FastQCFastQC Report
Wed 21 Jun 2023
sRNA-ACR-178-S1-TP2.flexbar_trim.20230621_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-178-S1-TP2.flexbar_trim.20230621_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16505612
Sequences flagged as poor quality0
Sequence length18-35
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCACACGTCTGAACTCCAGTCACCAGGCG7006084.244665390171537TruSeq Adapter, Index 7 (96% over 32bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC5585533.3840187204206664No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT3948042.3919379663110947No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC2213181.340865155439253No Hit
TGAAAATCTTTGCTCTGAGGTGGAA2092151.267538580211385No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT1642400.9950554999111818No Hit
TCCAAGACCTGAGTTACCACTGACAAGC1627170.9858283352353127No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGT1472160.8919148226675873No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA1430490.8666688638991393No Hit
TCCCGTAGATCCGAACTTGT1300670.7880168272463935No Hit
TCCAAGACCTGAGTTACCACTGACAAGT874410.5297652701396349No Hit
TCCCGTAGATCCGAACTTGTGG653100.39568360143204623No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA609580.36931681176075143No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCT591310.3582478492769611No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC581050.3520317816752266No Hit
TTGACATGATTTCCGGACTGTTGACCTGG473000.28656919840354905No Hit
TCCAAGACCTGAGTTACCACTGACGAGC452700.2742703511993375No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT354390.21470879116751318No Hit
TTAACGAGTAGATAAATGAAGAGT303120.18364662879510316No Hit
TCCCGTAGATCCGAACTTGC292630.1772912146486904No Hit
TCCAAGACCTGAGTTACCACTGACGAGT282160.17094791759311925No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT280610.17000884305289618No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGC279370.16925758342071776No Hit
TACTTCCTTCTTTGAGTTCTTTGTGCTT271400.16442892272034507No Hit
GAGACTCTGCACGGTGGATCTCTCGGCTCGCGCAT266370.16138147437368575No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG262460.15901258311415536No Hit
TCACATCGAAAATCGTACATGGATTCACT252810.1531660867830893No Hit
TTCTGGTGGAAATTCTGAGCAGACTGCC244390.14806479153878085No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC243010.14722871227071133No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT231900.14049766830820937No Hit
TCCCGTAGATCCGAACTTGTGA227810.13801972323110465No Hit
TTAAATGACGGGCTAAGAGTGGTATGATT227440.13779555705053528No Hit
TTCTGGTGGAAATTCTGAGCAGACTGCT224530.1360325203330843No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCT212440.12870773891934453No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC212130.1285199240112999No Hit
TATGGTCTCTTGTTATGGACAAAGCGTGT211060.12787165965127495No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAA205400.12444252294310566No Hit
TAAGATTGTGGATTCCGTAAATAAAAAGT198490.1202560680573371No Hit
TGATCTAACTCGTATATATAAGCTTACAAT196790.11922611533580216No Hit
TCCAACGTAGTCTAGGTGGTTAGGATCTCCGGCT194810.11802652334248498No Hit
TAGAGCCTCCAGACTTGTTAAGCCAAAACT194550.11786900116154432No Hit
TTTTATGATGAATCTTTTAGACTAGAGTCTCTGAA192580.11667546771364794No Hit
GGGGGTGTAGCTCAGTGGTAGAGCATTC181390.10989595538777963No Hit
TTGACATGATTTCCGGACTGTTGACCTGA172920.10476436741636724No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTT169440.10265599360993097No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)