FastQCFastQC Report
Wed 21 Jun 2023
sRNA-ACR-150-S1-TP2.flexbar_trim.20230621_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-150-S1-TP2.flexbar_trim.20230621_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21281386
Sequences flagged as poor quality0
Sequence length18-35
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT5711292.683702085944966Illumina RNA PCR Primer (100% over 35bp)
TTCCACCTCAGAGCAAAGATTTTCA3949191.8557015036520648No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGC2679501.2590815278666532No Hit
ACTTGGTAGACAGGTTCCGCACATAAAAGA2341311.1001680059747987No Hit
ACAAGTTCGGATCTACGGGA2030830.9542752525610879No Hit
TTCCGCCTCAGAAGAAAGATTTTCA2010200.94458133506906No Hit
GAGTGGATAACCCTCCCCAAGTCCGGACCTC1806760.8489860575810242No Hit
GCTTGTCAGTGGTAACTCAGGTCTTGGA1563120.7345010329684354No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGC1446710.6798006483224355No Hit
GGCGAGAATTCTACCACTGAACCACAGTGC1275830.5995051262168732No Hit
CCACAAGTTCGGATCTACGGGA1166030.5479107422796616No Hit
AGAATCATACCACTAGACCAAACAGCC1103500.5185282575110475No Hit
AGTGGATAACCCTCCCCAAGTCCGGACCTC1077810.5064566753312026No Hit
ACTGCTTGTCAGTGGTAACTCAGGTCTTGGA750670.352735484427565No Hit
AAGAGGTCAATGTGCGGGGAGGGGTG747820.3513962859373915No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGC738980.34724242114681814No Hit
AGATTTCACTTGACTCAAATCCAGACAGA723840.34012822285165073No Hit
AGCGAGAATTCTACCACTGAACCACAGTGC713930.3354715712595035No Hit
ACGACCAAAGTATAGAGCCGGTAGCGAA705580.3315479546303986No Hit
ACTTGTCAGTGGTAACTCAGGTCTTGGA615450.2891963897464197No Hit
TTCCGTCTCAGAAGAAAGATTTTCA602070.28290920525571034No Hit
GGAGTCTCCAGATCAGGTCATTGTTACTA578520.2718431966790133No Hit
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCC563650.26485586981975706No Hit
AGGGACGCTCGGACAGCCTTGCTCGCCTCGCAAGA552020.2593910001914349No Hit
ACTCTTCATTTATCTACTCGTTAA513150.24112621236229634No Hit
ACATGAATGTTGTCAGTAGGTCAGATCAGA495060.23262582615624752No Hit
AGAGTGGATAACCCTCCCCAAGTCCGGACCTC463400.21774897556014447No Hit
GCTCGTCAGTGGTAACTCAGGTCTTGGA439340.2064433209378374No Hit
TCATGGAAAATCGGCAGTTTT412460.1938125646515692No Hit
AGAATTCTACCACTGAACCACCAGTGC402040.18891626701381198No Hit
AATCAGAATCAAGTTTTCTTCAAGACTTTGTCATT395820.18599352504578415No Hit
TCACAAGTTCGGATCTACGGGA379750.1784423251380338No Hit
GCAAGTTCGGATCTACGGGA375640.17651105994694144No Hit
ACTCGTCGTATGTCCCTTAAATTCCATAA374410.17593309007223495No Hit
TGCGAGAATTCTACCACTGAACCACAGTGC367730.17279419676895105No Hit
AAGAGTGGATAACCCTCCCCAAGTCCGGACCTC363840.17096630830341597No Hit
ACTGCTCGTCAGTAGTAACTCAGGCCTTGGA348230.16363125973092166No Hit
GCTTGGTAGACAGGTTCCGCACATAAAAGA346660.16289352582580852No Hit
AAGAGGTCAATGTGCGGGGAGGGGT333280.15660634133509913No Hit
CTGCTTGTCAGTGGTAACTCAGGTCTTGGA328900.1545482047080956No Hit
AAAATCCAGAGACTTCAAATCGTTAGCAC326750.15353793216287698No Hit
GAGAATTCTACCACTGAACCACCAGTGC317480.14918201286325994No Hit
AGGCTGATCCGAGGTCCG308830.14511742797203153No Hit
AGTGGATAACCCTCCCCAGGTCCGGACCTC293730.13802202544514722No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGC291590.1370164518419994No Hit
ACTTGGTCGAGAGGTTCCACTCATAAAAGA271730.12768435288942176No Hit
TAAGAGTGGATAACCCTCCCCAAGTCCGGACCTC267200.12555573213135648No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG266480.12521740830226No Hit
TGGATAACCCTCCCCAAGTCCGGACCTC259880.12211610653554236No Hit
AAGTGGATAACCCTCCCCAAGTCCGGACCTC258710.12156633031326061No Hit
AGAGTCTCCAGATCAGGTCATTGTTACTA258230.12134078109386297No Hit
TTCATGGAAAATCGGCAGTTTT254190.1194424084972661No Hit
AGTGAATCCATGTACGATTTTCGATGTGA252860.11881744920185179No Hit
CAGGCTGATCCGAGGTCCG249900.11742656234889964No Hit
ACTTGGTTGACAGGTTCCGCACATAAAAGA234920.11038754712686476No Hit
GATAACCCTCCCCAAGTCCGGACCTC232060.10904364969462046No Hit
AGGGTGATAACCCTCCCCAAGTCCGGACCTC223090.10482869865712693No Hit
GAGGGACGCTCGGACAGCCTTGCTCGCCTCGCAAG220610.10366336102357243No Hit
AGGAGTGCCTGCAAATTTTCCTAAATCTTA216990.10196234399394852No Hit
ATACGACCAAAGTATAGAGCCGGTAGCGAA216710.10183077361596655No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)