FastQCFastQC Report
Mon 22 May 2023
sRNA-POC-47-S1-TP2_R2_001.fastp-trim.20230522.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-47-S1-TP2_R2_001.fastp-trim.20230522.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15409556
Sequences flagged as poor quality0
Sequence length15-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCA212691413.802565109598225No Hit
ATAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG6251274.056748942020134No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGC2907431.8867707804170346No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT1428640.9271130199987592Illumina RNA PCR Primer (100% over 50bp)
TTCCACTTCAGAGCAAAGATCTTCA1341850.8707908261600789No Hit
GAGAATTCTACCACTGAACCACCAGTGC1168180.7580880331659134No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGC1029910.668357998114936No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA1015890.6592597476526904Illumina RNA PCR Primer (100% over 50bp)
ACAAGTTCGGATCTACGGGT952490.6181164467035909No Hit
AAACTAACCAGCCTAGACTTGA909500.590218173709872No Hit
GGCGAGAATTCTACCACTGAACCACAGTGC861350.5589713292193493No Hit
TGACAGGGTCGAGTTCGACTATCTTGGTCG856310.5557006314782853No Hit
AACCGCTTCAAGTCTCAGCCCGAG785990.5100666106148678No Hit
GACCGCGACCTCGGAAAGGG701750.4553992340856544No Hit
GACCGCGACCTCGGAAAG693730.4501946714103897No Hit
GACCGCGACCTCGGAAAGGGAG564210.3661429310487596No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGC551350.3577974602253303No Hit
GTAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG542890.35230736044568706No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA535030.34720662944474195Illumina RNA PCR Primer (100% over 50bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAA521600.33849125828154947Illumina RNA PCR Primer (100% over 50bp)
CCACAAGTTCGGATCTACGGGT492030.319301866971378No Hit
ATCCACTTCAGAGCAAAGATTTTCA481800.3126631292945754No Hit
AGAATTCTACCACTGAACCACCAGTGC481070.3121893972804927No Hit
GCCAGAATTGCCTGAAGCCGTGCAGGAA473040.30697834512558314No Hit
CGCTTCAAGTCTCAGCCCGAG442200.2869647899005007No Hit
TCCACTTCAGAGCAAAGATTTTCA433970.28162394815269176No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGC431940.28030658378476314No Hit
GAGAATTCTACCACTGAACCACAGTGC370770.24061043679649172No Hit
CCAGAATTGCCTGAAGCCGTGCAGGAA356300.2312201597502225No Hit
TGCTTCAAGTCTCAGCCCGAG353720.22954587400182072No Hit
GCCAGTCCGGGCCGGATCC327730.2126797164045479No Hit
TGCGAGAATTCTACCACTGAACCACAGTGC322890.20953880825638327No Hit
GACGTACTGTCGTCAGCGTTCAAATATGGAAA322000.20896124456798107No Hit
CTCCACTTCAGAGCAAAGATTTTCA302080.1960342011152041No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAT287330.18646221863887577Illumina RNA PCR Primer (100% over 50bp)
GCAAGTTCGGATCTACGGGT263380.17091991488917657No Hit
TTCCACCTCAGAGCAAAGATTTTCA247340.16051078953864734No Hit
TTTTTTTGGTCTTTCACCTACAGCATTAATA232760.15104912821628347No Hit
AACCGCTTCAAGTCTCAGCCCGAGA232740.15103614925699352No Hit
TCACAAGTTCGGATCTACGGGT225770.14651298194445056No Hit
TGGCGAGAATTCTACCACTGAACCACCAGTGC223900.14529944925084148No Hit
CCACTTCAGAGCAAAGATTTTCA220120.14284642594504346No Hit
GGCCAGTCCGGGCCGGATCC215290.13971200727652375No Hit
TCAGAATTGCCTGAAGCCGTGCAGGAA214750.13936157537569543No Hit
GACCGCTTCAAGTCTCAGCCCGAG206370.13392339143321197No Hit
GTACTGTCGTCAGCGTTCAAATATGGAAA201770.13093823079652653No Hit
GAACTAACCAGCCTAGACTTGA201670.13087333600007683No Hit
TCCCACTTCAGAGCAAAGATTTTCA199060.12917958181274009No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAC197440.12812828611025523Illumina RNA PCR Primer (100% over 50bp)
TGCGTGAGCCTAGAGATCCACCGTCAAAAGTT188700.12245648090055289No Hit
GTCGTCAGCGTTCAAATATGGAAACAGA187930.12195679096789032No Hit
AAATCCATAGAATCTCAGCAGTT185880.12062644764067179No Hit
TGTACTGTCGTCAGCGTTCAAATATGGAAA184010.11941291494706271No Hit
CAGTTAAGAGCACACTC182790.11862119843037658No Hit
GACCCGAGGGTCACAGCTG178150.11561007987511125No Hit
GACGTACTGTCGTCAGCGTTCAATATGGAAA173040.11229395577653242No Hit
AGCGAGAATTCTACCACTGAACCACAGTGC167880.10894538427972877No Hit
ACTCGTTCTCAAAGTAGTTCAGCAGAA166330.10793951493475867No Hit
CAGTTAAGAGCACACT163150.10587586040765873No Hit
AGATCCACCGTCAAAAGTT159520.10352017929653522No Hit
TTCCCGGTCCGACCGCGACCTCGGAAAG158310.10273495225949406No Hit
GACCGCGACCTCGGAAA154300.10013267092186173No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)