FastQCFastQC Report
Mon 22 May 2023
sRNA-POC-48-S1-TP2_R2_001.fastp-trim.20230522.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-48-S1-TP2_R2_001.fastp-trim.20230522.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17319013
Sequences flagged as poor quality0
Sequence length15-150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCA310891017.950849739531925No Hit
GAGAATTCTACCACTGAACCACCAGTGC9765795.638768213869924No Hit
ATAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG5549113.2040567207842616No Hit
GAGAATTCTACCACTGAACCACAGTGC2338761.3504002797388048No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGC1859311.0735657973118908No Hit
AGAATTCTACCACTGAACCACCAGTGC1645560.9501465239387488No Hit
CGCTTCAAGTCTCAGCCCGAG1387150.8009405616821236No Hit
GACCGCGACCTCGGAAAGGG1157500.6683406265703479No Hit
GACCGCGACCTCGGAAAGGGA1142030.6594082468787338No Hit
GACCGCGACCTCGGAAAG1109660.6407178053391379No Hit
TGCTTCAAGTCTCAGCCCGAG935310.5400480962743085No Hit
GACCGCGACCTCGGAAAGGGAG931490.5378424278565991No Hit
GGCCAGTCCGGGCCGGATCC851940.491910249157963No Hit
AACCGCTTCAAGTCTCAGCCCGAG804770.46467428600001626No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT793070.4579187047206443Illumina RNA PCR Primer (100% over 50bp)
GGCCAGTCCGGGCCGGATCCAAC762050.44000775332866837No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA728450.42060710965457443Illumina RNA PCR Primer (100% over 50bp)
TGACAGGGTCGAGTTCGACTATCTTGGTCG724090.41808964517781705No Hit
AAACTAACCAGCCTAGACTTGA694410.40095240993236736No Hit
TCCACTTCAGAGCAAAGATTTTCA680760.3930708984397667No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGC678760.39191609822107065No Hit
ATCCACTTCAGAGCAAAGATTTTCA581000.335469463531207No Hit
ACAAGTTCGGATCTACGGGT556240.3211730368237497No Hit
GACCGCGACCTCGGAAAGG530910.30654749205396403No Hit
GCCAGTCCGGGCCGGATCC506030.29218177733338496No Hit
AACCGCTTCAAGTCTCAGCCCGAGA491970.2840635317959517No Hit
CGCTTCAAGTCTCAGCCCGAGA483070.2789246708227542No Hit
GTAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG452830.26146409151606964No Hit
GGCGAGAATTCTACCACTGAACCACAGTGC440820.2545295162027998No Hit
TGCCAGTCCGGGCCGGATCC416250.24034279551611865No Hit
CCACAAGTTCGGATCTACGGGT415710.24003099945707068No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA391610.22611565682178308Illumina RNA PCR Primer (100% over 50bp)
TGCCAGTCCGGGCCGGATCCAAC390840.22567105873758508No Hit
ACTCGTTCTCAAAGTAGTTCAGCAGAA369760.21349946443252857No Hit
AGAATTCTACCACTGAACCACAGTGC368880.21299135233630231No Hit
CTCCACTTCAGAGCAAAGATTTTCA356730.20597594100772368No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGC340090.19636800318817244No Hit
CAGTTAAGAGCACACTC339770.1961832351531811No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAA338010.19516701096072855Illumina RNA PCR Primer (100% over 50bp)
CCACTTCAGAGCAAAGATTTTCA329200.19008011599737237No Hit
TGCTTCAAGTCTCAGCCCGAGA326810.18870012973603056No Hit
GAGAATTCTACCACTGAACCACAAGTGC305450.1763668634003566No Hit
GACCGCGACCTCGGAAA303680.17534486520681059No Hit
CAGTTAAGAGCACACT295550.17065060231781107No Hit
GGAATTCTACCACTGAACCACCAGTGC292480.16887798398211262No Hit
TCCCACTTCAGAGCAAAGATTTTCA260480.15040118048297554No Hit
TTCCACCTCAGAGCAAAGATTTTCA254100.14671736778533512No Hit
GCGAGAATTCTACCACTGAACCACCAGTGC251010.1449332014474497No Hit
GCTTCAAGTCTCAGCCCGAG241860.1396499904469152No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGC223290.12892767041632222No Hit
GCCTCGACCACGGCCAGTCCG219920.12698183204781935No Hit
TACCGCGACCTCGGAAAGGGA218840.12635823992972348No Hit
TACCGCGACCTCGGAAAG215700.12454520358637065No Hit
TACCGCGACCTCGGAAAGGG215630.12450478557871629No Hit
CGGAAACGTTCGAGTTGGCTATCGGTAGTG215170.1242391815284162No Hit
TTCCACTTCAGAGCAAAGATTTTCG195820.11306648941253176No Hit
AGCCAGTCCGGGCCGGATCC194040.11203871721789227No Hit
GAGAATTCTACCACTGAACCACCAGTGT191760.11072224496857874No Hit
AGTCCGGGCCGGATCCAAC191560.11060676494670915No Hit
TCATGCTCTACCGACTGAGCTAGCCGGGC188650.10892653062850638No Hit
TTCCCGGTCCGACCGCGACCTCGGAAAG188610.10890343462413245No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAT183950.10621275011457063Illumina RNA PCR Primer (100% over 50bp)
TACCGCGACCTCGGAAAGGGAG182800.10554873998882039No Hit
GGCCAGTCCGGGCCGGATCCA180620.10429000775044167No Hit
GTCGAGTTCGACTATCTTGGTC176910.10214785334476045No Hit
TGCGAGAATTCTACCACTGAACCACAGTGC175090.10109698514574705No Hit
GCCAGAATTGCCTGAAGCCGTGCAGGAA173510.10018469297297715No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)