FastQCFastQC Report
Mon 22 May 2023
sRNA-POC-50-S1-TP2_R1_001.fastp-trim.20230522.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-50-S1-TP2_R1_001.fastp-trim.20230522.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15262654
Sequences flagged as poor quality0
Sequence length15-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA308411320.206924693437983No Hit
AACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGCC6068133.9758026356359775No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC3410652.2346375669657452No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA1251620.8200539696438117No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC1229270.805410382755188No Hit
CTCGGGCTGAGACTTGAAGCG1172520.7682281207449242No Hit
TCAAGTCTAGGCTGGTTAGTTT976370.6397118089684796No Hit
CAACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGC919590.6025098911368888No Hit
CTCGGGCTGAGACTTGAAGCA868200.5688394692037178No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC854540.5598895185594852No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATGCCGTCTTCTGCTT783570.5133903972402178RNA PCR Primer, Index 26 (100% over 42bp)
ACCCGTAGATCCGAACTTGT743320.4870188369598106No Hit
TGAAAATCTTTGCTCTGAAGTGGA650380.42612510248872837No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT638140.41810552738730766No Hit
TGAAAATCTTTGCTCTGAAGTGGAT610890.40025148968193863No Hit
ACCCGTAGATCCGAACTTGTGG575920.3773393539550854No Hit
CGGACTGGCCGTGGTCGAGGC528680.34638798730548437No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCGCGTATGCCGTCTTCTGCTT513540.3364683494757858RNA PCR Primer, Index 26 (97% over 42bp)
TTCCTGCACGGCTTCAGGCAATTCTGGC483660.31689115143408214No Hit
TGAAAATCTTTGCTCTGAAGTGGAG442100.2896612869557287No Hit
CTCGGGCTGAGACTTGAAGCGGTT438630.2873877636222376No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT415850.27246244329459346No Hit
GTTGGATCCGGCCCGGACTGGCC373250.24455117701023688No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA370870.24299181518496063No Hit
TGAAAATCTTTGCTCTGAAGTGG362970.2378157822355142No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA343520.22507225807516829No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC336720.22061693857437903No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCT333470.21848755793061939No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA308380.20204873936079532No Hit
TTCCTGCACGGCTTCAGGCAATTCTGG291980.19130355703536225No Hit
TGAAAATCTTTGCTCTGAAGTGGGA273530.1792152269192501No Hit
ACCCGTAGATCCGAACTTGTGA223870.1466782906826034No Hit
TGAAAATCTTTACTCTGAAGTGGAA221360.1450337536315768No Hit
GTTGGATCCGGCCCGGACTGGCA214090.14027049292999763No Hit
CGAAAATCTTTGCTCTGAAGTGGAA200680.13148434079682342No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCA192210.12593484724216378No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA180990.11858357006586141No Hit
ACCCGTAGATCCGAACTTGC177560.11633625449413974No Hit
TCAAGTCTAGGCTGGTTAGTTC176390.1155696774623863No Hit
TATTAATGCTGTAGGTGAAAGACA166570.10913567194801115No Hit
TGAAAATCTTTGCTCTGAAGTGA166380.10901118507960672No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT164520.10779252415733201No Hit
CACCCCTCATCCGCTACTTTGACCTCATT161890.10606936382099731No Hit
TTCAATGTGAAAAGTCTACTTAGGATGAGT157800.1033896201800814No Hit
TCTGTTTCCATATTTGAACGCTGACGAC157750.10335686047786971No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)