FastQCFastQC Report
Wed 21 Jun 2023
sRNA-POR-73-S1-TP2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POR-73-S1-TP2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22960890
Sequences flagged as poor quality0
Sequence length150
%GC71

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATG17008677.407670173063849TruSeq Adapter, Index 20 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCGCGTATG12653045.510692312014038TruSeq Adapter, Index 20 (97% over 42bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC10782644.696089742165918Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGACTTGAAGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT4580081.9947310404779606TruSeq Adapter, Index 1 (97% over 35bp)
GAGACTTGAAGCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3227551.4056728637261013Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCAT3190301.3894496249927593TruSeq Adapter, Index 20 (97% over 41bp)
CTCGGGCTGAGACTTGAAGCGGGTAGATCGGAAGAGCACACGTCTGAACT2667711.1618495624516298Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGGGCTGAGACTTGAAGCGGAGATCGGAAGAGCACACGTCTGAACTCC2588831.1274954934238177Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTCGGGCTGAGACTTGAAGCGGGTAGATCGGAAGAGCACACGTCTGAAC2070410.9017115625744473Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG1664900.72510255482257No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1636500.7127336962983578No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTTTG1606810.6998030128623063TruSeq Adapter, Index 20 (97% over 46bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGCCGTC1344880.5857264243676966TruSeq Adapter, Index 20 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCGCGTTTG1144490.49845193283012984TruSeq Adapter, Index 20 (97% over 42bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCGCGTATGCCGTC1087570.47366195299920866TruSeq Adapter, Index 20 (97% over 38bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG989590.43098939109067635No Hit
GAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTG926650.4035775616711722TruSeq Adapter, Index 15 (97% over 36bp)
GTGGAGCCGGCCTGGGCTGAGATCGGAAGAGCACACGTCTGAACTCCAGT886430.38606081907103773Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAGGTCCGGACTTGGGGAGGGTTATCTACTAGATCGGAAGAGCACACGTC725910.3161506370179902No Hit
CGGGCTGAGACTTGAAGCGGGTAGATCGGAAGAGCACACGTCTGAACTCC693020.30182627938202744Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA677740.2951714850774513Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTAATGCTGTAGGTGAAAGACAAGATCGGAAGAGCACACGTCTGAACT580090.25264264582078483Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGGAGCCGGCCTGGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTC544410.2371031784917745Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCGCGGATG521560.2271514736580333TruSeq Adapter, Index 20 (97% over 42bp)
TCTCGGGCTGAGACTTGAAGCGGAGATCGGAAGAGCACACGTCTGAACTC504280.2196256329785126Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG501970.21861957441545168Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAGGTCCGGACGGAGGAGGGTTATCCACAGATCGGAAGAGCACACGTCTG479570.20886385501607296Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGGATG477690.20804507142362516TruSeq Adapter, Index 20 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATT437830.19068511717098074TruSeq Adapter, Index 20 (97% over 48bp)
GAGACTTGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGG437730.19064156485223352TruSeq Adapter, Index 20 (100% over 37bp)
GGAGCCGGCCTGGGCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA435750.18977922894103844Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GAGACTTGAAGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT419430.18267149052149112TruSeq Adapter, Index 1 (97% over 35bp)
CAATGTTTCGGCTTGTTCCCGAGATCGGAAGAGCACACGTCTGAACTCCA415610.18100779194534705Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCGGGCTGAGACTTGAAGCGGGTGAGATCGGAAGAGCACACGTCTGAAC387940.16895686534798957Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCACTGATGACTGTTCAGTTTTTCTGAATTAGATCGGAAGAGCACACGT364610.15879610938426167No Hit
GCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGAA361880.15760713108246238No Hit
CACCCCTCTTCCACTAACTTGACCTCATAGATCGGAAGAGCACACGTCTG360480.15699739862000123Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGACTTGAAGCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC340080.14811272559556707Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT325480.14175408705847203No Hit
GACTTGAAGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGG321080.1398377850335941TruSeq Adapter, Index 20 (100% over 37bp)
TCAGGTCTAGGCTGGTTAGTTTAGATCGGAAGAGCACACGTCTGAACTCC320360.13952420833861406Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCTGTCGACTCCTGCACCTAGAAGAGCGAGATCGGAAGAGCACACGTCT314930.13715931743063967Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGAGCCGGCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTG314370.13691542444565521TruSeq Adapter, Index 15 (97% over 36bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG310350.1351646212320167No Hit
GACTTGAAGCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT307570.1339538667708438TruSeq Adapter, Index 1 (97% over 35bp)
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT306900.1336620662352374Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATTAATGCTGTAGGTGAAAGACCAAAAAAAAGATCGGAAGAGCACACGT286240.12466415718206045No Hit
ACTAACTTGACCTCATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA283520.12347953411213589Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCGGGCTGAGACTTGAAGCGGGCAGATCGGAAGAGCACACGTCTGAACT283020.12326177251839976Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG282700.12312240509840865No Hit
TTGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCAT267910.11668101715569387TruSeq Adapter, Index 20 (97% over 41bp)
GAGGTCCGGACTTGGGGAGGGTTATCTACTAAGATCGGAAGAGCACACGT261650.11395464200211751No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTTAGATCGGAAGAGCACACGT260910.11363235484338804No Hit
GAGACTTGAAGCGGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA255630.1113327924135345Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CACCCCTCTTCCACTAACTTGACCTCATTAGATCGGAAGAGCACACGTCT251430.109503595026151Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGC250820.10923792588179292TruSeq Adapter, Index 20 (97% over 43bp)
AACTTTTGACGGTGGATCTCTTGGCTCACGTATCGATGAAGAACGCAGCC248660.10829719579685282No Hit
AGCTGTCGACTCCTGCACCAAGAAGAGTGTAGATCGGAAGAGCACACGTC247690.10787473830500473No Hit
CTCGGGCTGAGACTTGAAGCGAAGATCGGAAGAGCACACGTCTGAACTCC233740.10179918983976666Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCGCGTGTG231770.1009412091604463TruSeq Adapter, Index 20 (97% over 42bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)