FastQCFastQC Report
Wed 21 Jun 2023
sRNA-ACR-178-S1-TP2.flexbar_trim.20230621_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-178-S1-TP2.flexbar_trim.20230621_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16505612
Sequences flagged as poor quality0
Sequence length18-35
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT7234774.383218265399671Illumina RNA PCR Primer (100% over 35bp)
GGCGAGAATTCTACCACTGAACCACCAGTGC5474813.316938505521637No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGC3948002.3919137321294115No Hit
GGCGAGAATTCTACCACTGAACCACAGTGC2174551.3174609944787263No Hit
TTCCACCTCAGAGCAAAGATTTTCA2079891.2601108035254918No Hit
AGCGAGAATTCTACCACTGAACCACAGTGC1645000.9966307217205881No Hit
GCTTGTCAGTGGTAACTCAGGTCTTGGA1598360.9683736658780057No Hit
ACTTGGTAGACAGGTTCCGCACATAAAAGA1470710.8910363335815722No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGC1434530.86911651624914No Hit
ACAAGTTCGGATCTACGGGA1299470.7872898017958982No Hit
ACTTGTCAGTGGTAACTCAGGTCTTGGA876040.5307528130432243No Hit
CCACAAGTTCGGATCTACGGGA651910.3949626345269718No Hit
TGCGAGAATTCTACCACTGAACCACAGTGC612230.3709223262972618No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGC589420.357102784192431No Hit
GAGTGGATAACCCTCCCCAAGTCCGGACCTC573080.3472031209748539No Hit
CCAGGTCAACAGTCCGGAAATCATGTCAA469730.28458805405094945No Hit
GCTCGTCAGTGGTAACTCAGGTCTTGGA442890.26832691814153875No Hit
ACTGCTTGTCAGTGGTAACTCAGGTCTTGGA353950.21444221516899825No Hit
ACTCTTCATTTATCTACTCGTTAA302720.18340428697827138No Hit
GCAAGTTCGGATCTACGGGA288160.17458304484559553No Hit
ACTCGTCAGTGGTAACTCAGGTCTTGGA281930.170808571048441No Hit
AGTGGATAACCCTCCCCAAGTCCGGACCTC279720.16946963251044553No Hit
GCTTGGTAGACAGGTTCCGCACATAAAAGA276110.16728249761353897No Hit
AAGCACAAAGAACTCAAAGAAGGAAGTA270940.16415022963098855No Hit
CTGCTTGTCAGTGGTAACTCAGGTCTTGGA260930.15808562566477388No Hit
AGGGACGCTCGGACAGCCTTGCTCGCCTCGCAAGA256180.1552078165898968No Hit
AGTGAATCCATGTACGATTTTCGATGTGA250420.15171809442751957No Hit
GAGAATTCTACCACTGAACCACCAGTGC239580.14515063119137903No Hit
GGCAGTCTGCTCAGAATTTCCACCAGAA238880.14472653301192345No Hit
AGGCGAGAATTCTACCACTGAACCACAGTGC231650.1403462046726895No Hit
TCACAAGTTCGGATCTACGGGA227690.13794702068605516No Hit
AATCATACCACTCTTAGCCCGTCATTTAA227290.13770467886922338No Hit
AGCAGTCTGCTCAGAATTTCCACCAGAA223880.13563871488073267No Hit
ACACGCTTTGTCCATAACAAGAGACCATA211990.1284351043754088No Hit
AGAGTGGATAACCCTCCCCAAGTCCGGACCTC211660.1282351723765226No Hit
GCGAGAATTCTACCACTGAACCACCAGTGC204010.1236003851296153No Hit
ACTTTTTATTTACGGAATCCACAATCTTA198240.12010460442181727No Hit
ATTGTAAGCTTATATATACGAGTTAGATCA195430.11840215315857419No Hit
AGCCGGAGATCCTAACCACCTAGACTACGTTGGA194260.1176933033443413No Hit
AGTTTTGGCTTAACAAGTCTGGAGGCTCTA193760.1173903760733016No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG183540.11119854265325028No Hit
GAATGCTCTACCACTGAGCTACACCCCC179400.10869030484904164No Hit
AATCAGAATCAAGTTTTCTTCAAGACTTTGTCATT173470.10509758741451089No Hit
TCAGGTCAACAGTCCGGAAATCATGTCAA171730.10404340051129277No Hit
AAGAGTGGATAACCCTCCCCAAGTCCGGACCTC169530.10271052051871812No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)