FastQCFastQC Report
Wed 21 Jun 2023
sRNA-POC-47-S1-TP2.flexbar_trim.20230621_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-47-S1-TP2.flexbar_trim.20230621_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15063975
Sequences flagged as poor quality0
Sequence length18-35
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA222851114.793645103633004No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGGTAGC6445494.278744488091622TruSeq Adapter, Index 22 (96% over 33bp)
AACTTTTGACGGTGGATCTCTAGGCTCACGCATCG6289304.175060035614769No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC2998601.9905768563742308No Hit
TGAAGATCTTTGCTCTGAAGTGGAA1404370.9322705328440867No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC1236520.820845759502389No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA1027100.6818253482231615No Hit
ACCCGTAGATCCGAACTTGT1015120.6738726000275492No Hit
TCAAGTCTAGGCTGGTTAGTTT955770.6344739685242441No Hit
CAACTTTTGACGGTGGATCTCTAGGCTCACGCATC933650.6197899292849331No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC919140.6101576775054394No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCA914520.607090757917482No Hit
CTCGGGCTGAGACTTGAAGCGGTT827370.5492375020537408No Hit
CCCTTTCCGAGGTCGCGGTC751430.49882584112095246No Hit
CTTTCCGAGGTCGCGGTC743220.493375752415946No Hit
CTCCCTTTCCGAGGTCGCGGTC605830.4021714056216902No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT558440.3707122456058245No Hit
ACCCGTAGATCCGAACTTGTGG537890.3570704279580921No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT509270.338071458562564No Hit
TTCCTGCACGGCTTCAGGCAATTCTGGC505470.3355488840096987No Hit
TGAAAATCTTTGCTCTGAAGTGGAT493110.3273438783587997No Hit
CTCGGGCTGAGACTTGAAGCG467750.3105090123954667No Hit
TGAAAATCTTTGCTCTGAAGTGGA459340.30492615660873046No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCT441770.2932625684787714No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC392080.26027658702301354No Hit
TTCCTGCACGGCTTCAGGCAATTCTGG375340.24916398228223294No Hit
CTCGGGCTGAGACTTGAAGCA368820.24483577541784288No Hit
GGATCCGGCCCGGACTGGC351690.2334642748676893No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA335090.22244460708411956No Hit
TTTCCATATTTGAACGCTGACGACAGTACGTC331680.22018092834062722No Hit
TGAAAATCTTTGCTCTGAAGTGGAG316980.21042254783349015No Hit
ACCCGTAGATCCGAACTTGC282080.18725469207164774No Hit
TGAAAATCTTTGCTCTGAGGTGGAA260810.17313491292968822No Hit
ACCCGTAGATCCGAACTTGTGA244710.16244716285044286No Hit
TCTCGGGCTGAGACTTGAAGCGGTT244600.1623741409554915No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA235150.15610089634376054No Hit
TGAAAATCTTTGCTCTGAAGTGG231750.15384385595435468No Hit
GGATCCGGCCCGGACTGGCC228660.15179260454162996No Hit
TTCCTGCACGGCTTCAGGCAATTCTGA224930.14931649846737No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCA224710.14917045467746728No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTA222690.14782950715199675No Hit
CTCGGGCTGAGACTTGAAGCGGTC218980.14536667778590975No Hit
TTTCCATATTTGAACGCTGACGACAGTAC217700.14451696846283932No Hit
TCAAGTCTAGGCTGGTTAGTTC213710.1418682651823307No Hit
TGAAAATCTTTGCTCTGAAGTGGGA209570.13911998659052474No Hit
TCTGTTTCCATATTTGAACGCTGACGAC201120.13351057738744254No Hit
CAGCTGTGACCCTCGGGTC194430.12906951850358223No Hit
TTTCCATATTTGAACGCTGACGACAGTACA193880.12870440902882538No Hit
AACTGCTGAGATTCTATGGATTT193870.1286977706747389No Hit
AACTTTTGACGGTGGATCTCTAGGCTCACGCA190860.12669962609470609No Hit
TTTCCATATTGAACGCTGACGACAGTACGTC178120.11824236298852062No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT176440.11712711950199067No Hit
TTCTGCTGAACTACTTTGAGAACGAGT175990.11682839356809874No Hit
AACTTTTGACGGTGGATCT168900.11212180052077889No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA166420.1104754887073299No Hit
GATACGATCCACTGAGATTCATCCTGTGTTTTTCG164290.10906151928690799No Hit
GCACTGTGGTTCAGTGGTAGAATTCT160700.10667835016985888No Hit
TCAGTCCCACCATCTCACCAAT158750.10538387112299377No Hit
TCCCTTTCCGAGGTCGCGGTC156080.10361143058190152No Hit
GAGGTCCGGACGGCTGAGGGTTATCTCACA153760.10207133243383636No Hit
TTGTGTAACTCCCTAAGGAAGG152910.1015070723364849No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCC152360.10114196286172805No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)