FastQCFastQC Report
Wed 21 Jun 2023
sRNA-ACR-145-S1-TP2.flexbar_trim.20230621_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-145-S1-TP2.flexbar_trim.20230621_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19892972
Sequences flagged as poor quality0
Sequence length18-35
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT6790403.4134668263746617Illumina RNA PCR Primer (100% over 35bp)
GAGAATTCTACCACTGAACCACCAGTGC4898742.4625480797942108No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGC4569772.2971781189859413No Hit
TTCCGCCTCAGAAGAAAGATTTTCA3713011.8664933525267116No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGC1768840.8891783490169293No Hit
GAGAATTCTACCACTGAACCACAGTGC1742700.876038029913278No Hit
GGCGAGAATTCTACCACTGAACCACAGTGC1530710.7694727565091832No Hit
AGAATCATACCACTAGACCAAACAGCC1459600.7337264637983706No Hit
GCTTGTCAGTGGTAACTCAGGTCTTGGA1322530.6648227323700048No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGC1051100.5283775596728332No Hit
TTCCGCCCCAGAAGAAAGATTTTCA1006390.5059022854905743No Hit
ACAAGTTCGGATCTACGGGA1003680.5045399953310145No Hit
CCACAAGTTCGGATCTACGGGA987910.49661257252058666No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGC909810.45735247604028195No Hit
ACTGCTTGTCAGTGGTAACTCAGGTCTTGGA749450.3767410922812338No Hit
AGGGACGCTCGGACAGCCTTGCTCGCCTCGCAAGA718120.3609918115804918No Hit
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCC714060.3589508897916309No Hit
AGCGAGAATTCTACCACTGAACCACAGTGC629340.31636298487727227No Hit
TTTTTTTGGCCTTTAACCTACAGCATTAATA605420.30433863778624937No Hit
CTGCTTGTCAGTGGTAACTCAGGTCTTGGA462390.23243887338704342No Hit
GGAGTCTCCAGATCAGGTCATTGTTACTA447790.2250995979886766No Hit
GCGAGAATTCTACCACTGAACCACCAGTGC416130.2091844295563277No Hit
ACTTGTCAGTGGTAACTCAGGTCTTGGA389020.19555650105977126No Hit
TGCGAGAATTCTACCACTGAACCACAGTGC387040.19456117467012973No Hit
GCCGGATCCTAGCCACTAGACCATATGGGA377230.18962978483054216No Hit
GCTCGTCAGTGGTAACTCAGGTCTTGGA364530.1832456206141546No Hit
GAGTGGATAACCCTCCCCAAGTCCGGACCTC347360.17461443166963692No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG342080.17196022796392615No Hit
AAGAGGTCAATGTGCGGGGAGGGGTG341480.17165861390645903No Hit
AGGCGAGAATTCTACCACTGAACCACAGTGC300090.15085227084218486No Hit
TCACAAGTTCGGATCTACGGGA288770.1451618189579717No Hit
TGGCGAGAATTCTACCACTGAACCACCAGTGC285390.14346272643424018No Hit
AGTGGATAACCCTCCCCAAGTCCGGACCTC283600.1425629111627966No Hit
AACCCTTTCTTTGAAATACAGCGTCTTTTA273530.1375008218983066No Hit
ACTCTTCATTTATCTACTCGTTAA271760.13661106042877857No Hit
GCCCAGTTCAATTGTAAAAGACTACAGTCA268620.13503261352803392No Hit
TTTCATTTGTATCCTCACGGTTGACCCCT260790.13109655007808788No Hit
AGGGACTCTCGGACAGCCTTGCTCGCCTCGCAAGA258580.12998560496641728No Hit
GAGGGACTCTCGGACAGCCTTGCTCGCCTCGCAAG242240.1217716488013958No Hit
AGTGAATCCATGTACGATTTTCGATGTGA240310.12080145691654319No Hit
GGAATCATACCACTAGACCAAACAGCC226820.11402016752449055No Hit
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACC226620.11391962950533487No Hit
GAGGGACGCTCGGACAGCCTTGCTCGCCTCGCAAG226490.11385427979288362No Hit
AGTTTTGGCTTAACAAGTCTGGAGGCTCTA215740.10845036126326422No Hit
TGCTTGTCAGTGGTAACTCAGGTCTTGGA210170.1056503774297777No Hit
GAGAATCATACCACTAGACCAAACAGCC208910.10501698790909675No Hit
AGGCTGATCCGAGGTCCG204860.1029810930211936No Hit
GGGCGAGAATTCTACCACTGAACCACCAGTGC203180.10213657366028567No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)