FastQCFastQC Report
Wed 21 Jun 2023
sRNA-ACR-173-S1-TP2.flexbar_trim.20230621_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-173-S1-TP2.flexbar_trim.20230621_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18566189
Sequences flagged as poor quality0
Sequence length18-35
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCACACGTCTGAACTCCAGTCACCACTCA4273452.301737852609386TruSeq Adapter, Index 7 (96% over 31bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC2903371.563794271403787No Hit
TCCAAGACCTGAGTTACCACTGACAAGC2337951.2592514274200268No Hit
TGAAAATCTTTCTTCTGAGGCGGAA2271871.2236598474786615No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT2271001.2231912537354868No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT1863391.0036470058556444No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT1372900.7394624712696828No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1265010.6813514609810338No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT1248740.672588219370168No Hit
GCACTGTGGTTCAGTGGTAGAATTCT1043460.5620216405208415No Hit
TCCCGTAGATCCGAACTTGT982930.5294193654928322No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGT962340.5183293135710296No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC919400.49520124997111686No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCT911340.49086002517802657No Hit
TGAAAATCTTTCTTCTGGGGCGGAA832620.44846037062317956No Hit
TCCAAGACCTGAGTTACCACTGACAAGT760830.4097933076087936No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA675800.36399500188218487No Hit
TCCCGTAGATCCGAACTTGTGG661730.35641670996670344No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC632360.3405976315333211No Hit
TCCAAGACCTGAGTTACCACTGACGAGC627590.3380284451483285No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT621830.3349260314004129No Hit
TGAAAATCTTTCTTCTGAGACGGAA542850.29238633733611136No Hit
TCACATCGAAAATCGTACATGGATTCACT542040.29195006040281074No Hit
TAGTAACAATGACCTGATCTGGAGACTCC532780.286962499412238No Hit
GAGACTCTGCACGGTGGATCTCTCGGCTCGCGCAT510370.2748921709242538No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG507030.273093201841261No Hit
TTGACATGATTTCCGGACTGTTGACCTGG442940.23857346276072058No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC418770.22555517451642876No Hit
GCACTGGTGGTTCAGTGGTAGAATTCC401640.21632872529736719No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT397030.21384571707203887No Hit
CACCCCTCCCCGCACATTGACCTCTT375160.20206623987292172No Hit
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGC368210.19832287606250265No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT353850.19058838623262964No Hit
GGCTGTTTGGTCTAGTGGTATGATTCC353620.1904645051281122No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGC343580.1850568256091759No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGT323640.17431687246100963No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA309450.16667394692577997No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA291900.15722128003759953No Hit
TCCAAGACCTGAGTTACCACTGACGAGCAGT291610.15706508212320797No Hit
CACAACTTTCAGCGACGGATATCTCGGCT288530.15540615254966972No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTC277570.14950294861266358No Hit
TACGAACATACTTTCGGCACAATCTGAAAT250920.13514889889357476No Hit
TCCAAGACCTGAGTTACCACTGACGAGT249090.13416323619241408No Hit
TCCAAGACCTGAGTTACCACTGACAAGCA247560.13333915754062398No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAA244680.13178795066666615No Hit
AGACTCTGCACGGTGGATCTCTCGGCTCGCGCATC240600.12959040759522594No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCA234390.12624561777325438No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGC226740.12212522451430392No Hit
TCCAAGGCCTGAGTTACTACTGACGAGCAGT225580.1215004328567376No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCC224620.12098336389875165No Hit
TAGAGCCTCCAGACTTGTTAAGCCAAAACT221160.11911976119601067No Hit
CGGACCTCGGATCAGCCTGGATCACCCGCCGAACT220040.11851651407836039No Hit
AGGGGTCAACCGTGAGGATACAAATGAAA217950.11739081186774518No Hit
AGTGATTGATAGGCGGGATGGATTCTTGAAAAAAA217710.1172615446282487No Hit
TGATCTAACTCGTATATATAAGCTTACAAT213490.11498859566710218No Hit
TCCCATATGGTCTAGTGGCTAGGATCCG211920.114142972475396No Hit
TCCCGTAGATCCGAACTTGC210010.11311422069440315No Hit
TCCAAGACCTGAGTTACCACTGACA208570.11233861725742424No Hit
TCCCGTAGATCCGAACTTGTGA206950.11146606339082296No Hit
TAGTAACAATGACCTGATCTGGAGACTCT203350.10952705479837568No Hit
CGGACCTCGGATCAGCCT202730.10919311442967644No Hit
TCTTTTATGTGCGGAACCTGTCAACCAAGT201650.10861141185194226No Hit
TCTTTTATGTGCAGAACCTGTCGACCAAGT193810.10438868202839044No Hit
GCACTGTGGTTCAGTGGTAGAATTCC186140.10025751649948193No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)