FastQCFastQC Report
Mon 22 May 2023
sRNA-POR-79-S1-TP2_R2_001.fastp-trim.20230522.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POR-79-S1-TP2_R2_001.fastp-trim.20230522.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16757058
Sequences flagged as poor quality0
Sequence length15-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGAGAATTCTACCACTGAACCACCAGTGC5012462.9912529991839856No Hit
TTCCACTTCAGAGCAAAGATTTTCA4284642.5569166138829384No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGC2505861.4954056971098388No Hit
AGACAACTACTCACGCTTAAGCACAC2019281.205032530173256No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGC2009531.1992140863867633No Hit
AGCAGACAACTACTCACGCTTAAGCACAC1766511.0541886290540976No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAA1517570.9056303320069669No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAC1285830.7673363665626747No Hit
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGC1236250.737748833954027No Hit
GCTGAGTTCATCAACAGAATATTTAAACAA1124860.6712753515563412No Hit
AATGAGGTCAATGAAGGCGGGGAGGGGCGTACCGAAG671430.40068489349383407No Hit
AGTAGATAACCCTCCCCAAGTCCGGACCTC648420.386953366157711No Hit
AAGGATGATAACCCTCGACCGTCCGGACCTC621380.3708168820565042No Hit
AACAGAGTCGAGTTCGACTATCTTGAAC590820.35257979055750716No Hit
GGCGAGAATTCTACCACTGAACCACAGTGC588330.3510938495289567No Hit
ACACTCTTCTTGGTGCAGGAGTCGACAGCT555150.3312932377509226No Hit
GTCTCATGCTCTACCGACTGAGCTAGCCGGGC546980.3264176802395743No Hit
TCCGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG512160.30563837637847885No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGC508790.3036272835004808No Hit
GCTCTTCTAGGTGCAGGAGTCGACAGCT488400.29145927644339475No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT458210.2734429874265519Illumina RNA PCR Primer (100% over 50bp)
CGTGGATAACCCTCCTCCGTCCGGACCTC455590.2718794671475148No Hit
GTGGATAACCCTCCTCCGTCCGGACCTC441020.263184623458366No Hit
AAACTAACCAGCCTAGACCTGA431200.2573244062293035No Hit
AGCCTACTTCGTCAGGGGCTCAAGTCG426120.2542928478256744No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA400690.23911715290357055Illumina RNA PCR Primer (100% over 50bp)
ACAAGTTCGGATCTACGGGA384770.22961667853629197No Hit
AGCCGACAGTACCCTAAGCACTTCCCAACTAGTCCCC381800.22784429104440646No Hit
ACTGAGTTCATCAACAGAATATTTAAACAA376820.22487240898730554No Hit
ACAGAGTCGAGTTCGACTATCTTGAAC372460.22227052027867897No Hit
CGCTCTTCTAGGTGCAGGAGTCGACAGCT368640.21999088384130439No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCA359780.2147035595389119No Hit
AAGCCTACAACACGGGGTATTCCCAGGCGGTCACC349350.20847931659602778No Hit
GGGCGAGAATTCTACCACTGAACCACCAGTGC317560.18950820603473473No Hit
TTCCACTTCAGAGAAAAGATTTTCA309400.1846386161580392No Hit
AGTCTGATGCTCTACCGACTGAGCTAACCGGGC308320.18399411161553536No Hit
GCGAGAATTCTACCACTGAACCACCAGTGC307250.18335557470768438No Hit
AGCGAGAATTCTACCACTGAACCACAGTGC301800.1801032138219012No Hit
GGCAGACAACTACTCACGCTTAAGCACAC295450.17631376581736483No Hit
GGACAACTACTCACGCTTAAGCACAC287980.1718559427317134No Hit
AGAATTCTACCACTGAACCACCAGTGC277860.1658166964630665No Hit
AAGAGGTCTAGTATTTGGAGAAGGGGTG265380.15836908841635566No Hit
AGAATCGTGGATAACCCTCCTCCGTCCGGACCTC264260.15770071333524058No Hit
AGGCGAGAATTCTACCACTGAACCACAGTGC261970.15633412499974636No Hit
ATGAGGTCAAGTTAGTGGAAGAGGGGTG254360.15179275502895556No Hit
AGGATGATAACCCTCGACCGTCCGGACCTC234260.1397978093768011No Hit
AGTAGATAACCCTCCCCAAATCCGGACCTC231050.13788219865324808No Hit
AATCGTGGATAACCCTCCTCCGTCCGGACCTC230810.13773897542158056No Hit
AAAGAGGTCTAGTATTTGGAGAAGGGGTGTCTATAGAATC219390.13092393664806795No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGC215820.12879349107701363No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA213180.12721803552867098Illumina RNA PCR Primer (100% over 50bp)
AAATTCACTCAACCCA208360.12434163562601502No Hit
AATGAGGTCAAGTTAGTGGAAGAGGGGTG206450.12320181740732772No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAA198540.11848141839695249Illumina RNA PCR Primer (100% over 50bp)
GCAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAA187630.11197072899073333No Hit
AAACACAGCAGACAACTACTCACGCTTAAGCACAC187220.111726055969968No Hit
ACTCTTCTTGGTGCAGGAGTCGACAGCT185860.11091445765718541No Hit
AGTAGTAGATAACCCTCCCCAAGTCCGGACCTC176440.10529294581423541No Hit
TCTCATGCTCTACCGACTGAGCTAGCCGGGC171920.10259557495116385No Hit
AACCGGTGTTCTCCCTGTGGTATGGTCGTAGGC170780.10191526460074316No Hit
AAGCCTACAACACGGGGTATTCCCA170590.10180187954233971No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)