FastQCFastQC Report
Wed 21 Jun 2023
sRNA-ACR-145-S1-TP2.flexbar_trim.20230621_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-145-S1-TP2.flexbar_trim.20230621_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19892972
Sequences flagged as poor quality0
Sequence length18-35
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCACACGTCTGAACTCCAGTCACCACCGG6360193.197204520269772TruSeq Adapter, Index 7 (96% over 31bp)
GCACTGGTGGTTCAGTGGTAGAATTCTC4955262.4909601240076142No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC4659062.342063317638008No Hit
TGAAAATCTTTCTTCTGAGGCGGAA3727901.873978408052854No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT1768210.8888616542565887No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC1760640.8850562902315451No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1557290.7828342592549772No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT1456840.7323390391340218No Hit
GAGACTCTGCACGGTGGATCTCTCGGCTCGCGCAT1360480.6838998215048008No Hit
TCCAAGACCTGAGTTACCACTGACAAGC1345860.6765504923045184No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA1048560.5271007268295557No Hit
TGAAAATCTTTCTTCTGGGGCGGAA1010600.508018610793802No Hit
TCCCGTAGATCCGAACTTGT1004530.5049672819124261No Hit
TCCCGTAGATCCGAACTTGTGG990670.4979999971849355No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCT912960.4589359498419844No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT749550.37679136129081164No Hit
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGC679680.3416684042987644No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT628330.3158552678805359No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA605810.30453468692360297No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG464800.23365035651786975No Hit
TAGTAACAATGACCTGATCTGGAGACTCC456250.22935235619896316No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC429090.21569929319761771No Hit
TCCAAGACCTGAGTTACCACTGACAAGT386880.19448074425480516No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA385750.1939127044465754No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGC384160.1931134271942875No Hit
TCCAAGACCTGAGTTACCACTGACGAGC372100.18705098463919823No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC351930.17691172540734487No Hit
CACCCCTCCCCGCACATTGACCTCTT345660.17375985850681336No Hit
AGTGATTGATAGGCGGGATGGATTCTTGAAAAAAA309170.15541669691185409No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT300970.15129463812647No Hit
TCCCGTAGATCCGAACTTGTGA289100.14532770668957862No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCA288640.14509646924552047No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT285070.14330186560359107No Hit
CGGACCTCGGATCAGCCTGGATCACCCGCCGAACT275830.13865700911859727No Hit
TAAAAGACGCTGTATTTCAAAGAAAGGGTT273600.1375360102050111No Hit
TGACTGTAGTCTTTTACAATTGAACTGGGC272520.13699310490157024No Hit
TTAACGAGTAGATAAATGAAGAGT271400.13643009199429829No Hit
AGGGGTCAACCGTGAGGATACAAATGAAA262590.13200139225048926No Hit
GGAAGAGCACACGTCGGAACTCCAGTCACCACCGG248490.12491346190001172TruSeq Adapter, Index 7 (96% over 31bp)
TCACATCGAAAATCGTACATGGATTCACT242200.12175154119756465No Hit
GGCTGTTTGGTCTAGTGGTATGATTCC228740.11498533250838537No Hit
TAGAGCCTCCAGACTTGTTAAGCCAAAACT216580.10887262094371822No Hit
AGACTCTGCACGGTGGATCTCTCGGCTCGCGCATC214390.10777172963396318No Hit
TCCAAGACCTGAGTTACCACTGACAAGCA213980.107565626694694No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTC210340.10573583474606006No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCC206720.10391609659934171No Hit
CGGACCTCGGATCAGCCT206540.10382561238210157No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)