FastQCFastQC Report
Mon 22 May 2023
sRNA-POC-47-S1-TP2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-47-S1-TP2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27579504
Sequences flagged as poor quality0
Sequence length150
%GC71

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCAT689568525.002933337742405TruSeq Adapter, Index 22 (97% over 37bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC20600267.469409167039408Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGACCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCAT6376342.311985016119217TruSeq Adapter, Index 22 (97% over 37bp)
CCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCA6041252.1904853691349926TruSeq Adapter, Index 22 (97% over 37bp)
AACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGCC5973252.1658293782223206No Hit
CGGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCAT5398761.9575261397014247TruSeq Adapter, Index 22 (97% over 37bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT2762061.0014900920625693No Hit
TGAAGATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC1297960.4706248524266426Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGACCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCAT1242700.45058823392908015TruSeq Adapter, Index 22 (97% over 37bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG1119090.4057687186832657Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT1009260.3659456674782839No Hit
TCCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGC946790.3432947887677748TruSeq Adapter, Index 22 (97% over 37bp)
ACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG899560.3261697527265175Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGC875070.31728997011693905No Hit
GGGGGACTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGT863970.31326524218854696TruSeq Adapter, Index 9 (97% over 36bp)
CGACCAAGATAGTCGAACTCGACCCTGTCAAGATCGGAAGAGCACACGTC861110.31222824021780815No Hit
TCAAGTCTAGGCTGGTTAGTTTAGATCGGAAGAGCACACGTCTGAACTCC855070.3100382080838002Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC844390.3061657671581041No Hit
CGGACCTCAGATCGGAAGAGCACACGTCTAAACTCCAGTCACGGTAGCAT801250.29052371645262365TruSeq Adapter, Index 11 (97% over 35bp)
CTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAACT746280.27059224850454167Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCTTTCCGAGGTCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAG666780.24176649442281486Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTTTCCGAGGTCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC659140.23899632132615584Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGACCTCAAATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCAT620610.22502580176931392TruSeq Adapter, Index 11 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTTTG602870.21859348884591978TruSeq Adapter, Index 22 (97% over 37bp)
CCGGACCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCA582260.21112054807077024TruSeq Adapter, Index 22 (97% over 37bp)
CTCCCTTTCCGAGGTCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCC542950.19686720979463587Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT517440.18761758732136732No Hit
ACCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC472100.17117784279224166Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGACCTCAGATCGGAGAGCACACGTCTGAACTCCAGTCACGGTAGCATC466780.16924887409142672TruSeq Adapter, Index 22 (96% over 30bp)
CGGACCTCAGATCGGAAAGCACACGTCTGAACTCCAGTCACGGTAGCATC465210.16867961077182533TruSeq Adapter, Index 11 (96% over 28bp)
CCGGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCA462030.16752658060855627TruSeq Adapter, Index 22 (97% over 37bp)
TTCCTGCACGGCTTCAGGCAATTCTGGCAGATCGGAAGAGCACACGTCTG458940.1664061833744363Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA456150.16539456257081345Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCAT449180.16286732350226457TruSeq Adapter, Index 22 (97% over 37bp)
CAGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCAT445910.16168166040984638TruSeq Adapter, Index 22 (97% over 37bp)
TGAAAATCTTTGCTCTGAAGTGGATAGATCGGAAGAGCACACGTCTGAAC444020.16099636889771476Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGGTTG432830.1569390080401736TruSeq Adapter, Index 22 (97% over 37bp)
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT417870.15151469003938578Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGACCTCAGATCGGAAAAGCACACGTCTGAACTCCAGTCACGGTAGCAT416170.15089829026656898TruSeq Adapter, Index 11 (97% over 35bp)
CGGACCTCAGATCGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATC407400.1477183926150376TruSeq Adapter, Index 11 (96% over 31bp)
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA406260.1473050421791487Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCAGTCACGGTAGCATC405550.14704760462697228Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG405220.14692795055342547No Hit
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAATCACGGTAGCAT399200.1447451701814507TruSeq Adapter, Index 11 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCGCGTTTG376380.1364709097016393TruSeq Adapter, Index 22 (97% over 37bp)
GCACTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA354260.12845046089298778Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATG353500.1281748939357285TruSeq Adapter, Index 22 (97% over 37bp)
CTCGGGCTGAGACTTGAAGCAAGATCGGAAGAGCACACGTCTGAACTCCA352230.12771440704662418Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGACCTCAGATCGGAAGAGCACACATCTGAACTCCAGTCACGGTAGCAT345920.1254264761251689TruSeq Adapter, Index 11 (97% over 35bp)
CGGACCTCAGATCGGAAGAACACACGTCTGAACTCCAGTCACGGTAGCAT343830.12466866699270589TruSeq Adapter, Index 11 (97% over 35bp)
CGGACCTCAGATCAGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCAT341240.12372956380941441TruSeq Adapter, Index 11 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCGCGGTTG337250.12228283728380322TruSeq Adapter, Index 22 (97% over 37bp)
TTCCTGCACGGCTTCAGGCAATTCTGGAGATCGGAAGAGCACACGTCTGA335590.12168094103505268Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC328310.11904129965499016No Hit
GGATCCGGCCCGGACTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT310410.11255097263533093Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTTCCATATTTGAACGCTGACGACAGTACGTCAGATCGGAAGAGCACACG309460.11220651393875683No Hit
CGGACCTCAGATCGGAAGACACACGTCTGAACTCCAGTCACGGTAGCATC309180.1121049892702929TruSeq Adapter, Index 11 (96% over 25bp)
TGAAAATCTTTGCTCTGAAGTGGAGAGATCGGAAGAGCACACGTCTGAAC302840.10980618070578789Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGACCTCAGATCGAAAGAGCACACGTCTGAACTCCAGTCACGGTAGCAT289350.10491486721443577TruSeq Adapter, Index 11 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)