FastQCFastQC Report
Mon 22 May 2023
sRNA-POR-82-S1-TP2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POR-82-S1-TP2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20675792
Sequences flagged as poor quality0
Sequence length150
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT5013402.4247680572526558No Hit
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC3657761.7691027265122419Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGAT2742201.3262853485854376TruSeq Adapter, Index 7 (97% over 36bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT2567641.2418581111669145No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTAGATCGGAAGAGCACACGTCTGAA2503731.2109475661198372Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATG2382691.152405673262722TruSeq Adapter, Index 7 (97% over 36bp)
GTGTGCTTAAGCGTGAGTAGTTGTCTGCTAGATCGGAAGAGCACACGTCT1827960.8841063984392956Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCGCGTATG1652360.799176157314796TruSeq Adapter, Index 7 (97% over 36bp)
GAGGTCCGGACGGTCGAGGGTTATCATCCTTAGATCGGAAGAGCACACGT1199370.5800841873433433No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTAGATCGGAAGAGCACACGTC1091550.5279362454410453No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTTTG1069940.5174844088197443TruSeq Adapter, Index 7 (97% over 36bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG1020560.49360140593405083No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG963140.46582979747523096No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG924650.4472138237800032No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC847050.4096820087956002No Hit
AACTTTTGACGGTGGATCTCTTGGCTCACGTATCGATGAAGAACGCAGCC760360.3677537479579984No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCGCGTTTG747380.3614758747814836TruSeq Adapter, Index 7 (97% over 36bp)
TCAGGTCTAGGCTGGTTAGTTTAGATCGGAAGAGCACACGTCTGAACTCC745320.3604795405177224Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG743780.3597347081069494Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGAT655790.31717769263687695TruSeq Adapter, Index 7 (97% over 36bp)
GAGGTCCGGACGGAGGAGGGTTATCCACAGATCGGAAGAGCACACGTCTG651710.31520437040573823Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGGTTG614860.29738159486224275TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCGCGGTTG548940.26549889842188396TruSeq Adapter, Index 7 (97% over 36bp)
GAGGTCCGGACGGAGGAGGGTTATCCACGATTCTAGATCGGAAGAGCACA539670.26101539423495845No Hit
CTTCGGTACGCCCCTCCCCGCCTTCATTGACCTCATTAGATCGGAAGAGC531170.2569043062534194No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGGATG516890.24999767844443394TruSeq Adapter, Index 7 (97% over 36bp)
GGTGACCGCCTGGGAATACCCCGTGTTGTAGGCTTAGATCGGAAGAGCAC476910.23066105520891295No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCGCGGATG473190.2288618496452276TruSeq Adapter, Index 7 (97% over 36bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC436380.21105842039811584No Hit
TTACCGTGACCGATCCCGTAGATCGGAAGAGCACACGTCTGAACTCCAGT410550.19856554950833322Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCTGTCGACTCCTGCACCAAGAAGAGTGTAGATCGGAAGAGCACACGTC409530.19807221895054855No Hit
CTGGCAACCTACCGCTAGTGGATGTGTAGATCGGAAGAGCACACGTCTGA408290.19747248376265342Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGTGCTTAAGCGTGAGTAGTTGTCCAGATCGGAAGAGCACACGTCTGAA389740.1885006388147066Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGGTCCGGACGGAGGAGGGTTATCCACGATTAGATCGGAAGAGCACACG377300.18248394063937187No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGCAGATCGGAAGAGCACACGTCTG375070.18140538461597988Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGTTGAGTGAATTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC371820.1798334980348032Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTGTGCTTAAGCGTGAGTAGTTGTCTGCTGTGTTTAGATCGGAAGAGCAC366760.17738619154226354No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC360020.17412634060160792No Hit
TGAAAATCTTTTCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC351480.1699959063236852Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGCCGTC350660.1695993072478191RNA PCR Primer, Index 23 (100% over 34bp)
GGGGACTAGTTGGGAAGTGCTTAGGGTACTGTCGGCTAGATCGGAAGAGC340980.16491750352296058No Hit
GAGGTCCGGACGGAGGAGGGTTATAGATCGGAAGAGCACACGTCTGAACT338880.16390182296281564Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GATTCTATAGACACCCCTCCTCCAAATACTTGACCTCTTTAGATCGGAAG337260.16311829795927527No Hit
GAGGTCCGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGT326780.1580495683067425TruSeq Adapter, Index 7 (97% over 36bp)
GTGTGCTTAAGCGTGAGTAGTTGTCTGCCAGATCGGAAGAGCACACGTCT324020.1567146738562663Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGGTCCGGACTTGGGGAGGGTTATCTACTACTAGATCGGAAGAGCACAC316990.15331456226682877No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCGCGTATGCCGTC304950.14749132705533116RNA PCR Primer, Index 23 (97% over 34bp)
GAGGTCCGGACGGTCGAGGGTTATCATCCTAGATCGGAAGAGCACACGTC289400.1399704543361628No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACGATTCAGATCGGAAGAGCACAC287260.13893542747963414No Hit
TCTCAGCTCACCAATCTCTGCTAGATCGGAAGAGCACACGTCTGAACTCC280100.13547244042694956Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAGGTCCGGATTTGGGGAGGGTTATCTACTAGATCGGAAGAGCACACGTC266810.12904463345346096No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC251080.12143670240056584No Hit
CACCCCTCTTCCACTAACTTGACCTCATAGATCGGAAGAGCACACGTCTG245960.11896037646345059Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGAA243760.11789633016234637No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGCAGATCGGAAGAGCACACGTC238860.11552640885534154No Hit
GAGGTCCGGACGGTTGAGGGTTATCAGATCGGAAGAGCACACGTCTGAAC226770.10967899077336433Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG213700.10335758842998613No Hit
TGACCACTTTGGATTCTTGCGCCATTCTTAGATCGGAAGAGCACACGTCT208060.10062976063988263Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT207870.10053786573205997No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)