FastQCFastQC Report
Mon 22 May 2023
sRNA-POR-73-S1-TP2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POR-73-S1-TP2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22960890
Sequences flagged as poor quality0
Sequence length150
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA365543315.920258317512953Illumina RNA PCR Primer (100% over 45bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA11440304.982515921638926Illumina DpnII expression Sequencing Primer (100% over 25bp)
CCGCTTCAAGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT4974792.1666363978051373Illumina RNA PCR Primer (100% over 31bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT4102001.7865161150112212Illumina RNA PCR Primer (100% over 50bp)
GAGGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG3436551.4966972099078042Illumina RNA PCR Primer (100% over 37bp)
ACCCGCTTCAAGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT3369801.4676260371440306Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGT2800731.21978285684919Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACCCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC2763821.203707695999589Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACCCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAA2149070.9359698165010154Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC1692530.7371360604924286No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1686220.7343879091794786No Hit
CGCTTCAAGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC1012760.4410804633444087Illumina RNA PCR Primer (100% over 32bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT1003130.4368863750490508No Hit
CAGCCCAGGCCGGCTCCACGATCGTCGGACTGTAGAACTCTGAACGTGTA961040.41855520408834324Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGGA775330.3376741929428694Illumina RNA PCR Primer (97% over 45bp)
AGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT749970.3266293249085728No Hit
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG735090.3201487398789855Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACCCGCTTCAAGTCTCAGCCCGGATCGTCGGACTGTAGAACTCTGAACGT718210.3127971084744537Illumina DpnII expression Sequencing Primer (96% over 28bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTC709670.3090777404534406Illumina RNA PCR Primer (97% over 44bp)
TGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAACTCTGAAC624360.2719232573301819Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCCCAGGCCGGCTCCACGATCGTCGGACTGTAGAACTCTGAACGTGTAG568320.24751653790423628Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAACG542970.23647602510181442Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT528520.23018271504284027Illumina DpnII expression Sequencing Primer (96% over 30bp)
GTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAGAACTCT501260.21831035295234638Illumina DpnII expression Sequencing Primer (100% over 22bp)
CAGCCCAGGCCGGCTCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA470730.20501383003881818Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCTTCAAGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG467570.20363757676640581Illumina RNA PCR Primer (100% over 33bp)
CGGGAACAAGCCGAAACATTGGATCGTCGGACTGTAGAACTCTGAACGTG449410.19572847568190954Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCGCTTCAAGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT437740.19064592008410824Illumina RNA PCR Primer (100% over 31bp)
CACCCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAA414780.18064630769974507Illumina DpnII expression Sequencing Primer (100% over 25bp)
AATTCAGAAAAACTGAACAGTCATCAGTGCTGATCGTCGGACTGTAGAAC370890.1615311950015875No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCGATCGTCGG369230.1608082265103835No Hit
ATGAGGTCAAGTTAGTGGAAGAGGGGTGGATCGTCGGACTGTAGAACTCT361510.15744598750309766Illumina DpnII expression Sequencing Primer (100% over 22bp)
CCGCTTCAAGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG348180.1516404634140924Illumina RNA PCR Primer (100% over 33bp)
GCCCGCTTCAAGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT346530.15092185015476317Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAACTAACCAGCCTAGACCTGAGATCGTCGGACTGTAGAACTCTGAACGT336780.1466754990769086Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCTCTTCTAGGTGCAGGAGTCGACAGCTGATCGTCGGACTGTAGAACTC336490.14654919735254165Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC330960.14414075412582003No Hit
AGGCCGGCTCCACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC329510.14350924550398525Illumina RNA PCR Primer (100% over 32bp)
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC328170.14292564443277242Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACCCGCTTCAAGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT320880.1397506803960996Illumina RNA PCR Primer (100% over 31bp)
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA317830.13842233467430923No Hit
TTTTTTTGGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAAC299530.13045226034356683No Hit
AATGAGGTCAAGTTAGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA293550.1278478316824827Illumina DpnII expression Sequencing Primer (96% over 30bp)
GTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAA288660.12571812329574333No Hit
GCCCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC287520.12522162686202495Illumina DpnII expression Sequencing Primer (100% over 26bp)
AAGGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG283980.12367987477837313Illumina RNA PCR Primer (100% over 37bp)
CACCCGCTTCAAGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA277970.12106238042166484Illumina DpnII expression Sequencing Primer (96% over 30bp)
TAGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAAC277060.12066605432106507No Hit
AAGGATGATAACCCTCGACCGTCCGGACCTCGATCGTCGGACTGTAGAAC269070.1171862240531617No Hit
CATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA268270.1168378055031839Illumina RNA PCR Primer (100% over 45bp)
ACACTCTTCTTGGTGCAGGAGTCGACAGCTGATCGTCGGACTGTAGAACT252750.11007848563361437No Hit
AATGAGGTCAAGTTAGTGGAAGAGGGGTGGATCGTCGGACTGTAGAACTC250520.10910726892555123Illumina DpnII expression Sequencing Primer (100% over 21bp)
CCGCTTCAAGTCTCAGCCCGGATCGTCGGACTGTAGAACTCTGAACGTGT242230.10549678170140618Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGT242220.10549242646953146Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTAGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGA232680.10133753526104608No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)