCAGCCTAGACTTGA210030.1394253508785032No Hit
GTACTGTCGTCAGCGTTCAAATATGGAAA209600.1391399016527842No Hit
TCCCACTTCAGAGCAAAGATTTTCA207550.1377790390650542No Hit
GTCGTCAGCGTTCAAATATGGAAACAGA195130.12953420328963636No Hit
AAATCCATAGAATCTCAGCAGTT192880.1280405736201766No Hit
TGTACTGTCGTCAGCGTTCAAATATGGAAA191280.12697843696633856No Hit
TGCGTGAGCCTAGAGATCCACCGTCAAAAGTT188750.12529893338245718No Hit
GACCCGAGGGTCACAGCTG184950.12277635882959179No Hit
AGCGAGAATTCTACCACTGAACCACAGTGC174280.11569323501930931No Hit
ACTCGTTCTCAAAGTAGTTCAGCAGAA173120.11492318594527673No Hit
GACGTACTGTCGTCAGCGTTCAATATGGAAA173020.11485680240441185No Hit
TTCCCGGTCCGACCGCGACCTCGGAAAG165300.10973199304964328No Hit
AGATCCACCGTCAAAAGTT164720.10934696851262699No Hit
AGAATTCTACCACTGAACCACAGTGC157600.10462046040304766No Hit
ATTGGTGAGATGGTGGGACTGA155210.10303389377637709No Hit
TGTGAGATAACCCTCAGCCGTCCGGACCTC152410.10117515463216051No Hit
GACCGCGACCTCGGAAAGGGA151540.10059761782663607No Hit
CCTTCCTTAGGGAGTTACACAA150780.10009310291606299No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)