FastQCFastQC Report
Wed 21 Jun 2023
sRNA-POR-82-S1-TP2.flexbar_trim.20230621_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POR-82-S1-TP2.flexbar_trim.20230621_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17260661
Sequences flagged as poor quality0
Sequence length18-35
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC5303593.072645943281083No Hit
TGAAAATCTTTGCTCTGAAGTGGAA4005382.3205252684123745No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGAGTGG3099011.7954179159187473TruSeq Adapter, Index 7 (96% over 32bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT2650251.535427872663741No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCT2631741.5247040655047914No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCT1902771.1023737735188703No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTT1242820.7200303626842564No Hit
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCT1211540.7019082293546001No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACT1138490.6595865592864607No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTA1134480.6572633574113993No Hit
CTTCGGTACGCCCCTCCCCGCCTTCATTGACCTCA960470.5564503004838575No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCT958180.5551235842010918No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC899250.5209823656231937No Hit
AACTTTTGACGGTGGATCTCTTGGCTCACGTATCG833950.48315067424127034No Hit
TCAGGTCTAGGCTGGTTAGTTT794310.46018515745138616No Hit
GATTCTATAGACACCCCTCCTCCAAATACTTGACC791660.45864987441674454No Hit
TCCCGTAGATCCGAACTTGT790660.4580705223282005No Hit
GAGGTCCGGACGGAGGAGGGTTATCCAC697310.40398800486261793No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACGATTCT555360.3217489758937969No Hit
GGTGACCGCCTGGGAATACCCCGTGTTGTAGGCTT491030.28447925603776125No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT450400.26094018068021846No Hit
TTACCGTGACCGATCCCGT436720.25301464410893654No Hit
CTGGCAACCTACCGCTAGTGGATGTGT428260.24811332543985426No Hit
GGGGACTAGTTGGGAAGTGCTTAGGGTACTGTCGG428020.2479742809386037No Hit
AGCTGTCGACTCCTGCACCAAGAAGAGTGT426600.24715160097287123No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCC403880.2339887215211515No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGC402040.23292271367823053No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACGATT389740.22579668298913927No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCTGTGTTT388260.22493924189809417No Hit
TGAAAATCTTTTCTCTGAAGTGGAA384890.22298682535970088No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC382530.22161955443073703No Hit
GAGGTCCGGACGGAGGAGGGTTAT355930.2062087888754666No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCC333740.19335296603067517No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTACT327650.18982471181144223No Hit
TGACTGTTAGTGAGGAATGTGAAATTGTCCTCTGA318020.18424555119876349No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCT301090.17443712033971354No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACGATTC300760.174245934150494No Hit
TCTCAGCTCACCAATCTCTGCT297490.17235145282095513No Hit
GCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAG287850.16676649868739093No Hit
GAGGTCCGGATTTGGGGAGGGTTATCTACT278390.16128582792976467No Hit
CGATTCTATAGACACCCCTCCTCCAAATACTTGAC268400.1554981005652101No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTACTTA268070.1553069143759906No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACT260730.1510544700460776No Hit
CACCCCTCTTCCACTAACTTGACCTCAT257920.14942649067726896No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGC253290.14674409050731024No Hit
GAGGTCCGGACGGTTGAGGGTTATC245150.14202816450656205No Hit
TGGGTGACCGCCTGGGAATACCCCGTGTTGTAGGC235660.13653011318627947No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAC227940.1320575150627198No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA219810.12734738258285705No Hit
TGACCACTTTGGATTCTTGCGCCATTCTT216120.1252095733761297No Hit
GACTGTTAGTGAGGAATGTGAAATTGTCCTCTGAT212630.12318763458711113No Hit
CACCCCTCTTCCACTAACTTGACCTCATT212120.12289216502195369No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGCG192820.11171066971305443No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACG192350.11143837423143875No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGT189600.10984515598794276No Hit
CCACGATTCTATAGACACCCCTCCTCCAAATACTT187000.10833884055772836No Hit
GTGACCTCTGGCAGCATGAATGTGCACAACTCTGC184540.10691363441991011No Hit
ACGATTCTATAGACACCCCTCCTCCAAATACTTGA182420.10568540799219682No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)