FastQCFastQC Report
Mon 22 May 2023
sRNA-POR-82-S1-TP2_R1_001.fastp-trim.20230522.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POR-82-S1-TP2_R1_001.fastp-trim.20230522.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18224406
Sequences flagged as poor quality0
Sequence length15-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC5277812.8960120840152483No Hit
TGAAAATCTTTGCTCTGAAGTGGAA3769142.068182633771438No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT2646361.4520967103125335No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCT2545221.3965997026185655No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCT1842851.0111989383906395No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTT1241050.6809824144611353No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACT1101490.6044037868778823No Hit
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGT1025860.5629044919214377No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGT971910.5333013322903364No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCT956440.5248127154322615No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC857600.4705777516150595No Hit
TCAGGTCTAGGCTGGTTAGTTT767520.4211495288241493No Hit
TCCCGTAGATCCGAACTTGT764480.4194814360479019No Hit
AACTTTTGACGGTGGATCTCTTGGCTCACGTATCGATGAAGAACGCAGCC685830.3763250226097904No Hit
GAGGTCCGGACGGAGGAGGGTTATCCAC663650.36415452991993263No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACGATTCT554210.3041031899750258No Hit
CTTCGGTACGCCCCTCCCCGCCTTCATTGACCTCATT545740.2994555762201523No Hit
GGTGACCGCCTGGGAATACCCCGTGTTGTAGGCTT489120.2683873482625442No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT439430.2411217133771054No Hit
TTACCGTGACCGATCCCGT421210.23112413101420148No Hit
CTGGCAACCTACCGCTAGTGGATGTGT414700.2275519981282243No Hit
AGCTGTCGACTCCTGCACCAAGAAGAGTGT412550.2263722614608125No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCC393170.21573817001223522No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACGATT389030.2134664910340562No Hit
TGGGTTGAGTGAATTT382330.20979010234956358No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGC382090.20965841081459663No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCTGTGTTT376680.2066898641305511No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC365610.2006155920802028No Hit
TGAAAATCTTTTCTCTGAAGTGGAA362190.19873898770692444No Hit
GGGGACTAGTTGGGAAGTGCTTAGGGTACTGTCGGCT349890.19198979653987078No Hit
GAGGTCCGGACGGAGGAGGGTTAT344830.18921330001098527No Hit
GATTCTATAGACACCCCTCCTCCAAATACTTGACCTCTTT342450.18790735895589683No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTACT327230.1795559207800792No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCC325130.17840361984911882No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACGATTC299690.1644443171426273No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCT291640.16002716357394584No Hit
TCTCAGCTCACCAATCTCTGCT286980.15747015293667183No Hit
GAGGTCCGGATTTGGGGAGGGTTATCTACT269620.14794446524073268No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACT260530.1429566483538613No Hit
CACCCCTCTTCCACTAACTTGACCTCAT249870.13710734934241478No Hit
GCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGT246790.13541730797700624No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGC241740.132646298595411No Hit
GAGGTCCGGACGGTTGAGGGTTATC233350.12804258201885976No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAC227830.12501367671462105No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA219160.12025632001394174No Hit
TGACCACTTTGGATTCTTGCGCCATTCTT210060.11526301597978009No Hit
CACCCCTCTTCCACTAACTTGACCTCATT205260.11262918528044205No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGT185070.10155063490135151No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)