FastQCFastQC Report
Wed 21 Jun 2023
sRNA-POR-79-S1-TP2.flexbar_trim.20230621_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POR-79-S1-TP2.flexbar_trim.20230621_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15794990
Sequences flagged as poor quality0
Sequence length18-35
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC5109243.2347218959936033No Hit
TGAAAATCTTTGCTCTGAAGTGGAA4438372.8099859512415013No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT2505211.5860788769097036No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCGTACG2435941.5422231986218413TruSeq Adapter, Index 22 (100% over 35bp)
GTGTGCTTAAGCGTGAGTAGTTGTCT2074581.3134417938852763No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCT2014011.2750941912593803No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCT1813991.1484591000057613No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTA1773491.122818058131091No Hit
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCT1504690.9526375135406859No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACT1227850.7773667473040502No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGC1161830.7355686834876123No Hit
CTTCGGTACGCCCCTCCCCGCCTTCATTGACCTCA1058920.6704151126401473No Hit
AACTTTTGACGGTGGATCTCTTGGCTCACGTATCG738400.46749000790757067No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACT663690.420190199550617No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTT621350.39338423132904804No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC612640.38786982454563124No Hit
GTTCAAGATAGTCGAACTCGACTCTGTT608190.38505247550014277No Hit
GCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAG593420.3757014091177012No Hit
AGCTGTCGACTCCTGCACCAAGAAGAGTGT573010.36277959023715745No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAC550870.348762487345671No Hit
GATTCTATAGACACCCCTTCTCCAAATACTAGACC513240.3249384773273044No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGC512130.3242357228462949No Hit
GGGGACTAGTTGGGAAGTGCTTAGGGTACTGTCGG473770.29994954096203924No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACG469540.29727147658846254No Hit
GAGGTCCGGACGGAGGAGGGTTATCCAC459480.29090236840922346No Hit
TCAGGTCTAGGCTGGTTAGTTT441800.27970894568467597No Hit
CGACTTGAGCCCCTGACGAAGTAGGCT439000.27793623167852594No Hit
TGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAA421730.2670023849334504No Hit
TCCCGTAGATCCGAACTTGT394550.24979439683089386No Hit
GTTCAAGATAGTCGAACTCGACTCTGT385670.24417236098281794No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGCG382500.2421653954829981No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGT380720.2410384558648027No Hit
GGTGACCGCCTGGGAATACCCCGTGTTGTAGGCTT349400.22120938348172428No Hit
TGACTGTTAGTGAGGAATGTGAAATTGTCCTCTGA339580.214992222217298No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCC324440.20540690434118664No Hit
GATTCTATAGACACCCCTCCTCCAAATACTTGACC323180.20460918303841913No Hit
TGAAAATCTTTTCTCTGAAGTGGAA321310.20342526332716893No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC319010.20196910539354568No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT308960.19560632833575708No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCC308380.19523912329162602No Hit
GCCCGGTTAGCTCAGTCGGTAGAGCATCAGACT307130.19444773311030902No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCC300440.1902122128599005No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT285470.18073452404844828No Hit
CACCCCTTCTCCAAATACTAGACCTCTT276590.1751124882003724No Hit
CACCCCTCTTCCACTAACTTGACCTCAT265520.16810393675462917No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACGATTCT265100.16783802965370664No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT262170.16598301106869964No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCT240210.1520798683633228No Hit
GAGGTCCGGATTTGGGGAGGGTTATCTACT236980.1500349161347997No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACGATT230930.1462045876572255No Hit
GACTGTTAGTGAGGAATGTGAAATTGTCCTCTGAT216820.13727137529051933No Hit
CACCCCTCTTCCACTAACTTGACCTCATT216370.1369864748252452No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA213200.13497950932542535No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCTGTGTTT195840.12398868248729503No Hit
AGCTGTCGACTCCTGCACCAAGAAGAGT191880.1214815583928828No Hit
GTGACCTCTGGCAGCATGAATGTGCACAACTCTGC186120.11783483243737412No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTACT176700.11187091603096931No Hit
TGGGAATACCCCGTGTTGTAGGCTT174860.11070598968407071No Hit
GCCTACGACCATACCACAGGGAGAACACCGGTT174100.11022482445382999No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA170450.10791396512438438No Hit
TTGTGTGATTCCATCTTCTTGGTGT165330.10467243094171No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGT164920.10441285496223802No Hit
AGCACTGATGACTGTTCAGTTTTTCTGAATT162330.10277309450654924No Hit
GAAACTCATGAATCACCCCTCTTCCACTAACTTGA160500.10161449928110117No Hit
CACCCCTTCTCCAAATACTAGACCTCTTT158140.10012035461877469No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)