FastQCFastQC Report
Mon 22 May 2023
sRNA-POC-48-S1-TP2_R1_001.fastp-trim.20230522.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-48-S1-TP2_R1_001.fastp-trim.20230522.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17319013
Sequences flagged as poor quality0
Sequence length15-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA318372918.382854727345027No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC9995155.771200702949989No Hit
AACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGCC5287483.052991530175536No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC2388681.3792240931974589No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC1885831.0888784482118006No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT1677100.9683577233875856No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACACTGATATCTCGTATGCCGTCTTCTGCTT1479900.8544944218241537TruSeq Adapter, Index 25 (100% over 58bp)
CTCGGGCTGAGACTTGAAGCG1413510.8161608285645378No Hit
CCCTTTCCGAGGTCGCGGTC1195860.6904896947649384No Hit
TCCCTTTCCGAGGTCGCGGTC1179510.681049202977098No Hit
CTTTCCGAGGTCGCGGTC1146300.6618737453456499No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACACTGATATCGCGTATGCCGTCTTCTGCTT1005690.580685515970223TruSeq Adapter, Index 25 (98% over 58bp)
CTCCCTTTCCGAGGTCGCGGTC963860.5565328693961948No Hit
CTCGGGCTGAGACTTGAAGCA963150.5561229153185577No Hit
GGATCCGGCCCGGACTGGCC867650.5009812048758205No Hit
CTCGGGCTGAGACTTGAAGCGGTT822350.47482497992235473No Hit
GTTGGATCCGGCCCGGACTGGCC778040.4492403810771433No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCA752530.434510904287675No Hit
CAACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGC720530.41603410078853803No Hit
TCAAGTCTAGGCTGGTTAGTTT709980.40994252963491623No Hit
TGAAAATCTTTGCTCTGAAGTGGA700280.4043417485742403No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA676370.39053611195972887No Hit
TGAAAATCTTTGCTCTGAAGTGGAT574530.33173368482372523No Hit
ACCCGTAGATCCGAACTTGT571680.3300880945120834No Hit
CCTTTCCGAGGTCGCGGTC546760.31569928378713036No Hit
GGATCCGGCCCGGACTGGC524080.302603849307117No Hit
TCTCGGGCTGAGACTTGAAGCGGTT500380.2889194667155686No Hit
TCTCGGGCTGAGACTTGAAGCG492710.2844908078768692No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC454740.2625669257249244No Hit
ACCCGTAGATCCGAACTTGTGG439720.25389437608251697No Hit
GGATCCGGCCCGGACTGGCA429170.2478028049288952No Hit
GTTGGATCCGGCCCGGACTGGCA402240.2322534199841527No Hit
TTCTGCTGAACTACTTTGAGAACGAGT378370.21847087937401513No Hit
GCACTGTGGTTCAGTGGTAGAATTCT375980.21709089311267335No Hit
TGAAAATCTTTGCTCTGAAGTGGAG368270.2126391382696No Hit
GAGTGTGCTCTTAACTG345060.19923768173163214No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT338170.19525939497822423No Hit
TGAAAATCTTTGCTCTGAAGTGG336400.1942373967846782No Hit
TCTCGGGCTGAGACTTGAAGCA334590.19319230258675826No Hit
TTTCCGAGGTCGCGGTC314330.1814941763713671No Hit
GCACTTGTGGTTCAGTGGTAGAATTCTC312160.1802412181340819No Hit
GCACTGGTGGTTCAGTGGTAGAATTCC303580.17528712519587578No Hit
AGTGTGCTCTTAACTG300540.17353182886345775No Hit
TGAAAATCTTTGCTCTGAAGTGGGA269360.1555284934539861No Hit
TGAAAATCTTTGCTCTGAGGTGGAA262060.15131347265574546No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC261010.15070720254093004No Hit
CTCGGGCTGAGACTTGAAGC250800.1448119474244866No Hit
CGGACTGGCCGTGGTCGAGGC227060.1311044688285643No Hit
CACTACCGATAGCCAACTCGAACGTTTCCG224950.12988615459783995No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCT223540.12907202044365923No Hit
TCCCTTTCCGAGGTCGCGGTA222670.12856968234852645No Hit
CCCTTTCCGAGGTCGCGGTA220570.12735714211889557No Hit
CTTTCCGAGGTCGCGGTA219480.12672777599970622No Hit
ACACTGGTGGTTCAGTGGTAGAATTCTC199060.11493726576681938No Hit
GGATCCGGCCCGGACTGGCT198790.11478136773729543No Hit
CGAAAATCTTTGCTCTGAAGTGGAA198680.11471785372526713No Hit
GTTGGATCCGGCCCGGACT195300.1127662413556708No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA193900.11195788120258354No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA191170.11038157890406342No Hit
CTCCCTTTCCGAGGTCGCGGTA187590.10831448651259747No Hit
GACCAAGATAGTCGAACTCGAC184660.10662270419220772No Hit
TGGATCCGGCCCGGACTGGCC182460.10535242395164204No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA180200.1040474997045155No Hit
TTCCTGCACGGCTTCAGGCAATTCTGGC179900.10387427967171108No Hit
GCACTGGTGGTTCAGTGGTAGAATTCA175030.10106234113918618No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)