FastQCFastQC Report
Wed 21 Jun 2023
sRNA-ACR-173-S1-TP2.flexbar_trim.20230621_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-173-S1-TP2.flexbar_trim.20230621_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18566189
Sequences flagged as poor quality0
Sequence length18-35
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT4450242.3969593329034837Illumina RNA PCR Primer (100% over 35bp)
GGCGAGAATTCTACCACTGAACCACCAGTGC2842871.5312081547807146No Hit
GCTTGTCAGTGGTAACTCAGGTCTTGGA2300101.2388649065244353No Hit
AGAATTCTACCACTGAACCACCAGTGC2263431.2191139495563683No Hit
TTCCGCCTCAGAAGAAAGATTTTCA2261171.2178966830511098No Hit
AGAATCATACCACTAGACCAAACAGCC1864151.00405635211405No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGC1371060.738471422433543No Hit
ACTGCTTGTCAGTGGTAACTCAGGTCTTGGA1247940.6721573285718463No Hit
GGCGAGAATTCTACCACTGAACCACAGTGC1241510.6686940437803364No Hit
AGAATTCTACCACTGAACCACAGTGC1042160.5613214429735688No Hit
ACAAGTTCGGATCTACGGGA982090.5289669301545945No Hit
ACTTGGTAGACAGGTTCCGCACATAAAAGA960850.5175267794591556No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGC907280.48867325437654435No Hit
GAGAATTCTACCACTGAACCACCAGTGC906030.4879999875041669No Hit
TTCCGCCCCAGAAGAAAGATTTTCA828360.44616587712211697No Hit
ACTTGTCAGTGGTAACTCAGGTCTTGGA763060.4109944157091151No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGC676800.3645336153800869No Hit
CCACAAGTTCGGATCTACGGGA658320.35458003793885756No Hit
GAGTGGATAACCCTCCCCAAGTCCGGACCTC623200.3356639318925386No Hit
AGCGAGAATTCTACCACTGAACCACAGTGC621100.3345328435469444No Hit
GCTCGTCAGTGGTAACTCAGGTCTTGGA614960.33122575666982595No Hit
TTCCGTCTCAGAAGAAAGATTTTCA540700.291228318315622No Hit
AGTGAATCCATGTACGATTTTCGATGTGA537490.2894993689873565No Hit
AGGGACGCTCGGACAGCCTTGCTCGCCTCGCAAGA530250.2855998072625459No Hit
GGAGTCTCCAGATCAGGTCATTGTTACTA522140.28123165179456056No Hit
CTGCTTGTCAGTGGTAACTCAGGTCTTGGA504200.2715689256421983No Hit
CCAGGTCAACAGTCCGGAAATCATGTCAA440070.23702764202174179No Hit
GAGAATTCTACCACTGAACCACAGTGC413650.22279747340717043No Hit
AGGCGAGAATTCTACCACTGAACCACAGTGC395250.21288698504577327No Hit
GGAATTCTACCACTGAACCACCAGTGC394120.212278351793144No Hit
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCC384050.20685451386927065No Hit
AAGAGGTCAATGTGCGGGGAGGGGTG369160.19883455888550955No Hit
AGTGGATAACCCTCCCCAAGTCCGGACCTC352300.18975353531088152No Hit
GGAATCATACCACTAGACCAAACAGCC349460.1882238729768398No Hit
GCCGGATCCTAGCCACTAGACCATATGGGA337590.1818305307567428No Hit
ACTTGGTCGAGAGGTTCCACTCATAAAAGA323880.1744461397005061No Hit
TGCGAGAATTCTACCACTGAACCACAGTGC309980.16695941207966805No Hit
TTTTTTTGGCCTTTAACCTACAGCATTAATA290860.15666112199978144No Hit
ACTGCTCGTCAGTGGTAACTCAGGTCTTGGA290120.15626254801133393No Hit
AGCCGAGATATCCGTCGCTGAAAGTTGTG282450.15213138248242544No Hit
GAGAATCATACCACTAGACCAAACAGCC275240.1482479791625519No Hit
ACTCGTCAGTGGTAACTCAGGTCTTGGA249560.134416384536428No Hit
ATTTCAGATTGTGCCGAAAGTATGTTCGTA249280.13426557275701545No Hit
TGCTTGTCAGTGGTAACTCAGGTCTTGGA242920.1308399909103586No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG233900.1259816971592824No Hit
TGGCGAGAATTCTACCACTGAACCACCAGTGC232060.12499064832314268No Hit
ACTGCTCGTCAGTAGTAACTCAGGCCTTGGA224650.12099952230368871No Hit
GCTGCTTGTCAGTGGTAACTCAGGTCTTGGA223910.12060094831524122No Hit
GGGCGAGAATTCTACCACTGAACCACCAGTGC220330.11867271199275198No Hit
AGTTTTGGCTTAACAAGTCTGGAGGCTCTA220090.1185434447532555No Hit
TTTCATTTGTATCCTCACGGTTGACCCCT215610.11613045628265446No Hit
ATTGTAAGCTTATATATACGAGTTAGATCA212140.11426146744493444No Hit
CGGATCCTAGCCACTAGACCATATGGGA208930.11253251811666896No Hit
GCAAGTTCGGATCTACGGGA206800.11138527136613766No Hit
TGTCAGTGGTAACTCAGGTCTTGGA206780.11137449909617962No Hit
TCACAAGTTCGGATCTACGGGA206490.11121830118178803No Hit
AGAGTCTCCAGATCAGGTCATTGTTACTA202920.10929545099427783No Hit
ACTTGGTTGACAGGTTCCGCACATAAAAGA201510.10853600596223599No Hit
AGGCTGATCCGAGGTCCG201270.10840673872273948No Hit
ACTTGGTCGACAGGTTCTGCACATAAAAGA193700.10432943454362122No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)