FastQCFastQC Report
Mon 22 May 2023
sRNA-POC-53-S1-TP2_R2_001.fastp-trim.20230522.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-53-S1-TP2_R2_001.fastp-trim.20230522.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19550447
Sequences flagged as poor quality0
Sequence length15-150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTCAAGTCTCAGCCCGAG15964498.165792833278953No Hit
TTCCACTTCAGAGCAAAGATTTTCA9168484.689652364470234No Hit
TTCCACCTCAGAGCAAAGATTTTCA5375582.749594421038046No Hit
TGCTTCAAGTCTCAGCCCGAG4729212.4189779394813837No Hit
ATAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG4112122.103338097589278No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGC1670310.8543589821757017No Hit
GGCCAGTCCGGGCCGGATCCAAC1298100.6639745884071091No Hit
AACCGCTTCAAGTCTCAGCCCGAG1006390.5147657237709194No Hit
GCTTCAAGTCTCAGCCCGAG1000490.5117478899587308No Hit
CGCTTCAAGTCTCAGCCCGAGA987950.5053337143646894No Hit
GCCTCGACCACGGCCAGTCCG979080.5007967337012806No Hit
AGAATTCTACCACTGAACCACCAGTGC966550.4943876730798022No Hit
CGCGACCTCGGAAAG870170.4450895675173054No Hit
GAGAATTCTACCACTGAACCACCAGTGC859960.43986718053045026No Hit
CTCCACTTCAGAGCAAAGATTTTCA813410.416056983249539No Hit
CGCCCCAACGACCGGCAGCAGTCG774300.3960523255555231No Hit
CCGCTTCAAGTCTCAGCCCGAG733870.3753724914831871No Hit
CCACAAGTTCGGATCTACGGGT697280.3566568068750551No Hit
CGAGAATTCTACCACTGAACCACCAGTGC622810.31856560619816005No Hit
AAACTAACCAGCCTAGACTTGA562520.28772743661564365No Hit
CGTTTCAAGTCTCAGCCCGAG543960.27823404753865727No Hit
CTCCACCTCAGAGCAAAGATTTTCA528500.2703262999562107No Hit
TGCCAGTCCGGGCCGGATCCAAC512710.2622497582791841No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGC497490.25446477003825024No Hit
TGACAGGGTCGAGTTCGACTATCTTGGTCG496090.25374867387942585No Hit
GGCGAGAATTCTACCACTGAACCACAGTGC483750.24743679773664512No Hit
TCCACAGAATTATTTAAAAGTGATGCAAGA482650.24687415075471164No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT471050.24094078258159518Illumina RNA PCR Primer (100% over 50bp)
CAGTTAAGAGCACACTC461400.23600483405826989No Hit
ACAAGTTCGGATCTACGGGT439740.2249258034867438No Hit
TCCAACCGCTTCAAGTCTCAGCCCGAG416440.2130079174148806No Hit
GACCGCGACCTCGGAAAG387630.19827168146078705No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA374970.19179612619598926Illumina RNA PCR Primer (100% over 50bp)
GGCCAATCCACGGCCGATTCCTTCC366500.18746374443510166No Hit
CGGTCTCGGCGAGGAAGAACAGCTCGG361880.18510062711098116No Hit
CCAGAATTGCCTGAAGCCGTGCAGGAA343360.17562769792424693No Hit
ACCCGAGGGTCACAGCTG334180.1709321531113841No Hit
GTAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG334070.17087588841319076No Hit
AGTTAAGAGCACACTC299840.15336733732993418No Hit
GACAGGGTCGAGTTCGACTATCTTGGTCG296430.1516231316859405No Hit
TGCTTCAAGTCTCAGCCCGAGA296230.15152083223467985No Hit
GCCAGAATTGCCTGAAGCCGTGCAGGAA294570.15067174678921663No Hit
TCATGCTCTACCGACTGAGCTAGCCGGGC291020.14885593152934048No Hit
AGAATTCTACCACTGAACCACAGTGC288340.14748511888244806No Hit
CAACCGCTTCAAGTCTCAGCCCGAG288120.14737258948606138No Hit
TGCCCCAACGACCGGCAGCAGTCG286110.14634448000089206No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGC282950.1447281486709741No Hit
AGCCAGTCCGGGCCGGATCCAAC279360.14289187352084584No Hit
TGCGACCTCGGAAAG275030.14067709040105322No Hit
CCACTTCAGAGCAAAGATTTTCA264300.1351887248409205No Hit
GGTCTCGGCGAGGAAGAACAGCTCGG262690.13436521425827247No Hit
CCACCTCAGAGCAAAGATTTTCA261940.1339815913160451No Hit
GAGAATTCTACCACTGAACCACAGTGC261900.13396113142579297No Hit
CGGAAACGTTCGAGTTGGCTATCGGTAGTG261730.13387417689222145No Hit
TCCGGGCCGGATCCAAC260430.13320923045902736No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGC242250.12391021033943622No Hit
GGCCAATCCACGGCCGATTCCTTCCCGG231630.11847810947749687No Hit
CGGTCCGACCGCGACCTCGGAAAGGG227280.1162530964125782No Hit
AGCTGCTCCCACTTATTCTACACCTC220610.11284140971303622No Hit
GCCAGTCCGGGCCGGATCCAAC214020.10947064279399853No Hit
ATCCACTTCAGAGCAAAGATTTTCA212170.10852437286983771No Hit
GCCAGTCCGGGCCGGATCC210380.10760879278105509No Hit
TCAAGTCTCAGCCCGAG208790.1067955121435331No Hit
AGATTAAGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGC207990.10638631433849056No Hit
TTTTTTTGGTCTTTCACCTACAGCATTAATA206960.10585947216449833No Hit
TTCCCGGTCCGACCGCGACCTCGGAAAG204420.10456026913348836No Hit
ATCCAACCGCTTCAAGTCTCAGCCCGAG204090.1043914750389083No Hit
GACCGCGACCTCGGAAAGGG202130.10338894041655416No Hit
GACCGCTTCAAGTCTCAGCCCGAG201510.1030718121176462No Hit
AGCCGGGCTGTAACAGTCCGGAGCCGAGACTCCAG200600.10260634961441034No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)