FastQCFastQC Report
Mon 22 May 2023
sRNA-POC-50-S1-TP2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-50-S1-TP2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26073937
Sequences flagged as poor quality0
Sequence length150
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG595722522.8474319010589Illumina RNA PCR Primer (100% over 37bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA293989911.275240098953985Illumina DpnII expression Sequencing Primer (100% over 25bp)
GAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG7348572.8183584243530238Illumina RNA PCR Primer (100% over 36bp)
ATAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG6431922.466800468222348No Hit
TAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG5781662.2174096685130444Illumina RNA PCR Primer (100% over 37bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT5178281.9859985087790923Illumina RNA PCR Primer (100% over 50bp)
AAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG4840381.856405497949926Illumina RNA PCR Primer (100% over 37bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC3182101.2204140862962123No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA2548300.9773361038649437Illumina RNA PCR Primer (100% over 45bp)
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1178380.4519378872473305No Hit
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT1169310.44845931782377163Illumina DpnII expression Sequencing Primer (100% over 22bp)
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG1117700.4286656058116578Illumina DpnII expression Sequencing Primer (96% over 29bp)
AAACTAACCAGCCTAGACTTGAGATCGTCGGACTGTAGAACTCTGAACGT932810.3577557159856603Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCTAGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC892750.3423917147609891Illumina RNA PCR Primer (100% over 32bp)
TGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG819160.3141681288867117Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT809380.3104172568952667No Hit
GTGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG791290.3034792942853241Illumina RNA PCR Primer (100% over 37bp)
TAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG738820.2833557509937989Illumina RNA PCR Primer (100% over 36bp)
ACAAGTTCGGATCTACGGGTGATCGTCGGACTGTAGAACTCTGAACGTGT698940.268060784222958Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGGTCCGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT670350.2570958118062493Illumina RNA PCR Primer (100% over 35bp)
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC614960.23585237626369965Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC603310.23138431300190684No Hit
ATCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA599180.22980035581124553Illumina DpnII expression Sequencing Primer (100% over 25bp)
AAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG590880.22661710044018288Illumina RNA PCR Primer (100% over 36bp)
GTAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG568070.217868901040913No Hit
CCACAAGTTCGGATCTACGGGTGATCGTCGGACTGTAGAACTCTGAACGT528250.20259694575468215Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG497680.19087259434584045Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG466010.17872636571914707No Hit
GCCAGAATTGCCTGAAGCCGTGCAGGAAGATCGTCGGACTGTAGAACTCT458180.1757233669775301Illumina DpnII expression Sequencing Primer (100% over 22bp)
CAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG430580.1651380840568879Illumina RNA PCR Primer (100% over 37bp)
CTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA418080.16034402476311882Illumina DpnII expression Sequencing Primer (100% over 25bp)
AACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC417190.16000268774140244Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG396460.15205221980861577Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG385720.14793316406340937Illumina RNA PCR Primer (100% over 37bp)
GAGGTCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG362860.13916578842696445Illumina RNA PCR Primer (100% over 37bp)
GGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG355220.13623565938661275Illumina DpnII expression Sequencing Primer (96% over 26bp)
TCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTG351560.13483195882539717Illumina DpnII expression Sequencing Primer (100% over 23bp)
CCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAACG348710.13373891330641782Illumina DpnII expression Sequencing Primer (96% over 26bp)
TGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT324910.12461102441108146No Hit
GAGGTCCGGATCGTCGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT323730.12415846521374965Illumina RNA PCR Primer (97% over 36bp)
GAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTG320150.12278544663201418Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA315520.12100972706960211No Hit
TTTTTTTGGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAAC290390.11137175026540871No Hit
CCAGAATTGCCTGAAGCCGTGCAGGAAGATCGTCGGACTGTAGAACTCTG279800.10731022323172752Illumina DpnII expression Sequencing Primer (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)