FastQCFastQC Report
Mon 22 May 2023
sRNA-POC-57-S1-TP2_R1_001.fastp-trim.20230522.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-57-S1-TP2_R1_001.fastp-trim.20230522.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18888077
Sequences flagged as poor quality0
Sequence length15-150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGGCTGAGACTTGAAGCG9588855.076668207144644No Hit
TGAAAATCTTTGCTCTGAAGTGGAA7030753.7223217588534823No Hit
CTCGGGCTGAGACTTGAAGCA4679352.4774094260628017No Hit
AACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGCC2800301.482575489288825No Hit
GTTGGATCCGGCCCGGACTGGCC2435041.2891942361310789No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC2076771.0995137302754536No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT1820760.9639731985421279No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC1684250.8917000920739576No Hit
TTCCGAGGTCGCGGTC1633140.8646406937032287No Hit
CGACTGCTGCCGGTCGTTGGGGCG1626280.8610087728888441No Hit
CAGCTGTGACCCTCGGGT1480220.7836795667446719No Hit
CTCGGGCTGAGACTTGAAGC1371560.7261512116876695No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA1260050.6671139682456821No Hit
CTCGGGCTGAGACTTGAAGCGGTT1155800.6119204194264986No Hit
GAGTGTGCTCTTAACTG1110960.5881805755027365No Hit
GAGTGTGCTCTTAACT1036530.548774764101184No Hit
CGACTGCTGCCGGTCGTTGGGGCA942740.4991191003721554No Hit
GTTGGATCCGGCCCGGACTGGCA942610.49905027388441925No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCA762040.4034502824189037No Hit
AGTGTGCTCTTAACT702050.3716895055012747No Hit
GAGGTGTAGAATAAGTGGGAGCAGCT693350.36708342516816295No Hit
CGTGGTGGACTGCTTGCAGTGCT653990.3462448824197403No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC631590.33438554914828017No Hit
AGTGTGCTCTTAACTG628620.33281312862076956No Hit
CTCGGGCTGAGACTTGAAGCGG627320.3321248637434081No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA604770.3201861152937909No Hit
GTTGGATCCGGCCCGGACTGGCT585730.31010568201304983No Hit
CACTTTCTGGAGTTAAGGCT574570.30419719275816165No Hit
GCACTGTGGTTCAGTGGTAGAATTCT560710.29685923029644573No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGCCGTCTTCTGCTT553210.2928884713885908RNA PCR Primer, Index 28 (100% over 42bp)
GCACTGTGGTTCAGTGGTAGAATTCTC533780.28260155864464126No Hit
GGAAGGAATCGGCCGTGGATTGGCC513070.2716369697137512No Hit
CGGACTGGCCGTGGTCGAGGC484620.25657455758995473No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC481930.25515037872833746No Hit
TTCCGAGGTCGCGGTCGGACCG471560.2496601427450767No Hit
CTTTCCGAGGTCGCGGTC469710.24868068888113914No Hit
CGGACTGGCCGTGGTCGAGGT463970.2456417347303275No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGAT446280.23627603805300032No Hit
CTGGAGTCTCGGCTCCGGACTGTTACAGCCCGGCT436120.23089698331915948No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT396300.209814900691055No Hit
CGACTGCTGCCGGTCGTTGGGGC395850.2095766551565837No Hit
CCGAGCTGTTCTTCCTCGCCGAGACC391710.20738479623944778No Hit
GAGTGTGCTCTTAACTA389200.20605591559161895No Hit
CAACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGC386170.20445172899284558No Hit
