FastQCFastQC Report
Wed 21 Jun 2023
sRNA-POR-79-S1-TP2.flexbar_trim.20230621_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POR-79-S1-TP2.flexbar_trim.20230621_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15794990
Sequences flagged as poor quality0
Sequence length18-35
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGAGAATTCTACCACTGAACCACCAGTGC5012423.1734239781095144No Hit
TTCCACTTCAGAGCAAAGATTTTCA4387052.777494635957351No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGAT3943582.4967283929904354No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT2698191.7082568586621456Illumina RNA PCR Primer (100% over 35bp)
AGCGAGAATTCTACCACTGAACCACCAGTGC2506091.586636015597351No Hit
AGACAACTACTCACGCTTAAGCACAC2070321.3107447361473479No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGC2009741.2723908024000015No Hit
AGCAGACAACTACTCACGCTTAAGCACAC1809821.1458190223608877No Hit
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGC1235110.7819631414771393No Hit
GCTGAGTTCATCAACAGAATATTTAAACAA1149970.7280599734472766No Hit
AATGAGGTCAATGAAGGCGGGGAGGGGCGTACCGA768330.48643905440902463No Hit
AGTAGATAACCCTCCCCAAGTCCGGACCTC663710.42020286179351807No Hit
AAGGATGATAACCCTCGACCGTCCGGACCTC621260.3933272512359932No Hit
AACAGAGTCGAGTTCGACTATCTTGAAC605260.3831974569151358No Hit
GGCGAGAATTCTACCACTGAACCACAGTGC602040.3811588358080632No Hit
ACACTCTTCTTGGTGCAGGAGTCGACAGCT569370.3604750620291624No Hit
GTCTCATGCTCTACCGACTGAGCTAGCCGGGC546310.3458754959642266No Hit
TCCGACACTCAGACAGACATGCTCCTGGGAGAACC542770.3436342789707369No Hit
AAAGAGGTCTAGTATTTGGAGAAGGGGTGTCTATA502010.3178286279383526No Hit
GCTCTTCTAGGTGCAGGAGTCGACAGCT499950.3165244169195422No Hit
GCAAATCTTAGAACAAAGGCTTAATCTCAGAAGAT489200.3097184613602161No Hit
CGTGGATAACCCTCCTCCGTCCGGACCTC466670.2954544447321587No Hit
AAGCCTACAACACGGGGTATTCCCAGGCGGTCACC458410.2902249384140161No Hit
GTGGATAACCCTCCTCCGTCCGGACCTC451400.28578682227719043No Hit
AAACTAACCAGCCTAGACCTGA442210.27996852166414793No Hit
AGCCTACTTCGTCAGGGGCTCAAGTCG437340.27688526551773696No Hit
AGCCGACAGTACCCTAAGCACTTCCCAACTAGTCC435110.27547342543426745No Hit
ACAAGTTCGGATCTACGGGA394350.24966777440188315No Hit
ACTGAGTTCATCAACAGAATATTTAAACAA385220.24388746051754387No Hit
ACAGAGTCGAGTTCGACTATCTTGAAC382000.2418488394104713No Hit
AGTGAGGTCTAAGAGCGGGGAGGGGGGAGATCAAC381070.24126004511557148No Hit
CGCTCTTCTAGGTGCAGGAGTCGACAGCT377850.23922142400849888No Hit
GGGCGAGAATTCTACCACTGAACCACCAGTGC317620.20108907951192118No Hit
TTCCACTTCAGAGAAAAGATTTTCA317190.20081684128954816No Hit
AAGAGGTCTAGTATTTGGAGAAGGGGTGTCTATAG316040.20008876232273648No Hit
GCGAGAATTCTACCACTGAACCACCAGTGC314600.19917708083385935No Hit
AAAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATA311700.19734105561320395No Hit
AGCGAGAATTCTACCACTGAACCACAGTGC309390.19587856655813013No Hit
AGTCTGATGCTCTACCGACTGAGCTAACCGGGC308280.19517581207712068No Hit
ATCATCAGAGGACAATTTCACATTCCTCACTAACA305000.19309920424134489No Hit
GGCAGACAACTACTCACGCTTAAGCACAC302330.1914087948140518No Hit
GGACAACTACTCACGCTTAAGCACAC295320.18697067867722614No Hit
AGAATTCTACCACTGAACCACCAGTGC285050.18046861694752575No Hit
AAGAGGTCTAGTATTTGGAGAAGGGGTG271440.1718519606533464No Hit
AGAATCGTGGATAACCCTCCTCCGTCCGGACCTC264180.16725556648025736No Hit
AGGCGAGAATTCTACCACTGAACCACAGTGC262010.16588171312549105No Hit
ATGAGGTCAAGTTAGTGGAAGAGGGGTG260720.16506499845837194No Hit
AGGATGATAACCCTCGACCGTCCGGACCTC239860.15185827911255403No Hit
AGTAGATAACCCTCCCCAAATCCGGACCTC236820.14993361819159112No Hit
AATCGTGGATAACCCTCCTCCGTCCGGACCTC230750.14609062747111584No Hit
ATCAGAGGACAATTTCACATTCCTCACTAACAGTC223690.1416208557270375No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGC215980.13673956108867433No Hit
AATGAGGTCAAGTTAGTGGAAGAGGGGTG211540.13392854316463637No Hit
ACTCTTCTTGGTGCAGGAGTCGACAGCT190230.12043692335354439No Hit
AAACACAGCAGACAACTACTCACGCTTAAGCACAC188790.11952524186466722No Hit
AAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATAG188270.11919602354923936No Hit
AGTAGTAGATAACCCTCCCCAAGTCCGGACCTC176370.11166198902310162No Hit
AAGCCTACAACACGGGGTATTCCCA174990.11078829426292769No Hit
TCTCATGCTCTACCGACTGAGCTAGCCGGGC171770.10874967315585513No Hit
AACCGGTGTTCTCCCTGTGGTATGGTCGTAGGC170840.10816087886095528No Hit
ACACCAAGAAGATGGAATCACACAA164820.10434954374773267No Hit
ACTCTTCTAGGTGCAGGAGTCGACAGCT164750.10430522589757892No Hit
AATTCAGAAAAACTGAACAGTCATCAGTGCT161420.10219696245455047No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)