FastQCFastQC Report
Wed 21 Jun 2023
sRNA-POR-73-S1-TP2.flexbar_trim.20230621_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POR-73-S1-TP2.flexbar_trim.20230621_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14761972
Sequences flagged as poor quality0
Sequence length18-35
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA11947408.093363136036297No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCC3839802.601142990922893TruSeq Adapter, Index 20 (100% over 35bp)
CTCGGGCTGAGACTTGAAGCGG2924191.9808938805736795No Hit
CTCGGGCTGAGACTTGAAGCGGGT2875091.9476327417502215No Hit
TCTCGGGCTGAGACTTGAAGCGGGT2227111.5086805475582803No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTA1809571.2258321584677168No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC1770601.1994332464524389No Hit
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCT1083550.7340143986182877No Hit
GTGGAGCCGGCCTGGGCTG1005850.6813791544923673No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACT772090.5230263273768573No Hit
CTCGGGCTGAGACTTGAAGCG769520.5212853675647129No Hit
CGGGCTGAGACTTGAAGCGGGT752530.5097760651490194No Hit
TATTAATGCTGTAGGTGAAAGACA649810.44019186596479115No Hit
GTGGAGCCGGCCTGGGCT595740.4035639682828283No Hit
TCTCGGGCTGAGACTTGAAGCGG564770.3825843864220851No Hit
TCCCGTAGATCCGAACTTGT549510.37224701415230976No Hit
GAGGTCCGGACGGAGGAGGGTTATCCAC525260.3558196696213758No Hit
GATTCTATAGACACCCCTCCTCCAAATACTTGACC476180.32257207912330416No Hit
CAATGTTTCGGCTTGTTCCCG471980.3197269307921733No Hit
CTCGGGCTGAGACTTGAAGCGGGTG432080.29269802164643044No Hit
CTTCGGTACGCCCCTCCCCGCCTTCATTGACCTCA412670.2795493718589901No Hit
GCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAG397480.26925941872806697No Hit
CACCCCTCTTCCACTAACTTGACCTCAT385630.2612320359366621No Hit
AGCACTGATGACTGTTCAGTTTTTCTGAATT385230.2609610694289354No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGCG350520.23744795072094702No Hit
TCAGGTCTAGGCTGGTTAGTTT348660.2361879564600177No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT341660.23144604257479962No Hit
TGAAAATCTTTGCTCTGAAGTGGA341330.23122249520592508No Hit
TGACTGTTAGTGAGGAATGTGAAATTGTCCTCTGA340470.23063991721431254No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCT327830.22207737557014742No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA311250.2108458138248738No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAC307420.20825130951339024No Hit
CTCGGGCTGAGACTTGAAGCGGGC302200.2047151965875562No Hit
AACTTTTGACGGTGGATCTCTTGGCTCACGTATCG300310.20343487983854733No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTA285260.19323976498532852No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTT276400.18723785683918112No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTACTTA276220.18711592191070409No Hit
CACCCCTCTTCCACTAACTTGACCTCATT267190.1809988529987728No Hit
AGCTGTCGACTCCTGCACCAAGAAGAGTGT263410.17843821950075503No Hit
CGGGCTGAGACTTGAAGCGG254210.17220598982303992No Hit
CTCGGGCTGAGACTTGAAGCGA253140.17148115441487086No Hit
TGAAAATCTTTGCTCTGAAGTGG238370.16147571611706077No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTACT238160.16133345870050422No Hit
TCTCGGGCTGAGACTTGAAGCGGGC234610.15892863094442938No Hit
CACCCCTCCTCCAAATACTTGACCTCTT220620.14945157733668646No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACT217950.14764287589761044No Hit
TGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAA216380.14657933235478296No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAA211790.14346999167861854No Hit
CTGGCAACCTACCGCTAGTGGATGTGT210860.14283999454815385No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTAC206140.13964258975697827No Hit
GACTGTTAGTGAGGAATGTGAAATTGTCCTCTGAT203400.13778646917905008No Hit
CACTAACTTGACCTCATT202990.13750872850863013No Hit
TGCCTAAAATCGTCAGGAGGTTGAATAGC186940.1266361973860945No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGC180940.12257169977019329No Hit
TTACCGTGACCGATCCCGT179710.12173847775893355No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGC179480.12158267201699069No Hit
TAGTGATAGCGTCTTGGCTCAAAAAA173360.11743688444877148No Hit
TCTCGGGCTGAGACTTGAAGCG172170.11663075908828441No Hit
ACAGCCCGTTCAAGATAGTCGAACTCGACTCTGT170440.11545882894236624No Hit
TATCAGCATCTGTTCAGCATCTGACGTGT162330.10996498299820646No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACGATTCT162050.10977530644279775No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC160180.10850853801917522No Hit
TAACGGTTTGTTTCTTCCACAATGAGATC152140.10306211121386764No Hit
TGAAAATCTTTGCTCTGAGGTGGAA149640.10136857054057546No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)