FastQCFastQC Report
Wed 21 Jun 2023
sRNA-ACR-150-S1-TP2.flexbar_trim.20230621_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-150-S1-TP2.flexbar_trim.20230621_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21281386
Sequences flagged as poor quality0
Sequence length18-35
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCACACGTCTGAACTCCAGTCACCACGAT5475542.572924526626226TruSeq Adapter, Index 7 (96% over 31bp)
TGAAAATCTTTGCTCTGAGGTGGAA3971501.8661848434119845No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC2737681.286419972834476No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGT2344321.1015823875381048No Hit
TCCCGTAGATCCGAACTTGT2033740.9556426447036861No Hit
TGAAAATCTTTCTTCTGAGGCGGAA2019520.9489607490790308No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC1830870.860315206913685No Hit
TCCAAGACCTGAGTTACCACTGACAAGC1591240.7477144580714808No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT1446850.6798664335114264No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1302150.6118727417471775No Hit
TCCCGTAGATCCGAACTTGTGG1169540.5495600709465069No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT1102010.5178281151425006No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT1082330.508580597147197No Hit
GAGACTCTGCACGGTGGATCTCTCGGCTCGCGCAT802260.3769773265707412No Hit
CACCCCTCCCCGCACATTGACCTCTT758480.35640535818484753No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT751620.3531818839242895No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA737480.34653757983620054No Hit
TCTGTCTGGATTTGAGTCAAGTGAAATCT726020.3411525922230817No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT713600.3353165061711676No Hit
TTCGCTACCGGCTCTATACTTTGGTCGT706060.33177350384979626No Hit
TCCAAGACCTGAGTTACCACTGACAAGT614170.2885949251613593No Hit
TGAAAATCTTTCTTCTGAGACGGAA605140.2843517804714411No Hit
TAGTAACAATGACCTGATCTGGAGACTCC589340.2769274519996019No Hit
TTAACGAGTAGATAAATGAAGAGT514170.2416055044535163No Hit
TCTGATCTGACCTACTGACAACATTCATGT497050.2335609156283336No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCT464820.2184162253341958No Hit
TCCAAGACCTGAGTTACCACTGACGAGC449290.21111876829826778No Hit
TTTTATGATGAATCTTTTAGACTAGAGTCTCTGAA434990.20439928113704622No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAA424910.19966274752969568No Hit
AAAACTGCCGATTTTCCATGA418830.19680579075065882No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT403760.18972448504998687No Hit
TCCCGTAGATCCGAACTTGC381890.1794478987411816No Hit
TCCCGTAGATCCGAACTTGTGA380300.1787007669519269No Hit
TTATGGAATTTAAGGGACATACGACGAGT374620.17603176785572142No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA366710.17231490467773106No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTT364190.17113077127589338No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGC349670.1643079073891146No Hit
TCCAAGGCCTGAGTTACTACTGACGAGCAGT349150.16406356240143383No Hit
ACCCCTCCCCGCACATTGACCTCTT338740.15917196370574735No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG330990.15553028360088952No Hit
GTGCTAACGATTTGAAGTCTCTGGATTTT326430.15338756601661188No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC322620.15159726908764307No Hit
CGGACCTCGGATCAGCCT310800.146043119559976No Hit
GAGGTCCGGACCTGGGGAGGGTTATCCACT295080.1386563826247031No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCT292890.13762731431120134No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAT287290.13499590675156214No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGT271600.1276232666425016No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTA268130.12599273374393943No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCA261030.12265648487368257No Hit
TAGTAACAATGACCTGATCTGGAGACTCT258820.12161801867603923No Hit
AAAACTGCCGATTTTCCATGAA257820.12114812446896081No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTT256480.1205184662314757No Hit
TCACATCGAAAATCGTACATGGATTCACT255060.11985121645742434No Hit
CGGACCTCGGATCAGCCTG251590.11822068355886219No Hit
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGC237300.11150589533971142No Hit
TCTTTTATGTGCGGAACCTGTCAACCAAGT235470.1106459889407579No Hit
GAGGTCCGGACTTGGGGAGGGTTATC235200.11051911750484672No Hit
GAGGTCCGGACTTGGGGAGGGTTATCACCCT223140.10485219336748086No Hit
TAAGATTTAGGAAAATTTGCAGGCACTCCT218050.10246043185345163No Hit
TTCGCTACCGGCTCTATACTTTGGTCGTAT215880.10144076142409146No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)