FastQCFastQC Report
Mon 22 May 2023
sRNA-POC-53-S1-TP2_R1_001.fastp-trim.20230522.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-53-S1-TP2_R1_001.fastp-trim.20230522.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19550447
Sequences flagged as poor quality0
Sequence length15-150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGGCTGAGACTTGAAGCG16327228.351328233057792No Hit
TGAAAATCTTTGCTCTGAAGTGGAA9394484.805250744394744No Hit
TGAAAATCTTTGCTCTGAGGTGGAA5514752.820779494197754No Hit
CTCGGGCTGAGACTTGAAGCA4872792.492418715541389No Hit
AACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGCC4000952.0464749476060575No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC1690160.864512202713319No Hit
GTTGGATCCGGCCCGGACTGGCC1343430.6871607590353306No Hit
CTCGGGCTGAGACTTGAAGC1044490.5342537692360692No Hit
CTCGGGCTGAGACTTGAAGCGGTT1016240.5198039717455053No Hit
CGGACTGGCCGTGGTCGAGGC1013290.5182950548394111No Hit
TCTCGGGCTGAGACTTGAAGCG1008910.5160546968568034No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT990110.5064385484383043No Hit
CTTTCCGAGGTCGCG889150.454797785441939No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC886150.4532632936730296No Hit
CGACTGCTGCCGGTCGTTGGGGCG766500.3920626469563586No Hit
CTCGGGCTGAGACTTGAAGCGG752610.38495795006630795No Hit
TGAAAATCTTTGCTCTGAAGTGGAG732240.37453875095541295No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCG640200.3274605434852717No Hit
TCAAGTCTAGGCTGGTTAGTTT570470.2917938398032536No Hit
CAACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGC570310.29171200024224514No Hit
CTCGGGCTGAGACTTGAAACG556760.2847812124193375No Hit
ACCCGTAGATCCGAACTTGTGG546740.27965600991118006No Hit
GTTGGATCCGGCCCGGACTGGCA527300.26971250324864693No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCA517560.2647305199722544No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATTCCTATCTCGTATGCCGTCTTCTGCTT517550.2647254049996913TruSeq Adapter, Index 27 (98% over 58bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCC501450.2564902991732107No Hit
TGAAAATCTTTGCTCTGAGGTGGAG496540.2539788476447623No Hit
TCTTGCATCACTTTTAAATAATTCTGTGGA496160.2537844786873671No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA494730.25305303761085357No Hit
ACCCGTAGATCCGAACTTGT438500.2242915468889279No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA428310.2190793898471989No Hit
CTTTCCGAGGTCGCGGTC401170.20519735431113162No Hit
GGAAGGAATCGGCCGTGGATTGGCC390010.1994890449307885No Hit
CCGAGCTGTTCTTCCTCGCCGAGACCG375180.19190354061981293No Hit
CAGCTGTGACCCTCGGGT348240.17812380453500629No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATTCCTATCGCGTATGCCGTCTTCTGCTT344220.17606758556466764RNA PCR Primer, Index 27 (97% over 42bp)
CGACCAAGATAGTCGAACTCGACCCTGTC310780.15896311731389057No Hit
GAGTGTGCTCTTAACT306760.15690689834355195No Hit
TTCCTGCACGGCTTCAGGCAATTCTGGC304830.15591970863888688No Hit
TCTCGGGCTGAGACTTGAAGCA304560.15578160437968502No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA296410.1516129017408144No Hit
GCACTGTGGTTCAGTGGTAGAATTCT294730.15075358635022515No Hit
GTTGGATCCGGCCCGGACTGGCT286100.14633936502832903No Hit
CTTTCCGAGGTCGCA284340.1454391298572355No Hit
CGACTGCTGCCGGTCGTTGGGGCA283470.14499412724425176No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT281300.14388417819807395No Hit
CACTACCGATAGCCAACTCGAACGTTTCCG273540.13991495948916155No Hit
CCGAGCTGTTCTTCCTCGCCGAGACC273310.13979731512021185No Hit
GAGTGTGCTCTTAACGG272540.1394034622328584No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC269680.13794058007983143No Hit
GTTGGATCCGGCCCGGA266150.13613499476508134No Hit
TTCCTGCACGGCTTCAGGCAATTCTGG263360.13470791741999555No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCC252180.12898937809452643No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCT242090.12382837077842773No Hit
CCCTTTCCGAGGTCGCGGTCGGACCG236130.120779847130861No Hit
TGAAAATCTTTGCTCTGAAGTGG234860.120130245615356No Hit
TGAAAATCTTTGCTCTGAGGTGG228670.11696407759883955No Hit
GAGGTGTAGAATAAGTGGGAGCAGCT226650.1159308531411072No Hit
GTTGGATCCGGCCCGGACTGGC223230.11418153252455046No Hit
GGATCCGGCCCGGACTGGC217630.11131714788925287No Hit
CTCGGGCTGAGACTTGA214480.10970593153189798No Hit
TGAAAATCTTTGCTCTGAAGTGGAT210320.10757810294567689No Hit
CCCTTTCCGAGGTCGCGGTC209770.10729677945471017No Hit
CTCGGGCTGAGACTTGAAGCGGTC209630.10722516983882772No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA209130.10696942121067615No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGAT207890.10633516461286026No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA207390.10607941598470869No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTTAATCT206940.10584924221937228No Hit
GGATCCGGCCCGGACTGGCC201070.10284675332487284No Hit
CTGGAGTCTCGGCTCCGGACTGTTACAGCCCGGCT200670.10264215442235157No Hit
GAGTGTGCTCTTAACTG200580.1025961196692843No Hit
CAGCTGTGACCCTCGGG200010.1023045662331915No Hit
TGAAAATCTTTGCTCTGAAGTGGA199930.10226364645268725No Hit
TGAAAATCTTTGCTCTGAGGTGGA197520.10103093806499666No Hit
GCACTGGTGGTTCAGTGGTAGAATTCC195690.10009489808596192No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)