FastQCFastQC Report
Mon 22 May 2023
sRNA-POC-47-S1-TP2_R1_001.fastp-trim.20230522.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-47-S1-TP2_R1_001.fastp-trim.20230522.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15409556
Sequences flagged as poor quality0
Sequence length15-150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA213067913.826998000461533No Hit
AACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGCC5943653.857119569181617No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC2952511.916025354656552No Hit
TGAAGATCTTTGCTCTGAAGTGGAA1342190.8710114684680078No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC1162810.7546031825965654No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA1025210.6653079426818008No Hit
ACCCGTAGATCCGAACTTGT953010.6184538996451293No Hit
TCAAGTCTAGGCTGGTTAGTTT900570.5844230683869152No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCA872240.5660383725527198No Hit
CAACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGC870320.564792392460886No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC864000.5606910413252659No Hit
CTCGGGCTGAGACTTGAAGCGGTT776690.5040313945450472No Hit
CCCTTTCCGAGGTCGCGGTC702780.45606765048908615No Hit
CTTTCCGAGGTCGCGGTC695520.4513562882668391No Hit
CTCCCTTTCCGAGGTCGCGGTC567470.3682585014130193No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT550160.3570252121475791No Hit
ACCCGTAGATCCGAACTTGTGG500050.3245064296466426No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT477250.3097104160561148No Hit
TTCCTGCACGGCTTCAGGCAATTCTGGC475420.3085228412810856No Hit
TGAAAATCTTTGCTCTGAAGTGGAT460790.2990287325604969No Hit
TGAAAATCTTTGCTCTGAAGTGGA437280.2837719659151762No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCT434320.28185107994026565No Hit
CTCGGGCTGAGACTTGAAGCG433320.28120213197576877No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC368640.23922817763211346No Hit
CTCGGGCTGAGACTTGAAGCA360190.23374456733211518No Hit
TTCCTGCACGGCTTCAGGCAATTCTGG350860.22768988282335975No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA328990.21349739083981392No Hit
GGATCCGGCCCGGACTGGC328210.2129912114275064No Hit
TTTCCATATTTGAACGCTGACGACAGTACGTC327600.21259535316916334No Hit
TGAAAATCTTTGCTCTGAAGTGGAG308270.2000511890154395No Hit
ACCCGTAGATCCGAACTTGC271330.1760790512069264No Hit
TGAAAATCTTTGCTCTGAGGTGGAA248870.16150367992432746No Hit
ACCCGTAGATCCGAACTTGTGA238000.1544496155502469No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA233760.1516980761807803No Hit
TCTCGGGCTGAGACTTGAAGCGGTT229320.14881674721841434No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCA223630.14512423330042734No Hit
TTCCTGCACGGCTTCAGGCAATTCTGA217980.1414576773010202No Hit
TGAAAATCTTTGCTCTGAAGTGG215900.1401078655348668No Hit
GGATCCGGCCCGGACTGGCC212770.1380766584059917No Hit
CTCGGGCTGAGACTTGAAGCGGTC211610.13732387876717536No Hit
TCAAGTCTAGGCTGGTTAGTTC206810.13420892853759056No Hit
TTTCCATATTTGAACGCTGACGACAGTAC204940.13299539584398148No Hit
TGAAAATCTTTGCTCTGAAGTGGGA201620.130840888601852No Hit
TCTGTTTCCATATTTGAACGCTGACGAC189830.12318979210043429No Hit
AACTTTTGACGGTGGATCTCTAGGCTCACGCA189510.12298212875179532No Hit
TTTCCATATTTGAACGCTGACGACAGTACA185320.12026303678055357No Hit
AACTGCTGAGATTCTATGGATTT183990.11939993598777277No Hit
CAGCTGTGACCCTCGGGTC181370.11769969232079108No Hit
GAGTGTGCTCTTAACTG177990.11550624820079176No Hit
TTTCCATATTGAACGCTGACGACAGTACGTC176280.11439654718150218No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT166820.10825749943736213No Hit
TTCTGCTGAACTACTTTGAGAACGAGT165730.10755014615606057No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA159400.1034423055407956No Hit
AGTGTGCTCTTAACTG158970.10316325791606196No Hit
AACTTTTGACGGTGGATCT156580.10161227228091452No Hit
TTTCCGAGGTCGCGGTC155420.10085949264209819No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)