FastQCFastQC Report
Mon 22 May 2023
sRNA-POR-79-S1-TP2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POR-79-S1-TP2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19762559
Sequences flagged as poor quality0
Sequence length150
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA13399696.78034155394552Illumina RNA PCR Primer (100% over 45bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC4813062.4354437094912655No Hit
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA4198612.1245274966668033Illumina DpnII expression Sequencing Primer (100% over 25bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT2548281.2894483958276861Illumina RNA PCR Primer (100% over 50bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC2404371.2166288788815254No Hit
AGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTAGAACTCTGA1985701.0047787839621376Illumina DpnII expression Sequencing Primer (100% over 24bp)
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA1935270.979260833579295No Hit
GAGGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG1921480.97228299229872Illumina RNA PCR Primer (100% over 37bp)
AGCAGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTAGAACTC1747960.884480597882086Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC1504800.7614398519948757No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT1278000.6466773862635906No Hit
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA1157270.5855871195628056No Hit
GCTGAGTTCATCAACAGAATATTTAAACAAGATCGTCGGACTGTAGAACT1116750.5650837019639006No Hit
AATGAGGTCAATGAAGGCGGGGAGGGGCGTACCGAAGGATCGTCGGACTG660140.3340356883944028No Hit
AGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT639130.3234044740865796No Hit
AAGGATGATAACCCTCGACCGTCCGGACCTCGATCGTCGGACTGTAGAAC599490.3033463429508294No Hit
AACAGAGTCGAGTTCGACTATCTTGAACGATCGTCGGACTGTAGAACTCT580790.2938840056087878Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT579500.29323125613439033No Hit
ACACTCTTCTTGGTGCAGGAGTCGACAGCTGATCGTCGGACTGTAGAACT547900.2772414240483735No Hit
TCCGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG515810.26100364836355455No Hit
GTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAA504870.25546792801478796No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCGATCGTCGG502330.2541826693597727No Hit
GCTCTTCTAGGTGCAGGAGTCGACAGCTGATCGTCGGACTGTAGAACTCT480050.24290882572444186Illumina DpnII expression Sequencing Primer (100% over 22bp)
AATCCGGACCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG469250.23744394640390448Illumina RNA PCR Primer (100% over 33bp)
AAGGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG454820.23014226042285313Illumina RNA PCR Primer (100% over 37bp)
CGTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAGAACTC449410.2274047606891395Illumina DpnII expression Sequencing Primer (100% over 21bp)
GTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAGAACTCT432890.2190455193580953Illumina DpnII expression Sequencing Primer (100% over 22bp)
AAACTAACCAGCCTAGACCTGAGATCGTCGGACTGTAGAACTCTGAACGT423540.21431435068707447Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCCTACTTCGTCAGGGGCTCAAGTCGGATCGTCGGACTGTAGAACTCTG417990.21150600992513166Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGTCCGGACCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG388940.1968064965675751Illumina RNA PCR Primer (100% over 33bp)
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT375390.18995009704967863Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCCGACAGTACCCTAAGCACTTCCCAACTAGTCCCCGATCGTCGGACTG373850.18917084573915757No Hit
ACTGAGTTCATCAACAGAATATTTAAACAAGATCGTCGGACTGTAGAACT373400.18894314243413518No Hit
ACAGAGTCGAGTTCGACTATCTTGAACGATCGTCGGACTGTAGAACTCTG365520.18495580455952088Illumina DpnII expression Sequencing Primer (100% over 23bp)
CGCTCTTCTAGGTGCAGGAGTCGACAGCTGATCGTCGGACTGTAGAACTC363590.18397921038464704Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAGATCGTCG356060.1801689750806057No Hit
AAGCCTACAACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA338690.1713796275067414No Hit
GGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA306090.15488378807623043No Hit
GCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACT303150.15339612648341744No Hit
TTCCACTTCAGAGAAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA302750.15319372354561978Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGTCTGATGCTCTACCGACTGAGCTAACCGGGCGATCGTCGGACTGTAGA296910.15023864065377363No Hit
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT296560.15006153808320066No Hit
GGCAGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTAGAACTC291830.1476681233437431Illumina DpnII expression Sequencing Primer (100% over 21bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGGA286760.1451026661071575Illumina RNA PCR Primer (97% over 45bp)
GGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTAGAACTCTGA283230.14331646018109295Illumina DpnII expression Sequencing Primer (100% over 24bp)
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG272600.13793760210911957Illumina DpnII expression Sequencing Primer (100% over 23bp)
AAGAGGTCTAGTATTTGGAGAAGGGGTGGATCGTCGGACTGTAGAACTCT258520.1308130186986412Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGAATCGTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAG255680.12937595784027767No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTC254270.12866248748454084Illumina RNA PCR Primer (97% over 44bp)
AGGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAAC252780.12790853654124448No Hit
ATGAGGTCAAGTTAGTGGAAGAGGGGTGGATCGTCGGACTGTAGAACTCT246860.12491297306183881Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGGATGATAACCCTCGACCGTCCGGACCTCGATCGTCGGACTGTAGAACT230920.1168472159906012No Hit
AGTAGATAACCCTCCCCAAATCCGGACCTCGATCGTCGGACTGTAGAACT228310.11552653682147135No Hit
AATCGTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAGAA222540.1126068744437398No Hit
AAAGAGGTCTAGTATTTGGAGAAGGGGTGTCTATAGAATCGATCGTCGGA216080.10933806699830727No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC207290.10489026244020322No Hit
AAATTCACTCAACCCAGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT204440.10344814150839474Illumina DpnII expression Sequencing Primer (96% over 30bp)
AATGAGGTCAAGTTAGTGGAAGAGGGGTGGATCGTCGGACTGTAGAACTC201130.10177325719811893Illumina DpnII expression Sequencing Primer (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)