FastQCFastQC Report
Mon 22 May 2023
sRNA-POC-50-S1-TP2_R2_001.fastp-trim.20230522.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-50-S1-TP2_R2_001.fastp-trim.20230522.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15262654
Sequences flagged as poor quality0
Sequence length15-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCA301412819.748387141581013No Hit
ATAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG6404224.19600680196249No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGC3375452.211574736608718No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGC1256790.8234413228525No Hit
GAGAATTCTACCACTGAACCACCAGTGC1202430.7878249746079548No Hit
CGCTTCAAGTCTCAGCCCGAG1153240.755595979572098No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT1041770.682561499461365Illumina RNA PCR Primer (100% over 50bp)
AAACTAACCAGCCTAGACTTGA959360.6285669582760639No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA868470.5690163715956609Illumina RNA PCR Primer (100% over 50bp)
TGCTTCAAGTCTCAGCCCGAG844910.5535799999135144No Hit
GGCGAGAATTCTACCACTGAACCACAGTGC831350.544695568673705No Hit
ACAAGTTCGGATCTACGGGT724480.47467498116644724No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGC641410.4202480119119519No Hit
TCCACTTCAGAGCAAAGATTTTCA630970.41340778609015183No Hit
ATCCACTTCAGAGCAAAGATTTTCA616290.40378953752080077No Hit
GTAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG564610.369929109314802No Hit
CCACAAGTTCGGATCTACGGGT547920.3589939207165412No Hit
GCCTCGACCACGGCCAGTCCG513690.33656662858242087No Hit
GCCAGAATTGCCTGAAGCCGTGCAGGAA468110.3067028840462478No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA446870.29278656254672353Illumina RNA PCR Primer (100% over 50bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAA433190.2838235080216062Illumina RNA PCR Primer (100% over 50bp)
AACCGCTTCAAGTCTCAGCCCGAG430800.2822575942558876No Hit
CTCCACTTCAGAGCAAAGATTTTCA428660.2808554790012274No Hit
AGAATTCTACCACTGAACCACCAGTGC407780.2671750273576273No Hit
GGCCAGTCCGGGCCGGATCCAAC365820.23968308526158033No Hit
TCCAACCGCTTCAAGTCTCAGCCCGAG361190.23664953683677817No Hit
CCACTTCAGAGCAAAGATTTTCA357880.23448084455036455No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGC333390.21843514240708073No Hit
TGCGAGAATTCTACCACTGAACCACAGTGC333060.21821892837248358No Hit
GAGAATTCTACCACTGAACCACAGTGC330620.21662025490455328No Hit
TTTTTTTGGTCTTTCACCTACAGCATTAATA307790.20166217487469743No Hit
CCAGAATTGCCTGAAGCCGTGCAGGAA286160.187490327697922No Hit
TCCCACTTCAGAGCAAAGATTTTCA265130.1737115969476868No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAT237340.15550375445843168Illumina RNA PCR Primer (100% over 50bp)
TTCCACTTCAGAGTAAAGATTTTCA216200.14165295236333078No Hit
TCACAAGTTCGGATCTACGGGT210390.13784627496633287No Hit
TGCCAGTCCGGGCCGGATCCAAC207850.1361820820939792No Hit
TTCCACTTCAGAGCAAAGATTTTCG198820.13026567987454868No Hit
TGGCGAGAATTCTACCACTGAACCACCAGTGC192570.12617071709808791No Hit
TCATGCTCTACCGACTGAGCTAGCCGGGC179010.11728628585827865No Hit
GAACTAACCAGCCTAGACTTGA170380.11163196125654164No Hit
GCAAGTTCGGATCTACGGGT169800.11125194871088606No Hit
TGTCTTTCACCTACAGCATTAATA162780.10665248652036534No Hit
AGCGAGAATTCTACCACTGAACCACAGTGC161840.10603660411878564No Hit
TCACTTCAGAGCAAAGATTTTCA161760.10598418859524693No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAC160880.10540761783632126Illumina RNA PCR Primer (100% over 50bp)
AATGAGGTCAAAGTAGCGGATGAGGGGTG155260.10172542730772774No Hit
ACTCATCCTAAGTAGACTTTTCACATTGAA154560.10126679147676412No Hit
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGACTGTAGAACTCTGAAC154360.10113575266791738No Hit
GTCGTCAGCGTTCAAATATGGAAACAGA152760.10008744219714343No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)