FastQCFastQC Report
Mon 22 May 2023
sRNA-POR-73-S1-TP2_R1_001.fastp-trim.20230522.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POR-73-S1-TP2_R1_001.fastp-trim.20230522.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16390485
Sequences flagged as poor quality0
Sequence length15-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA11126916.78863987246259No Hit
GAGACTTGAAGCGGGT3340092.037822553756036No Hit
CTCGGGCTGAGACTTGAAGCGGGT2729311.6651795233637074No Hit
CTCGGGCTGAGACTTGAAGCGG2694181.6437463564989079No Hit
TCTCGGGCTGAGACTTGAAGCGGGT2113441.2894310326997644No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC1761051.0744343440721857No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGT1684601.0277914289906613No Hit
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGT995540.6073889820832026No Hit
GTGGAGCCGGCCTGGGCTG928820.5666824380120539No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACT735390.4486688465899575No Hit
CGGGCTGAGACTTGAAGCGGGT712850.4349169655443387No Hit
CTCGGGCTGAGACTTGAAGCG708320.43215316691360867No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGCCGTCTTCTGCTT613180.3741072945675494TruSeq Adapter, Index 20 (98% over 58bp)
TATTAATGCTGTAGGTGAAAGACA601240.3668225802958241No Hit
GTGGAGCCGGCCTGGGCT562960.3434675667010464No Hit
TCTCGGGCTGAGACTTGAAGCGG522090.3185323680171758No Hit
TCCCGTAGATCCGAACTTGT519700.31707420494268473No Hit
GAGGTCCGGACGGAGGAGGGTTATCCAC490030.298972239076513No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCGCGTATGCCGTCTTCTGCTT467510.2852325602323543RNA PCR Primer, Index 20 (97% over 42bp)
GGAGCCGGCCTGGGCTG456180.27832001310516435No Hit
CAATGTTTCGGCTTGTTCCCG433420.2644339078434836No Hit
CTCGGGCTGAGACTTGAAGCGGGTG399690.24385489508089603No Hit
AGCACTGATGACTGTTCAGTTTTTCTGAATT384740.23473374948941414No Hit
GCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGT368740.224971988321273No Hit
CACCCCTCTTCCACTAACTTGACCTCAT367400.2241544408234412No Hit
GAGACTTGAAGCGGGC350500.2138435805895921No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT340800.20792551288140648No Hit
TCAGGTCTAGGCTGGTTAGTTT331330.20214777049001295No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCT326970.1994876905716945No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGCG322700.19688252055994684No Hit
TGAAAATCTTTGCTCTGAAGTGGA317710.1938380712956328No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA308980.1885118103582658No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAC306920.18725498360786763No Hit
ACTAACTTGACCTCATT293500.1790673064280892No Hit
CTCGGGCTGAGACTTGAAGCGGGC288200.17583372304114248No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTA284710.17370443888634168No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTT275920.16834157134459413No Hit
GAGACTTGAAGCGGGTG268170.16361321827877576No Hit
CACCCCTCTTCCACTAACTTGACCTCATT255010.15558416971797967No Hit
AGCTGTCGACTCCTGCACCAAGAAGAGTGT250530.15285087659090013No Hit
CTCGGGCTGAGACTTGAAGCGA239920.14637760871627656No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTACT237640.14498655774981645No Hit
CGGGCTGAGACTTGAAGCGG234880.1433026539483121No Hit
CTTCGGTACGCCCCTCCCCGCCTTCATTGACCTCATT229680.14013008156866621No Hit
TCTCGGGCTGAGACTTGAAGCGGGC224680.1370795312036221No Hit
TGAAAATCTTTGCTCTGAAGTGG220780.1347001019188877No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACT217880.1329307827071621No Hit
CACCCCTCCTCCAAATACTTGACCTCTT209680.12792788010848977No Hit
TGAGACTTGAAGCGGGT207370.12651852583983939No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTAC205620.12545083321207395No Hit
GATTCTATAGACACCCCTCCTCCAAATACTTGACCTCTTT205130.12515187927629964No Hit
CTGGCAACCTACCGCTAGTGGATGTGT200680.12243688945141039No Hit
TGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGT199940.12198540799738386No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAA196740.12003305576375563No Hit
CACTAACTTGACCTCATT192260.1172997626366761No Hit
TGCCTAAAATCGTCAGGAGGTTGAATAGC175150.10686077928749516No Hit
ACAGCCCGTTCAAGATAGTCGAACTCGACTCTGT169850.1036271959005484No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGC169640.10349907278521654No Hit
TTACCGTGACCGATCCCGT169260.10326723095747319No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGC167510.10219953832970777No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGCCGTCTTCTGCTT164920.1006193532406149TruSeq Adapter, Index 20 (98% over 62bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)