FastQCFastQC Report
Mon 22 May 2023
sRNA-POC-47-S1-TP2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-47-S1-TP2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27579504
Sequences flagged as poor quality0
Sequence length150
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG723693926.24027973817078Illumina RNA PCR Primer (100% over 37bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA20666087.493274715890467Illumina DpnII expression Sequencing Primer (100% over 25bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT6574172.3837158202700093Illumina RNA PCR Primer (100% over 50bp)
TAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG6323202.2927170843971667Illumina RNA PCR Primer (100% over 37bp)
GAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG6300982.2846603767783495Illumina RNA PCR Primer (100% over 36bp)
ATAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG6271902.274116314782166No Hit
AAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG5708222.069732653640181Illumina RNA PCR Primer (100% over 37bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA3575471.2964228798313415Illumina RNA PCR Primer (100% over 45bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC2716940.9851301169158081No Hit
TTCCACTTCAGAGCAAAGATCTTCAGATCGTCGGACTGTAGAACTCTGAA1307240.473989670010019Illumina DpnII expression Sequencing Primer (100% over 25bp)
GTGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG1183630.42917015476420467Illumina RNA PCR Primer (100% over 37bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT1128190.4090682704083438Illumina DpnII expression Sequencing Primer (100% over 22bp)
GAGGTCCGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT988210.3583131879384053Illumina RNA PCR Primer (100% over 35bp)
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC957190.34706570502500694No Hit
ACAAGTTCGGATCTACGGGTGATCGTCGGACTGTAGAACTCTGAACGTGT913850.3313511366991952Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGCTAGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC885490.32106813813620433Illumina RNA PCR Primer (100% over 32bp)
AAACTAACCAGCCTAGACTTGAGATCGTCGGACTGTAGAACTCTGAACGT880800.3193675999394333Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT833040.3020503922042978No Hit
TGACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACT831790.3015971570772266No Hit
AACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC756130.2741637413058625Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG691250.2506390252703602No Hit
GACCGCGACCTCGGAAAGGGGATCGTCGGACTGTAGAACTCTGAACGTGT675990.24510593083907528Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAG671940.24363744902736467Illumina DpnII expression Sequencing Primer (96% over 30bp)
TAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG573660.20800229039652057Illumina RNA PCR Primer (100% over 36bp)
GACCGCGACCTCGGAAAGGGAGGATCGTCGGACTGTAGAACTCTGAACGT546010.19797672938570615Illumina DpnII expression Sequencing Primer (96% over 28bp)
GTAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG545930.19794772233757357No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC514490.18654795242147937No Hit
AAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG485660.17609453745070977Illumina RNA PCR Primer (100% over 36bp)
CAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG473390.17164558144337913Illumina RNA PCR Primer (100% over 37bp)
CCACAAGTTCGGATCTACGGGTGATCGTCGGACTGTAGAACTCTGAACGT472410.17129024510375532Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGGTCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG468870.17000668322388973Illumina RNA PCR Primer (100% over 37bp)
ATCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA466050.1689841847772172Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG464450.16840404381456606Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCCAGAATTGCCTGAAGCCGTGCAGGAAGATCGTCGGACTGTAGAACTCT462250.1676063499909208Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG450160.16322265984188838Illumina RNA PCR Primer (100% over 37bp)
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG426500.15464382535668517Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGGTCCGGATCGTCGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT425180.154165209062498Illumina RNA PCR Primer (97% over 36bp)
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC421250.15274023782298624Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA405790.14713462577136993No Hit
GAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTG358830.13010748851756002Illumina DpnII expression Sequencing Primer (100% over 23bp)
CCAGAATTGCCTGAAGCCGTGCAGGAAGATCGTCGGACTGTAGAACTCTG347370.12595222887257146Illumina DpnII expression Sequencing Primer (100% over 23bp)
TGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG341790.12392898726532574Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCCAGTCCGGGCCGGATCCGATCGTCGGACTGTAGAACTCTGAACGTGTA314290.11395781446975986Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT314090.11388529684942847No Hit
GACGTACTGTCGTCAGCGTTCAAATATGGAAAGATCGTCGGACTGTAGAA305590.11080329798534448No Hit
GAGGTCCGGATCGTCGGACTGTAAACTCTGAACGTGTAGATCTCGGTGGT299230.10849723765880633Illumina RNA PCR Primer (100% over 27bp)
CTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA293460.1064051043122458Illumina DpnII expression Sequencing Primer (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)