FastQCFastQC Report
Mon 22 May 2023
sRNA-POC-57-S1-TP2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-57-S1-TP2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26459749
Sequences flagged as poor quality0
Sequence length150
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGA18836477.118914846849076TruSeq Adapter, Index 7 (97% over 37bp)
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGAT17411386.580326971355624TruSeq Adapter, Index 7 (97% over 37bp)
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA9274893.5052826842764078Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC6877472.5992196675788573Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGGGCTGAGACTTGAAGCAAGATCGGAAGAGCACACGTCTGAACTCCA4603471.7398010842808824Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGCC2807561.0610682663694202No Hit
GTTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTC2375160.8976502384810983Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT2026660.7659407502316065No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA1779260.6724402412131726Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG1647520.6226514091271236Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGA1590920.6012604276782821TruSeq Adapter, Index 7 (97% over 37bp)
TTCCGAGGTCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1581160.5975718061422276Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGACTGCTGCCGGTCGTTGGGGCGAGATCGGAAGAGCACACGTCTGAACT1522720.5754854288300316Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGGACCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGA1463550.5531231607677004TruSeq Adapter, Index 7 (97% over 37bp)
CGGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGAT1439400.5439960900611718TruSeq Adapter, Index 7 (97% over 37bp)
CAGCTGTGACCCTCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC1434330.5420799721116024Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTCCGAGGTCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAA1336800.5052202120284663TruSeq Adapter, Index 2 (97% over 36bp)
CTCGGGCTGAGACTTGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAG1320810.4991770708029014Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGACCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGAT1245760.47081323409379283TruSeq Adapter, Index 7 (97% over 37bp)
CTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGA1238820.4681903823048359Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAACT1129160.4267463005790418Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1071610.40499628322249015Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1001250.3784049500998668Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTTGGATCCGGCCCGGACTGGCAAGATCGGAAGAGCACACGTCTGAACTC932680.35249011621387644Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGCCGTC905230.34211586814372275RNA PCR Primer, Index 28 (100% over 34bp)
CGACTGCTGCCGGTCGTTGGGGCAAGATCGGAAGAGCACACGTCTGAACT889450.3361520927503885Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTGGATCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA793830.3000141838080172TruSeq Adapter, Index 7 (100% over 35bp)
CGACCAAGATAGTCGAACTCGACCCTGTCAAGATCGGAAGAGCACACGTC755090.28537307742412826No Hit
GGGGGACTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAA737990.27891043108534397TruSeq Adapter, Index 2 (97% over 36bp)
CTCGGGCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGA687190.2597114583362072TruSeq Adapter, Index 7 (97% over 37bp)
AGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC678460.25641210731061737Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGTGTAGAATAAGTGGGAGCAGCTAGATCGGAAGAGCACACGTCTGAA678380.2563818727078628Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAG652190.24648381963109323TruSeq Adapter, Index 7 (97% over 37bp)
CGTGGTGGACTGCTTGCAGTGCTAGATCGGAAGAGCACACGTCTGAACTC640280.2419826431460102Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCCGAGGTCGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAA628370.23748146666092715TruSeq Adapter, Index 2 (97% over 36bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC622600.23530079593725547No Hit
CTCGGGCTGAGACTTGAAGCGGAGATCGGAAGAGCACACGTCTGAACTCC609580.2303801143389531Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC606260.22912537832463944Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT598690.22626442903899052No Hit
GTTGGATCCGGCCCGGACTGGCTAGATCGGAAGAGCACACGTCTGAACTC574360.21706933047626414Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CACTTTCTGGAGTTAAGGCTAGATCGGAAGAGCACACGTCTGAACTCCAG557000.21050842167852765Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCACTGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGAA545800.20627557729289117Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCACTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA521970.19726944499738075Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAAGGAATCGGCCGTGGATTGGCCAGATCGGAAGAGCACACGTCTGAAC496480.18763594469471345Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCGCGTATGCCGTC478020.18065931010910194RNA PCR Primer, Index 28 (97% over 34bp)
CGACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTCT476570.1801113079341758Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCA470160.17768876038846776Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATG469120.17729571055265866TruSeq Adapter, Index 7 (97% over 37bp)
TTCCGAGGTCGCGGTCGGACCGAGATCGGAAGAGCACACGTCTGAACTCC458420.1732518324342381Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTTCCGAGGTCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC454290.1716909710670347Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGACTGGCCGTGGTCGAGGTAGATCGGAAGAGCACACGTCTGAACTCCA450800.1703719865218676Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCGGGCTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAG442240.16713688402713117TruSeq Adapter, Index 7 (97% over 37bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTG437460.16533036651254704Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGGAGTCTCGGCTCCGGACTGTTACAGCCCGGCTAGATCGGAAGAGCAC428460.16192897370266057No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT387870.14658869213007272No Hit
CAACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGC387320.14638082923613524No Hit
CCGAGCTGTTCTTCCTCGCCGAGACCAGATCGGAAGAGCACACGTCTGAA381620.1442266137898738Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGTGTGCTCTTAACTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA380260.14371262554304653Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTTGGATCCGGCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA372260.14068916526759193TruSeq Adapter, Index 7 (100% over 35bp)
GTTGGATCCGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAA371890.14054933022985214TruSeq Adapter, Index 2 (97% over 36bp)
CGACTGCTGCCGGTCGTTGGGGCAGATCGGAAGAGCACACGTCTGAACTC370700.14009959051387827Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT350200.1323519735580258Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCAAGTCTAGGCTGGTTAGTTTAGATCGGAAGAGCACACGTCTGAACTCC347680.1313995835712576Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAG341890.12921135419689733TruSeq Adapter, Index 7 (97% over 37bp)
CAGCTGTGACCCTCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT339050.12813802579911096Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAAGATCGGAAGAGCACACGTCT335230.12669432351758136Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGCTGTGACCCTCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC331750.1253791182977586Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCACTGGTGGTTCAGTGGTAGAATTCCAGATCGGAAGAGCACACGTCTGA330500.12490670262971881Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTGAACTCCAGT319820.12087038316198691Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGGGAAGGAATCGGCCGTGGATTGGCCAGATCGGAAGAGCACACGTCTG317580.12002381428485961Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCGAGGTCGCGGTCGGACCAAGATCGGAAGAGCACACGTCTGAACTCC309470.1169587814306175Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTGGATCCGGCCCGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGT309340.11690965020114137Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCACTTTCTGGAGTTAAGGCTAGATCGGAAGAGCACACGTCTGAACTCCA306840.1159648188650618Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTTTCCGAGGTCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC299510.1131945733876765Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTTGGATCCGGCCCGGACTGGCAGATCGGAAGAGCACACGTCTGAACTCC293940.11108948917089123Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCC293790.11103279929072646Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTGGATCCGGCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA266550.1007379170528035TruSeq Adapter, Index 7 (100% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)