FastQCFastQC Report
Mon 22 May 2023
sRNA-POR-79-S1-TP2_R1_001.fastp-trim.20230522.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POR-79-S1-TP2_R1_001.fastp-trim.20230522.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16757058
Sequences flagged as poor quality0
Sequence length15-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC5077703.0301858476589385No Hit
TGAAAATCTTTGCTCTGAAGTGGAA4150912.4771114356708677No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT2499831.4918072134141924No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCT2008651.1986889345373155No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCT2000481.1938133770259671No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCT1753061.046162160446064No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGT1512970.9028852200666728No Hit
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGT1280560.7641914231006421No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACT1226120.7317036200507273No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGC1105110.6594892731170352No Hit
CTTCGGTACGCCCCTCCCCGCCTTCATTGACCTCATT667270.39820235747826377No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACT640500.38222699951268296No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTT620180.3701007658981666No Hit
GTTCAAGATAGTCGAACTCGACTCTGTT588430.3511535258754848No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC580970.3467016704244862No Hit
AGCTGTCGACTCCTGCACCAAGAAGAGTGT553860.3305234128807097No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAC549680.3280289415958338No Hit
AACTTTTGACGGTGGATCTCTTGGCTCACGTATCGATGAAGAACGCAGCC531160.31697688221882386No Hit
GCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGT506350.30217118064519444No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGC484540.2891557694674089No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACG438960.2619552907198865No Hit
GAGGTCCGGACGGAGGAGGGTTATCCAC435420.2598427480527907No Hit
TCAGGTCTAGGCTGGTTAGTTT425660.25401833663164497No Hit
CGACTTGAGCCCCTGACGAAGTAGGCT423760.2528844860476105No Hit
GGGGACTAGTTGGGAAGTGCTTAGGGTACTGTCGGCT381080.2274146213494039No Hit
TCCCGTAGATCCGAACTTGT380130.22684769605738667No Hit
GTTCAAGATAGTCGAACTCGACTCTGT372430.22225261737472057No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGT369970.2207845792501285No Hit
TGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGT359170.21433953382509033No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGCG354490.21154668080757372No Hit
GGTGACCGCCTGGGAATACCCCGTGTTGTAGGCTT347490.20736933655060452No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCC322240.1923010590522513No Hit
GCCCGGTTAGCTCAGTCGGTAGAGCATCAGACT306550.18293784028198745No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC303740.18126093494454695No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT300550.17935725949029957No Hit
TGAAAATCTTTTCTCTGAAGTGGAA300080.17907678066161734No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCC298670.1782353441755707No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCC291080.17370590947408548No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT276100.16476639276417138No Hit
CACCCCTTCTCCAAATACTAGACCTCTT266320.1589300460737201No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACGATTCT264320.15773651914315748No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT261600.1561133225175923No Hit
CACCCCTCTTCCACTAACTTGACCTCAT256120.15284305872785067No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCT232360.1386639587927666No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACGATT230370.13747639949685678No Hit
GAGGTCCGGATTTGGGGAGGGTTATCTACT229420.13690947420483954No Hit
GATTCTATAGACACCCCTTCTCCAAATACTAGACCTCTTT220250.13143715322820987No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA212190.12662723969804246No Hit
CACCCCTCTTCCACTAACTTGACCTCATT208380.12435357089532065No Hit
TGGGTTGAGTGAATTT205520.12264682738461609No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGC188630.11256749245601465No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCTGTGTTT186190.11111138960072824No Hit
AGCTGTCGACTCCTGCACCAAGAAGAGT184600.11016253569093096No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTACT176130.1051079491399982No Hit
GCCTACGACCATACCACAGGGAGAACACCGGTT173680.10364587865005898No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA169910.10139608038594841No Hit
TGGGAATACCCCGTGTTGTAGGCTT168670.10065609368899958No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)