FastQCFastQC Report
Mon 22 May 2023
sRNA-POC-50-S1-TP2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-50-S1-TP2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26073937
Sequences flagged as poor quality0
Sequence length150
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCAT608061623.32066691731287TruSeq Adapter, Index 1 (97% over 37bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC301630211.568264508731458Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCA7520102.8841444236058407TruSeq Adapter, Index 1 (97% over 37bp)
AACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGCC6086172.3341967881566945No Hit
CGGACCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCAT5919862.2704127880649554TruSeq Adapter, Index 1 (97% over 37bp)
CGGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCAT4822021.8493639836592377TruSeq Adapter, Index 1 (97% over 37bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT3316901.272113221720218No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATGCCGTC1862180.7141921068536753RNA PCR Primer, Index 26 (100% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATG1371420.5259735037328654TruSeq Adapter, Index 1 (97% over 37bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCGCGTATGCCGTC1301800.4992725110902891RNA PCR Primer, Index 26 (97% over 34bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT1236820.4743510732575599No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG1200470.46040994883127934Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA1132250.4342458908296051Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCAAGTCTAGGCTGGTTAGTTTAGATCGGAAGAGCACACGTCTGAACTCC945700.362699349929395Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGC922330.3537363766737643No Hit
GGGGGACTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATG914300.35065667298344705TruSeq Adapter, Index 15 (100% over 36bp)
CTCGGGCTGAGACTTGAAGCAAGATCGGAAGAGCACACGTCTGAACTCCA853940.32750711946569483Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGACCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCAT844720.3239710213306107TruSeq Adapter, Index 1 (97% over 37bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC840840.3224829453258248No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCGCGTATG829840.318264173147308TruSeq Adapter, Index 1 (97% over 37bp)
CCGGACCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCA758150.29076928428568344TruSeq Adapter, Index 1 (97% over 37bp)
ACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG720230.2762260260121055Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGC681290.261291572500156TruSeq Adapter, Index 1 (97% over 37bp)
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT630130.24167044662261783Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT621750.23845650927207504No Hit
TGAAAATCTTTGCTCTGAAGTGGATAGATCGGAAGAGCACACGTCTGAAC597100.22900262434476237Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCA590190.22635246836716683TruSeq Adapter, Index 1 (97% over 37bp)
ACCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC555700.21312469996379912Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCA511190.19605401363054611Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCCTGCACGGCTTCAGGCAATTCTGGCAGATCGGAAGAGCACACGTCTG473060.18143021516083283Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAAAATCTTTGCTCTGAAGTGGAGAGATCGGAAGAGCACACGTCTGAAC436360.16735485707432676Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAACT428110.16419077794043915Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCAT403860.15489030291052708TruSeq Adapter, Index 1 (97% over 37bp)
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA402370.15431885104270981Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCAT368980.14151295985719378TruSeq Adapter, Index 1 (97% over 37bp)
CTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGA364250.13969888782043158Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTC362990.13921564664361963Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAAAATCTTTGCTCTGAAGTGGAGATCGGAAGAGCACACGTCTGAACTC350650.1344829513088108Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC341090.13081645476093617No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA327850.12573858715697595Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG324380.12440775629702565No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAAAGATCGGAAGAGCACACGT301150.11549847650548514No Hit
TTCCTGCACGGCTTCAGGCAATTCTGGAGATCGGAAGAGCACACGTCTGA283530.10874077052498822Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGAAAATCTTTGCTCTGAAGTGGGAAGATCGGAAGAGCACACGTCTGAAC268800.10309145105321073Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)