FastQCFastQC Report
Mon 22 May 2023
sRNA-POC-48-S1-TP2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-48-S1-TP2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24884997
Sequences flagged as poor quality0
Sequence length150
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATAT395054815.875219916642946TruSeq Adapter, Index 25 (100% over 41bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC311767612.528335848302493Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG9776453.928652271888962Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATA6055522.433401940936541TruSeq Adapter, Index 25 (100% over 40bp)
AACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGCC5300342.129933951770217No Hit
CGGACCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATAT3620821.4550212724558496TruSeq Adapter, Index 25 (100% over 41bp)
CGGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATAT2986851.200261346223992TruSeq Adapter, Index 25 (100% over 41bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACTGATATCTCGTATGCCGTC2401650.9650995738516666TruSeq Adapter, Index 25 (100% over 50bp)
GCACTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA2332620.937359968337549Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1837210.738280177409706No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA1632940.6561945737827495Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACTGATATCGCGTATGCCGTC1610700.6472574619960774TruSeq Adapter, Index 25 (98% over 50bp)
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA1368520.5499377797795193Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCTTTCCGAGGTCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAG1160490.46634122559870106Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATCTCGTATG1151550.4627486995477636TruSeq Adapter, Index 25 (100% over 49bp)
TCCCTTTCCGAGGTCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCA1145130.4601688318467549Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTTTCCGAGGTCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1110640.44630907530348507Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCGGGCTGAGACTTGAAGCAAGATCGGAAGAGCACACGTCTGAACTCCA949480.38154716273423706Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCCCTTTCCGAGGTCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCC937320.3766606843472796Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAG842780.338669922282892Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAACT803660.32294960694590397Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTC759930.305376769786229Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACCAAGATAGTCGAACTCGACCCTGTCAAGATCGGAAGAGCACACGTC746540.29999601768085404No Hit
GGGGGACTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT729810.293273091413272TruSeq Adapter, Index 8 (100% over 36bp)
CAACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGC722390.29029137516070425No Hit
TCAAGTCTAGGCTGGTTAGTTTAGATCGGAAGAGCACACGTCTGAACTCC689510.27707859478544444Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT681130.27371110392338Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT669570.269065734667358No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATCGCGTATG634310.25489655473938777TruSeq Adapter, Index 25 (97% over 49bp)
CCGGACCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATA579810.23299580867942238TruSeq Adapter, Index 25 (100% over 40bp)
TGAAAATCTTTGCTCTGAAGTGGATAGATCGGAAGAGCACACGTCTGAAC563690.22651801002829133Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG555890.22338359132613117Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCTTTCCGAGGTCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT530330.21311234234828316Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGATCCGGCCCGGACTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT507440.2039140290030977Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGACCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATAT497860.20006431987916254TruSeq Adapter, Index 25 (100% over 41bp)
TCTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAAC489910.1968696238942685Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCC477560.1919067942825149Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGAT470770.1891782426174293TruSeq Adapter, Index 25 (100% over 39bp)
CCGGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATA454110.1824834457484564TruSeq Adapter, Index 25 (100% over 40bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC448530.18024113083075718No Hit
ACCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC425470.17097450323180668Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGATCCGGCCCGGACTGGCAAGATCGGAAGAGCACACGTCTGAACTCCAG421890.1695358854172255Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTTGGATCCGGCCCGGACTGGCAAGATCGGAAGAGCACACGTCTGAACTC397910.15989955715084073Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCTGCTGAACTACTTTGAGAACGAGTAGATCGGAAGAGCACACGTCTGA370730.14897731351946716Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGAA364950.14665462889145617Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGAAAATCTTTGCTCTGAAGTGGAGAGATCGGAAGAGCACACGTCTGAAC363090.14590719058555643Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA332720.13370305007470967Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT330450.13279085386267075No Hit
TCTCGGGCTGAGACTTGAAGCAAGATCGGAAGAGCACACGTCTGAACTCC329850.13254974473173534Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAAAATCTTTGCTCTGAAGTGGAGATCGGAAGAGCACACGTCTGAACTC325390.13075750019178223Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCACTTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG305170.12263212247925928Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCCGAGGTCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA304550.12238297637729272Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCACTGGTGGTTCAGTGGTAGAATTCCAGATCGGAAGAGCACACGTCTGA300210.12063895366352666Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC289150.11619450868328414Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATAT281720.1132087739452008TruSeq Adapter, Index 25 (100% over 41bp)
TGAAAATCTTTGCTCTGAAGTGGGAAGATCGGAAGAGCACACGTCTGAAC264780.10640145948179137Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAAAATCTTTGCTCTGAGGTGGAAAGATCGGAAGAGCACACGTCTGAAC255760.10277678554672923Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC253720.10195701450154886No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)