FastQCFastQC Report
Mon 22 May 2023
sRNA-POR-82-S1-TP2_R2_001.fastp-trim.20230522.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POR-82-S1-TP2_R2_001.fastp-trim.20230522.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18224406
Sequences flagged as poor quality0
Sequence length15-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGAGAATTCTACCACTGAACCACCAGTGC5227292.868291015904716No Hit
TTCCACTTCAGAGCAAAGATTTTCA3872742.1250294796988167No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGC2658921.458988567309135No Hit
AGACAACTACTCACGCTTAAGCACAC2561571.4055711884381856No Hit
AGCAGACAACTACTCACGCTTAAGCACAC1854781.0177451051079525No Hit
AAGGATGATAACCCTCGACCGTCCGGACCTC1244820.6830510689895737No Hit
AGTAGATAACCCTCCCCAAGTCCGGACCTC1111070.6096604739819778No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAC1031540.566021191582321No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAA976170.5356388570359989No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGC958750.526080246456318No Hit
GGCGAGAATTCTACCACTGAACCACAGTGC865800.47507721239309525No Hit
AAACTAACCAGCCTAGACCTGA773600.4244857143766442No Hit
ACAAGTTCGGATCTACGGGA769120.4220274723905953No Hit
TCCGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG680650.37348268031342147No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT676400.3711506427150493Illumina RNA PCR Primer (100% over 50bp)
GTGGATAACCCTCCTCCGTCCGGACCTC668890.3670297951000433No Hit
AGAATCGTGGATAACCCTCCTCCGTCCGGACCTC554730.3043885216341208No Hit
AATGAGGTCAATGAAGGCGGGGAGGGGCGTACCGAAG549110.30130474485697917No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA535960.294089146170251Illumina RNA PCR Primer (100% over 50bp)
AAGCCTACAACACGGGGTATTCCCAGGCGGTCACC492780.27039564417078943No Hit
AGCGAGAATTCTACCACTGAACCACAGTGC440800.2418734525558748No Hit
ACGGGATCGGTCACGGTAA424200.23276478805399747No Hit
ACACATCCACTAGCGGTAGGTTGCCAG415610.22805132853164048No Hit
ACACTCTTCTTGGTGCAGGAGTCGACAGCT412640.22642164578642507No Hit
AATCGTGGATAACCCTCCTCCGTCCGGACCTC389800.21388900137540834No Hit
GGACAACTACTCACGCTTAAGCACAC386260.21194655123464656No Hit
AAATTCACTCAACCCA386140.2118807054671631No Hit
GCTCTTCTAGGTGCAGGAGTCGACAGCT382660.20997117821014302No Hit
AAACACAGCAGACAACTACTCACGCTTAAGCACAC378620.20775437070486685No Hit
TTCCACTTCAGAGAAAAGATTTTCA371690.20395177763269762No Hit
GCGAGAATTCTACCACTGAACCACCAGTGC369210.20259096510470628No Hit
AGCCGACAGTACCCTAAGCACTTCCCAACTAGTCCCC350850.19251656267973838No Hit
ATAACCCTCCTCCGTCCGGACCTC346610.19019001222865645No Hit
AAAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATAGAATC341720.18750679720370586No Hit
AGTAGTAGATAACCCTCCCCAAGTCCGGACCTC328530.18026924992781657No Hit
GGCAGACAACTACTCACGCTTAAGCACAC319950.17556127755274986No Hit
GAATCGTGGATAACCCTCCTCCGTCCGGACCTC297500.16324263188605434No Hit
AGGATGATAACCCTCGACCGTCCGGACCTC293110.1608337742256181No Hit
AGCAGAGATTGGTGAGCTGAGA288120.15809568772776464No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA278550.15284448777095946Illumina RNA PCR Primer (100% over 50bp)
AGTAGATAACCCTCCCCAAATCCGGACCTC271190.14880594736530783No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAA265060.14544232607636157Illumina RNA PCR Primer (100% over 50bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGC262870.1442406408197886No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGC247970.13606479135726016No Hit
ATGAGGTCAAGTTAGTGGAAGAGGGGTG247610.13586725405480982No Hit
GCTGAGTTCATCAACAGAATATTTAAACAA245060.13446803149578648No Hit
GATAACCCTCAACCGTCCGGACCTC237090.1300947751054273No Hit
GTCTCATGCTCTACCGACTGAGCTAGCCGGGC228170.12520023972249084No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGC222780.12224266733302583No Hit
AAGAATGGCGCAAGAATCCAAAGTGGTCA210850.1156965006157128No Hit
AATGAGGTCAAGTTAGTGGAAGAGGGGTG203110.11144944861303024No Hit
CGTGGATAACCCTCCTCCGTCCGGACCTC186410.10228591263825004No Hit
CGCTCTTCTAGGTGCAGGAGTCGACAGCT185920.10201704242102595No Hit
ACGAGAATTCTACCACTGAACCACCAGTGC185780.10194022235896193No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)