TCAAGTCTAGGCTGGTTAGTTT358050.1895640302609948No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCG356760.18888105972884375No Hit
CAGCTGTGACCCTCGGGTC350960.18581033950676928No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA344950.18262843803527484No Hit
CAGCTGTGACCCTCGGGC338230.17907063805383683No Hit
GCACTGGTGGTTCAGTGGTAGAATTCC334090.1768787791367009No Hit
AGACTTGAAGCGGTTGGAT329850.17463397676746023No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCC324440.1717697360085942No Hit
GTTGGATCCGGCCCGGACT318540.16864607233441498No Hit
TCACTTTCTGGAGTTAAGGCT316080.16734366341263857No Hit
TTCCGAGGTCGCGGTCGGACCA314450.16648068514333142No Hit
CTTTCCGAGGTCGCG310140.16419882235761746No Hit
GTTGGATCCGGCCCGGACTGGC304290.1611016304094906No Hit
TCTCGGGCTGAGACTTGAAGCG304180.1610433926121754No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCGCGTATGCCGTCTTCTGCTT287140.15202182837352896RNA PCR Primer, Index 28 (97% over 42bp)
GAATCGGCCGTGGAT275190.14569508584701343No Hit
GACTGCTGCCGGTCGTTGGGGCG271300.14363558556013933No Hit
CTCGGGCTGAGACTTGA268920.1423755314000467No Hit
GAGGTGTAGAATAAGTGGGAGCAGCA262680.13907185998871138No Hit
CCCGGCTAGCTCAGTCGGTAGAGCATGA254980.13499521417664698No Hit
GCCGAGCTGTTCTTCCTCGCCGAGACC250850.13280864960472155No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCC249020.13183978443120492No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCT246160.1303256017010096No Hit
CTCGGGCTGAGACTTGAAGCGGTTG245550.1300026466431707No Hit
TTCCGAGGTCGCGGTA239940.1270325189800952No Hit
CCGAGCTGTTCTTCCTCGCCGAGACCG238580.12631248803147085No Hit
CTCGGGCTGAGACTTGAAGCT236110.12500478476448398No Hit
AGCTGTGACCCTCGGGT235690.12478242226564408No Hit
CACTTTCTGGAGTTAAGGCA230370.12196583061367232No Hit
GAATCGGCCGTGGATT228920.1211981505581537No Hit
CGTGGTGGACTGCTTGCAGTGCA228350.12089637288115673No Hit
ACCCGTAGATCCGAACTTGT225390.11932924669885664No Hit
CGAGTGTGCTCTTAACTG223720.1184450910487076No Hit
AGTGTGCTCTTAACTGA223490.11832332110886673No Hit
AGTGTGCTCTTAACTA219690.11631146992888688No Hit
CCGAGCTGTTCTTCCTCGCCGAGAC218400.11562849939673583No Hit
GGAAGGAATCGGCCGTGGATTGGCA217890.1153584877910017No Hit
CTCGGGCTGAGACTTGAAGCGGTC215760.1142307922611709No Hit
CTCGGGCTGAGACTTGAAGCGGT212900.11271660953097555No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTCA210890.11165244614367043No Hit
CTCGGGCTGAGACTTGAAGCGA208760.11052475061383961No Hit
CACTACCGATAGCCAACTCGAACGTTTCCG208060.1101541464491065No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA204760.1084070125296503No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTC203170.10756521164118507No Hit
TGAAAATCTTTGCTCTGAAGTGGAT202900.1074222643205023No Hit
GAGTGTGCTCTTAACC202230.10706754319140059No Hit
CGAGTGTGCTCTTAACT201980.10693518456113876No Hit
GACCAAGATAGTCGAACTCGACCCTGTC200120.10595043635199072No Hit
CTTTCTGGAGTTAAGGCT200010.10589219855467553No Hit
GACCAAGATAGTCGAACTCGAC196470.104018000350168No Hit
TCGAACTCGACCCTGTCA194490.10296971999849429No Hit
CTTGAAGCGGTTGGA193930.10267323666670779No Hit
CGGACTGGCCGTGGTCGAGG193300.10233969291844798No Hit
GACTTGAAGCGGTTGGAT189150.10014253965610158No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)