FastQCFastQC Report
Mon 22 May 2023
sRNA-POC-57-S1-TP2_R2_001.fastp-trim.20230522.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-57-S1-TP2_R2_001.fastp-trim.20230522.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18888077
Sequences flagged as poor quality0
Sequence length15-150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTCAAGTCTCAGCCCGAG9435854.995664725424405No Hit
TTCCACTTCAGAGCAAAGATTTTCA6865263.6347056399653606No Hit
TGCTTCAAGTCTCAGCCCGAG4561342.4149308582340065No Hit
ATAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG2987741.5818126959139356No Hit
GGCCAGTCCGGGCCGGATCCAAC2368221.2538174214346967No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGC2044971.0826777125061486No Hit
AGAATTCTACCACTGAACCACCAGTGC1783840.9444264760250607No Hit
GAGAATTCTACCACTGAACCACCAGTGC1640400.8684843883260324No Hit
CGCCCCAACGACCGGCAGCAGTCG1602130.8482229292055512No Hit
GACCGCGACCTCGGAA1580940.8370042117045584No Hit
ACCCGAGGGTCACAGCTG1426230.7550953969533267No Hit
GCTTCAAGTCTCAGCCCGAG1325350.7016860424700725No Hit
TCCAACCGCTTCAAGTCTCAGCCCGAG1230080.6512468156498938No Hit
AACCGCTTCAAGTCTCAGCCCGAG1134530.600659347163822No Hit
CAGTTAAGAGCACACTC1095440.579963751736082No Hit
AGTTAAGAGCACACTC1017990.5389590480809666No Hit
TGCCCCAACGACCGGCAGCAGTCG930610.4926970596318514No Hit
TGCCAGTCCGGGCCGGATCCAAC918280.4861691319873378No Hit
TGACAGGGTCGAGTTCGACTATCTTGGTCG733740.3884672854732645No Hit
AGTTAAGAGCACACT688550.3645421394671358No Hit
AGCTGCTCCCACTTATTCTACACCTC677890.3588983674727713No Hit
AGCACTGCAAGCAGTCCACCACG639320.338478077995976No Hit
CAGTTAAGAGCACACT619350.32790527061066094No Hit
CCGCTTCAAGTCTCAGCCCGAG617140.3267352203191463No Hit
GGCGAGAATTCTACCACTGAACCACAGTGC611320.3236539114066509No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGC607650.32171088671440723No Hit
AGCCAGTCCGGGCCGGATCCAAC573840.30381070555779716No Hit
AGCCTTAACTCCAGAAAGTG563650.2984157677883249No Hit
AGAATTCTACCACTGAACCACAGTGC549930.29115192615955554No Hit
GAGAATTCTACCACTGAACCACAGTGC521170.2759253893342345No Hit
GGCCAATCCACGGCCGATTCCTTCC487010.25783990609525786No Hit
GCCTCGACCACGGCCAGTCCG470170.2489242287608209No Hit
GACAGGGTCGAGTTCGACTATCTTGGTCG460820.24397401598902843No Hit
CGGTCCGACCGCGACCTCGGAA459800.24343399277756014No Hit
GACCGCGACCTCGGAAAG455580.24119977909874044No Hit
ACCTCGACCACGGCCAGTCCG453820.24026797434169717No Hit
AGCCGGGCTGTAACAGTCCGGAGCCGAGACTCCAG436640.2311722892701041No Hit
ATCCAACCGCTTCAAGTCTCAGCCCGAG436560.23112993450842031No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGC398150.21079435455499257No Hit
GCCCCAACGACCGGCAGCAGTCG387860.20534647333341557No Hit
TAGTTAAGAGCACACTC377620.199925063837891No Hit
GGTCTCGGCGAGGAAGAACAGCTCGG377220.19971329002947202No Hit
AAACTAACCAGCCTAGACTTGA351050.18585798861366354No Hit
CGAGAATTCTACCACTGAACCACCAGTGC349120.1848361799880422No Hit
TCATGCTCTACCGACTGAGCTAGCCGGGC340070.1800447975725639No Hit
GACCCGAGGGTCACAGCTG335930.17785293865542798No Hit
