FastQCFastQC Report
Wed 21 Jun 2023
sRNA-POR-82-S1-TP2.flexbar_trim.20230621_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POR-82-S1-TP2.flexbar_trim.20230621_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17260661
Sequences flagged as poor quality0
Sequence length18-35
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGAGAATTCTACCACTGAACCACCAGTGC5227983.0288411318662707No Hit
TTCCACTTCAGAGCAAAGATTTTCA3975062.30295931308772No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT3352091.942040342487463Illumina RNA PCR Primer (100% over 35bp)
AGCGAGAATTCTACCACTGAACCACCAGTGC2659771.5409433045466798No Hit
AGACAACTACTCACGCTTAAGCACAC2633501.5257237251806288No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGAT2560711.483552686655511No Hit
AGCAGACAACTACTCACGCTTAAGCACAC1903821.1029820932118417No Hit
AAGGATGATAACCCTCGACCGTCCGGACCTC1244750.7211485122151463No Hit
AGTAGATAACCCTCCCCAAGTCCGGACCTC1140290.6606293930458399No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGC959080.5556450010807813No Hit
GGCGAGAATTCTACCACTGAACCACAGTGC888540.5147775047548874No Hit
AAAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATA873660.5061567456773527No Hit
AAACTAACCAGCCTAGACCTGA795690.46098466333357685No Hit
ACAAGTTCGGATCTACGGGA792230.4589801051072146No Hit
TCCGACACTCAGACAGACATGCTCCTGGGAGAACC714090.4137095329083863No Hit
AAGCCTACAACACGGGGTATTCCCAGGCGGTCACC704140.4079449796273735No Hit
GTGGATAACCCTCCTCCGTCCGGACCTC687870.3985189211467626No Hit
AATGAGGTCAATGAAGGCGGGGAGGGGCGTACCGA620370.35941265517004245No Hit
AGAATCGTGGATAACCCTCCTCCGTCCGGACCTC554680.321355016473587No Hit
AGCGAGAATTCTACCACTGAACCACAGTGC452770.2623132451300677No Hit
ACGGGATCGGTCACGGTAA436510.25289298017034223No Hit
ACACATCCACTAGCGGTAGGTTGCCAG427410.24762087616459183No Hit
ACACTCTTCTTGGTGCAGGAGTCGACAGCT424560.24596972271224143No Hit
GGACAACTACTCACGCTTAAGCACAC397790.23046046730191852No Hit
AGCCGACAGTACCCTAAGCACTTCCCAACTAGTCC395350.22904684820587115No Hit
GCTCTTCTAGGTGCAGGAGTCGACAGCT393150.22777227361107433No Hit
AAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATAG391670.2269148325200292No Hit
AATCGTGGATAACCCTCCTCCGTCCGGACCTC389850.22586041171887913No Hit
AAACACAGCAGACAACTACTCACGCTTAAGCACAC381530.22104020234219304No Hit
TTCCACTTCAGAGAAAAGATTTTCA381340.22093012544536966No Hit
GCGAGAATTCTACCACTGAACCACCAGTGC379440.21982935647713608No Hit
ATAACCCTCCTCCGTCCGGACCTC356130.2063246592931754No Hit
GCAAATCTTAGAACAAAGGCTTAATCTCAGAAGAT332990.19291845196426718No Hit
GGCAGACAACTACTCACGCTTAAGCACAC328680.19042144446264253No Hit
AGTAGTAGATAACCCTCCCCAAGTCCGGACCTC328550.19034612869113182No Hit
AGGATGATAACCCTCGACCGTCCGGACCTC301290.1745529907574223No Hit
GAATCGTGGATAACCCTCCTCCGTCCGGACCTC297430.17231669169564248No Hit
AGCAGAGATTGGTGAGCTGAGA296180.17159250158496248No Hit
AGTAGATAACCCTCCCCAAATCCGGACCTC278990.16163343918289108No Hit
ATCAGAGGACAATTTCACATTCCTCACTAACAGTC278470.16133217609684822No Hit
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGC262700.15219579366050928No Hit
ATGAGGTCAAGTTAGTGGAAGAGGGGTG254810.14762470568189712No Hit
GCTGAGTTCATCAACAGAATATTTAAACAA251330.145608560413764No Hit
GATAACCCTCAACCGTCCGGACCTC243610.14113596229020428No Hit
GTCTCATGCTCTACCGACTGAGCTAGCCGGGC228100.13215021139688682No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGC222820.1290912323693745No Hit
AAGAATGGCGCAAGAATCCAAAGTGGTCA216410.12537758548180747No Hit
ATCATCAGAGGACAATTTCACATTCCTCACTAACA213550.1237206385085716No Hit
AGTGAGGTCTAAGAGCGGGGAGGGGGGAGATCAAC210100.1217218738030948No Hit
AATGAGGTCAAGTTAGTGGAAGAGGGGTG208780.12095712904621671No Hit
ATAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATA199950.11584145010437316No Hit
AATGAGGTCAAGTTAGTGGAAGAGGGGTGATTCAT198470.11498400901332805No Hit
CGTGGATAACCCTCCTCCGTCCGGACCTC191550.11097489256060357No Hit
CGCTCTTCTAGGTGCAGGAGTCGACAGCT190830.11055775905685188No Hit
ACGAGAATTCTACCACTGAACCACCAGTGC190800.11054037849419557No Hit
AAAAGAGGTCAAGTATTTGGAGGAGGGGTGTCTAT174840.1012939191610333No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)