FastQCFastQC Report
Mon 22 May 2023
sRNA-POC-53-S1-TP2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-53-S1-TP2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25726329
Sequences flagged as poor quality0
Sequence length150
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTAT18902657.347589312101233TruSeq Adapter, Index 27 (97% over 41bp)
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA15828786.152755023851246Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTA14997825.829755189712453TruSeq Adapter, Index 27 (97% over 39bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC9187673.5713101546668393Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAAAATCTTTGCTCTGAGGTGGAAAGATCGGAAGAGCACACGTCTGAAC5397072.097878014387517Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGGGCTGAGACTTGAAGCAAGATCGGAAGAGCACACGTCTGAACTCCA4796541.86444789693858Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGCC4012621.55973283246125No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1653340.6426645636071902No Hit
CGGACCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTAT1558530.6058112682924952TruSeq Adapter, Index 27 (97% over 41bp)
CCGGACCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTA1359050.5282720282400183TruSeq Adapter, Index 27 (97% over 39bp)
CGGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTAT1338520.5202918768550305TruSeq Adapter, Index 27 (97% over 41bp)
GTTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTC1310490.5093964241847331Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTA1080190.41987723938382343TruSeq Adapter, Index 27 (97% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATTCCTATCTCGTATGCCGTC1064990.41396889544559584TruSeq Adapter, Index 27 (98% over 50bp)
CTCGGGCTGAGACTTGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAG1003240.3899662481965461Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAACT993720.3862657590983929Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCA982140.38176453391387477Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCC980120.3809793461010314Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA967990.37626433215559046Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGACTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATT904120.3514376264098932TruSeq Adapter, Index 8 (97% over 36bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG867550.3372226173427231Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTTCCGAGGTCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA860700.3345599755021402Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTATCTCGTATG844180.3281385385376981TruSeq Adapter, Index 27 (97% over 49bp)
CTCGGGCTGAGACTTGAAGCGGAGATCGGAAGAGCACACGTCTGAACTCC732140.2845878244035517Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATTCCTATCGCGTATGCCGTC731740.2844323416683352TruSeq Adapter, Index 27 (97% over 38bp)
CGACTGCTGCCGGTCGTTGGGGCGAGATCGGAAGAGCACACGTCTGAACT723830.28135767057942856Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAAAATCTTTGCTCTGAAGTGGAGAGATCGGAAGAGCACACGTCTGAAC720790.28017600179178304Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT629860.24483088900868832Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGGATCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT604960.23515208874146015TruSeq Adapter, Index 1 (100% over 35bp)
CAACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGC572010.22234419842799957No Hit
TCAAGTCTAGGCTGGTTAGTTTAGATCGGAAGAGCACACGTCTGAACTCC553220.21504039694120372Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGGGCTGAGACTTGAAACGAGATCGGAAGAGCACACGTCTGAACTCCA541770.2105897036456309Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTATCGCGTATG535910.20831188157470892TruSeq Adapter, Index 27 (97% over 42bp)
ACCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC527900.2051983398019982Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTGGATCCGGCCCGGACTGGCAAGATCGGAAGAGCACACGTCTGAACTC521870.20285443756860919Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACCAAGATAGTCGAACTCGACCCTGTCAAGATCGGAAGAGCACACGTC513450.1995815259923015No Hit
TCCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCT498230.19366540791731304TruSeq Adapter, Index 27 (100% over 39bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC494990.19240599776205924No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT490180.19053631787108063No Hit
TCTTGCATCACTTTTAAATAATTCTGTGGAAGATCGGAAGAGCACACGTC489590.19030698083663627No Hit
TGAAAATCTTTGCTCTGAGGTGGAGAGATCGGAAGAGCACACGTCTGAAC488270.18979388781042175Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCT442320.17193280860242438TruSeq Adapter, Index 27 (100% over 39bp)
ACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG425680.1654647268174173Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGA420990.16364169174700363Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCC411150.1598168164606773TruSeq Adapter, Index 27 (100% over 38bp)
CTTTCCGAGGTCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC387970.15080659195488016Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGAAGGAATCGGCCGTGGATTGGCCAGATCGGAAGAGCACACGTCTGAAC377040.14655802621508882Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGAGCTGTTCTTCCTCGCCGAGACCGAGATCGGAAGAGCACACGTCTGA367340.1427875698860883Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGCTGTGACCCTCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC337110.13103696217210004Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTTGGATCCGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATT317490.12341053400972987TruSeq Adapter, Index 8 (97% over 36bp)
CGACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTCT306810.11925914497944888Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCGGGCTGAGACTTGAAGCAAGATCGGAAGAGCACACGTCTGAACTCC300350.11674809880570212Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCCTGCACGGCTTCAGGCAATTCTGGCAGATCGGAAGAGCACACGTCTG299690.11649155229259488Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC296360.11519715852191738Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAAGATCGGAAGAGCACACGTCT289510.11253451668133453Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACTGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGAA286670.11143058926129726Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGGATCCGGCCCGGACTGGCTAGATCGGAAGAGCACACGTCTGAACTC280550.10905170341248453Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTTCCGAGGTCGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA278930.10842199833485763Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT275710.10717036231636468No Hit
GTTGGATCCGGCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT273580.10634241675133674TruSeq Adapter, Index 1 (100% over 35bp)
CACTACCGATAGCCAACTCGAACGTTTCCGAGATCGGAAGAGCACACGTC269620.10480313767269323No Hit
CGACTGCTGCCGGTCGTTGGGGCAAGATCGGAAGAGCACACGTCTGAACT267830.1041073524325993Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGAGCTGTTCTTCCTCGCCGAGACCAGATCGGAAGAGCACACGTCTGAA267350.10392077315033948Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGTGTGCTCTTAACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA266020.10340379305574456Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCACTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA263080.10226099495190316Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGGATCCGGCCCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA258860.10062065209536893Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)