ATCCAACCGCTTCAAGTCT322780.17089087470365563No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT322740.17086969732281376Illumina RNA PCR Primer (100% over 50bp)
GGAATTCTACCACTGAACCACCAGTGC321050.16997495298224377No Hit
GCCCGAGGGTCACAGCTG320240.16954611102019546No Hit
AGTCCGGGCCGGATCCAAC313230.1658347750276537No Hit
AGCCTTAACTCCAGAAAGTGA310390.1643311809878793No Hit
TGGTCCGACCGCGACCTCGGAA302900.1603657164252348No Hit
CGCGACCTCGGAAAG302410.16010629350992162No Hit
CGCTTCAAGTCTCAGCCCGAGA299710.15867682030309385No Hit
GGCCAATCCACGGCCGATTCCTTCCCGG298120.1578350194146286No Hit
GCCAGTCCGGGCCGGATCCAAC293430.15535197151091665No Hit
ATCCACGGCCGATTC270250.14307967931303964No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA269800.14284143377856837Illumina RNA PCR Primer (100% over 50bp)
CGCCCCAACGACCGGCAGCAGTC267220.14147549271426627No Hit
TCAAGTCTCAGCCCGAG263750.13963835492623203No Hit
TGCTGCTCCCACTTATTCTACACCTC253560.1342434171567598No Hit
TCATGCTCTACCGACTGAGCTAGCCGGG247250.1309026853289512No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGC245730.13009794485695925No Hit
GGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAG245290.1298649936676984No Hit
GGTCTCGGCGAGGAAGAACAGCTCGGC242360.12831375052102972No Hit
CAACCGCTTCAAGTCTCAGCCCGAG240640.12740312314482835No Hit
TACCGCGACCTCGGAA234160.12397238744844168No Hit
CGGTCTCGGCGAGGAAGAACAGCTCGG233960.1238665005442322No Hit
ACCCGAGGGTCACAGCT231920.12278645412129569No Hit
AGCTTCAAGTCTCAGCCCGAG231810.12272821632398045No Hit
AATCCACGGCCGATTC227320.12035105532447797No Hit
TGCCTTAACTCCAGAAAGTG223860.11851921188165424No Hit
TGCACTGCAAGCAGTCCACCACG220730.1168620818307761No Hit
CAGTTAAGAGCACACTCG220100.11652853808251629No Hit
ACAAGTTCGGATCTACGGGT219250.11607851873962605No Hit
TGCCAATCCACGGCCGATTCCTTCC217050.11491376279332195No Hit
TCAGTTAAGAGCACACT217030.114903174102901No Hit
GTAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG215850.11427844136806516No Hit
TAGTTAAGAGCACACT212890.11271131518576508No Hit
TGACAGGGTCGAGTTCGACTATCTTGGTCGG211050.111737155667038No Hit
GTCTCGGCGAGGAAGAACAGCTCGG210320.11135066846667345No Hit
GACCGCTTCAAGTCTCAGCCCGAG208370.11031827115063117No Hit
ACCGCTTCAAGTCTCAGCCCGAG207640.10993178395026662No Hit
ATCCACTTCAGAGCAAAGATTTTCA203440.10770815896186785No Hit
CGGAAACGTTCGAGTTGGCTATCGGTAGTG199920.10584454944778127No Hit
TGCGAGAATTCTACCACTGAACCACAGTGC199200.10546335659262719No Hit
AGTTAAGAGCACACTCG198130.10489686165510656No Hit
TCGCTTCAAGTCTCAGCCCGAG197340.10447860838347915No Hit
AGCCTTAACTCCAGAAAG195950.10374269439922339No Hit
GGTTAAGAGCACACTC195220.10335620719885882No Hit
GACAGGGTCGAGTTCGACTATCTTGGTCGG194820.1031444333904399No Hit
GACAGGGTCGAGTTCGACTATCTTGGTC192370.10184731881387396No Hit
TGACAGGGTCGAGTTCGA190090.10064020810588606No Hit
TCCAACCGCTTCAAG189410.10028019263157387No Hit
GTCGAGTTCGACTATCTTGGTC189040.10008430185878636No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)