FastQCFastQC Report
Wed 21 Jun 2023
sRNA-POR-73-S1-TP2.flexbar_trim.20230621_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POR-73-S1-TP2.flexbar_trim.20230621_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14761972
Sequences flagged as poor quality0
Sequence length18-35
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCA11863418.036466943576373No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT4339342.939539514097439Illumina RNA PCR Primer (100% over 35bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGAT3489532.363864394269275No Hit
CCGCTTCAAGTCTCAGCCCGAG2920871.9786448585595473No Hit
ACCCGCTTCAAGTCTCAGCCCGAG2877301.9491298317054115No Hit
ACCCGCTTCAAGTCTCAGCCCGAGA2228541.5096492528234033No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGC1745671.1825452588583694No Hit
CAGCCCAGGCCGGCTCCAC1003910.6800649669298926No Hit
AGTAGATAACCCTCCCCAAGTCCGGACCTC772320.5231821331188001No Hit
CGCTTCAAGTCTCAGCCCGAG767420.5198627933991475No Hit
ACCCGCTTCAAGTCTCAGCCCG752440.5097150976847808No Hit
TGTCTTTCACCTACAGCATTAATA646750.4381189721806815No Hit
AGCCCAGGCCGGCTCCAC594290.4025817146923189No Hit
CCGCTTCAAGTCTCAGCCCGAGA564190.3821914849858813No Hit
ACAAGTTCGGATCTACGGGA549390.3721657241999917No Hit
GTGGATAACCCTCCTCCGTCCGGACCTC518660.35134872224388447No Hit
CGGGAACAAGCCGAAACATTG469200.3178437135634724No Hit
CACCCGCTTCAAGTCTCAGCCCGAG431720.29245415178947637No Hit
AAAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATA400420.27125102255985856No Hit
AATTCAGAAAAACTGAACAGTCATCAGTGCT382640.2592065612914047No Hit
ATGAGGTCAAGTTAGTGGAAGAGGGGTG378780.2565917344918416No Hit
AAACTAACCAGCCTAGACCTGA349230.2365740837335283No Hit
CGCTCTTCTAGGTGCAGGAGTCGACAGCT347260.23523957368297407No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGC342840.23224539377259354No Hit
TCCACTTCAGAGCAAAGATTTTCA340060.23036217654389263No Hit
GCAAATCTTAGAACAAAGGCTTAATCTCAGAAGAT328260.22236866456595367No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGC327870.22210447222092006No Hit
TTTTTTTGGTCTTTCACCTACAGCATTAATA307870.2085561468345828No Hit
GTCTCATGCTCTACCGACTGAGCTAGCCGGGC307790.20850195353303747No Hit
GCCCGCTTCAAGTCTCAGCCCGAG299440.20284552768424163No Hit
TAGTAGATAACCCTCCCCAAGTCCGGACCTC285330.1932871841241807No Hit
AAGGATGATAACCCTCGACCGTCCGGACCTC276820.18752237167229419No Hit
AATGAGGTCAATGAAGGCGGGGAGGGGCGTACCGA271900.18418948362725523No Hit
ATCATCAGAGGACAATTTCACATTCCTCACTAACA269150.18232658888663386No Hit
AGTGAGGTCTAAGAGCGGGGAGGGGGGAGATCAAC263510.17850596112768674No Hit
AATGAGGTCAAGTTAGTGGAAGAGGGGTG262400.17775402906874502No Hit
ACACTCTTCTTGGTGCAGGAGTCGACAGCT261630.17723241854137103No Hit
CCGCTTCAAGTCTCAGCCCG253790.17192147498992683No Hit
TCGCTTCAAGTCTCAGCCCGAG252690.17117631709367828No Hit
AGTAGTAGATAACCCTCCCCAAGTCCGGACCTC238480.16155023190668566No Hit
CCACTTCAGAGCAAAGATTTTCA237630.1609744280777663No Hit
GCCCGCTTCAAGTCTCAGCCCGAGA232320.15737734768769376No Hit
AAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATAG230690.15627315916870727No Hit
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGC221150.14981060795942439No Hit
AAGAGGTCAAGTATTTGGAGGAGGGGTG214210.14510933905036535No Hit
ACACATCCACTAGCGGTAGGTTGCCAG210760.14277225292122217No Hit
TTTTTTTTTCCTTCACGAGTAAACATTCTA205950.13951388066580805No Hit
GTAGTAGATAACCCTCCCCAAGTCCGGACCTC204240.13835549884527623No Hit
AATGAGGTCAAGTTAGTG201400.13643163664041633No Hit
GCTATTCAACCTCCTGACGATTTTAGGCA184940.12528136484746075No Hit
GCTGAGTTCATCAACAGAATATTTAAACAA179900.12186718685010378No Hit
ACGGGATCGGTCACGGTAA178770.1211017064657757No Hit
GCTCTTCTAGGTGCAGGAGTCGACAGCT175300.1187510720112462No Hit
CGCTTCAAGTCTCAGCCCGAGA171960.11648850167172786No Hit
TTTTTTGAGCCAAGACGCTATCACTA171510.11618366435053529No Hit
ACAGAGTCGAGTTCGACTATCTTGAACGGGCTGT165010.11178045859997568No Hit
ACACGTCAGATGCTGAACAGATGCTGATA162810.11029014280747856No Hit
ATCAGAGGACAATTTCACATTCCTCACTAACAGTC161740.10956530739930953No Hit
AGAATCGTGGATAACCCTCCTCCGTCCGGACCTC161620.1094840174469915No Hit
TCCGACACTCAGACAGACATGCTCCTGGGAGAACC160580.10877950452690195No Hit
GGCGAGAATTCTACCACTGAACCACAGTGC158000.10703177055206446No Hit
GATCTCATTGTGGAAGAAACAAACCGTTA150220.10176147197677925No Hit
TTCCACCTCAGAGCAAAGATTTTCA148310.10046760690238404No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)