FastQCFastQC Report
Mon 22 May 2023
sRNA-POR-73-S1-TP2_R2_001.fastp-trim.20230522.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POR-73-S1-TP2_R2_001.fastp-trim.20230522.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16390485
Sequences flagged as poor quality0
Sequence length15-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCA11643007.1035115800417135No Hit
ACCCGCTTCAAGTCTC3457942.1097240258601255No Hit
CCGCTTCAAGTCTCAGCCCGAG2861191.7456408397921113No Hit
ACCCGCTTCAAGTCTCAGCCCGAG2819901.7204493948775768No Hit
ACCCGCTTCAAGTCTCAGCCCGAGA2186141.333786035007506No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGC1745511.0649532335376288No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAA1704061.039664171011413No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAC1007480.6146736963549279No Hit
CAGCCCAGGCCGGCTCCAC983170.5998419204800833No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT922720.5629607665667001Illumina RNA PCR Primer (100% over 50bp)
AGTAGATAACCCTCCCCAAGTCCGGACCTC758020.46247563754214716No Hit
CGCTTCAAGTCTCAGCCCGAG751560.4585343264705102No Hit
ACCCGCTTCAAGTCTCAGCCCG735490.44872985759725836No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA647370.394966957963721Illumina RNA PCR Primer (100% over 50bp)
TGTCTTTCACCTACAGCATTAATA635480.38771274919564613No Hit
AGCCCAGGCCGGCTCCAC582160.35518168010281576No Hit
CCGCTTCAAGTCTCAGCCCGAGA552730.3372261406541661No Hit
ACAAGTTCGGATCTACGGGA538740.3286907007327727No Hit
GTGGATAACCCTCCTCCGTCCGGACCTC508850.31045451065053903No Hit
CAGCCCAGGCCGGCTCC481450.2937374946500973No Hit
CGGGAACAAGCCGAAACATTG459390.2802784664395227No Hit
CACCCGCTTCAAGTCTCAGCCCGAG423030.2580948641849219No Hit
AATTCAGAAAAACTGAACAGTCATCAGTGCT383710.23410533611421505No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGC372600.227327013203087No Hit
ATGAGGTCAAGTTAGTGGAAGAGGGGTG371520.22666809432423748No Hit
GCCCGCTTCAAGTCTC356530.21752254432983528No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGC342690.20907862091939317No Hit
AAACTAACCAGCCTAGACCTGA342610.20902981211355248No Hit
CGCTCTTCTAGGTGCAGGAGTCGACAGCT341070.20809024260111889No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA336290.2051739164521367Illumina RNA PCR Primer (100% over 50bp)
TCCACTTCAGAGCAAAGATTTTCA333560.20350831595282262No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAA332920.20311784550609696Illumina RNA PCR Primer (100% over 50bp)
AGGCGAGAATTCTACCACTGAACCACCAGTGC327720.19994527312645113No Hit
GTCTCATGCTCTACCGACTGAGCTAGCCGGGC308010.18792000358744723No Hit
TTTTTTTGGTCTTTCACCTACAGCATTAATA307970.18789559918452686No Hit
AATGAGGTCAAGTTAGT301210.18377125509098724No Hit
GCCCGCTTCAAGTCTCAGCCCGAG293560.17910391303246975No Hit
CACCCGCTTCAAGTCTC285400.17412541483671778No Hit
TAGTAGATAACCCTCCCCAAGTCCGGACCTC285360.17410101043379742No Hit
AAGGATGATAACCCTCGACCGTCCGGACCTC276760.16885406380592155No Hit
AATGAGGTCAAGTTAGTGGAAGAGGGGTG257350.1570118272888203No Hit
ACACTCTTCTTGGTGCAGGAGTCGACAGCT256560.15652984033114334No Hit
CCGCTTCAAGTCTCAGCCCG248180.15141711791932944No Hit
TCGCTTCAAGTCTCAGCCCGAG247390.15093513096165245No Hit
AGTAGTAGATAACCCTCCCCAAGTCCGGACCTC238480.14549905021114384No Hit
CCACTTCAGAGCAAAGATTTTCA233520.1424729042490201No Hit
AATGAGGTCAATGAAGGCGGGGAGGGGCGTACCGAAG230580.14067918063437415No Hit
GCCCGCTTCAAGTCTCAGCCCGAGA227830.1390013779335999No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAA225610.1376469335715203Illumina RNA PCR Primer (100% over 50bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGC221230.13497465145174167No Hit
ACCCGCTTCAAGTCTCA215740.13162514715092324No Hit
AAGAGGTCAAGTATTTGGAGGAGGGGTG209780.12798889111579065No Hit
ACACATCCACTAGCGGTAGGTTGCCAG206790.1261646619974943No Hit
GTAGTAGATAACCCTCCCCAAGTCCGGACCTC204190.12457837580767135No Hit
AAAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATAGAATC204180.12457227470694125No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCA202170.12334595346019352No Hit
TTTTTTTTTCCTTCACGAGTAAACATTCTA201560.12297378631565814No Hit
AATGAGGTCAAGTTAGTG197310.12038081850537065No Hit
GCTATTCAACCTCCTGACGATTTTAGGCA181800.11091801127300381No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAT178990.10920360196784903Illumina RNA PCR Primer (100% over 50bp)
GCTGAGTTCATCAACAGAATATTTAAACAA176820.10787966310941989No Hit
ACGGGATCGGTCACGGTAA175180.10687908258968541No Hit
GCTCTTCTAGGTGCAGGAGTCGACAGCT172350.10515247108307045No Hit
CCGCTTCAAGTCTCA168820.10299878252534932No Hit
CGCTTCAAGTCTCAGCCCGAGA168660.1029011649136679No Hit
TTTTTTGAGCCAAGACGCTATCACTA168130.10257780657497323No Hit
ACAGAGTCGAGTTCGACTATCTTGAACGGGCTGT167290.1020653141136458No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)