FastQCFastQC Report
Mon 22 May 2023
sRNA-POR-82-S1-TP2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POR-82-S1-TP2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20675792
Sequences flagged as poor quality0
Sequence length150
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA9403354.5479999024946665Illumina RNA PCR Primer (100% over 45bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC4999762.4181709701858094No Hit
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA3794241.8351122897734704Illumina DpnII expression Sequencing Primer (100% over 25bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT3191911.5437909222534256Illumina RNA PCR Primer (100% over 50bp)
GAGGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG2820771.3642863112571455Illumina RNA PCR Primer (100% over 37bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC2542131.2295200106482016No Hit
AGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTAGAACTCTGA2521551.2195663411587814Illumina DpnII expression Sequencing Primer (100% over 24bp)
AGCAGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTAGAACTC1834180.8871147475269628Illumina DpnII expression Sequencing Primer (100% over 21bp)
AAGGATGATAACCCTCGACCGTCCGGACCTCGATCGTCGGACTGTAGAAC1197300.5790830165054862No Hit
AGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT1096680.5304174079522564No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT1025630.49605354900068643No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC968280.46831579656053807No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA921810.4458402367367596No Hit
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT852480.4123082685296892No Hit
AAACTAACCAGCCTAGACCTGAGATCGTCGGACTGTAGAACTCTGAACGT757920.36657362387859194Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT751350.3633959946975671Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCCGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG684820.3312182672373566No Hit
AAGGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG661620.31999741533480314Illumina RNA PCR Primer (100% over 37bp)
GTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAGAACTCT657550.31802892967776036Illumina DpnII expression Sequencing Primer (100% over 22bp)
AATGAGGTCAATGAAGGCGGGGAGGGGCGTACCGAAGGATCGTCGGACTG537960.2601883400645547No Hit
AGAATCGTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAG536570.2595160562652207No Hit
AAGCCTACAACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA476350.23039020705954094No Hit
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT433400.20961712131752921No Hit
ACGGGATCGGTCACGGTAAGATCGTCGGACTGTAGAACTCTGAACGTGTA413310.19990044395880943Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACACATCCACTAGCGGTAGGTTGCCAGGATCGTCGGACTGTAGAACTCTG408520.1975837249668598Illumina DpnII expression Sequencing Primer (100% over 23bp)
ACACTCTTCTTGGTGCAGGAGTCGACAGCTGATCGTCGGACTGTAGAACT406800.19675183422236014No Hit
GGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTAGAACTCTGA379930.183755959626601Illumina DpnII expression Sequencing Primer (100% over 24bp)
AAATTCACTCAACCCAGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT377770.1827112596218805Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCTCTTCTAGGTGCAGGAGTCGACAGCTGATCGTCGGACTGTAGAACTCT376490.1820921781376017Illumina DpnII expression Sequencing Primer (100% over 22bp)
AATCGTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAGAA374780.18126512396719796No Hit
AAACACAGCAGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTA368370.17816487997170796No Hit
GCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACT364450.17626894292610412No Hit
TTCCACTTCAGAGAAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA362470.17531130125511032Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGCCGACAGTACCCTAAGCACTTCCCAACTAGTCCCCGATCGTCGGACTG343060.16592351093491364No Hit
ATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAGAACTCTGAAC337370.1631715002743305Illumina DpnII expression Sequencing Primer (100% over 26bp)
AAAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATAGAATCGATCGTCGGA336210.16261045767920282No Hit
AGTCCGGACCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG329020.1591329609042304Illumina RNA PCR Primer (100% over 33bp)
AGTAGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGA318010.15380789282461343No Hit
GGCAGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTAGAACTC316020.15284541457952372Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGGATGATAACCCTCGACCGTCCGGACCTCGATCGTCGGACTGTAGAACT289720.14012522470723252No Hit
GAATCGTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAGA288030.13930784368502064No Hit
AGCAGAGATTGGTGAGCTGAGAGATCGTCGGACTGTAGAACTCTGAACGT281260.13603348302207724Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTAGATAACCCTCCCCAAATCCGGACCTCGATCGTCGGACTGTAGAACT267650.1294509056775189No Hit
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA245640.11880560609238087No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCGATCGTCGG244800.11839933386832292No Hit
GCTGAGTTCATCAACAGAATATTTAAACAAGATCGTCGGACTGTAGAACT242970.11751424080876804No Hit
ATGAGGTCAAGTTAGTGGAAGAGGGGTGGATCGTCGGACTGTAGAACTCT240170.11616000006190814Illumina DpnII expression Sequencing Primer (100% over 22bp)
GATAACCCTCAACCGTCCGGACCTCGATCGTCGGACTGTAGAACTCTGAA232020.11221819217372665Illumina DpnII expression Sequencing Primer (100% over 25bp)
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC213430.10322700092939609No Hit
GTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAA210840.10197432823855067No Hit
AAGAATGGCGCAAGAATCCAAAGTGGTCAGATCGTCGGACTGTAGAACTC208250.10072165554770526Illumina DpnII expression Sequencing Primer (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)