FastQCFastQC Report
Mon 22 May 2023
sRNA-ACR-178-S1-TP2_R2_001.fastp-trim.20230522.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-178-S1-TP2_R2_001.fastp-trim.20230522.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16727098
Sequences flagged as poor quality0
Sequence length15-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGAGAATTCTACCACTGAACCACCAGTGC5474993.2731260377621987No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGC3947532.359961064375901No Hit
GGCGAGAATTCTACCACTGAACCACAGTGC2111071.2620659004927215No Hit
TTCCACCTCAGAGCAAAGATTTTCA2012181.2029462612104025No Hit
AGCGAGAATTCTACCACTGAACCACAGTGC1594640.9533273494302479No Hit
GCTTGTCAGTGGTAACTCAGGTCTTGGA1546780.924715094034841No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGC1434370.8575127616278688No Hit
ACTTGGTAGACAGGTTCCGCACATAAAAGA1422770.8505779065800894No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT1406200.8406718248437356Illumina RNA PCR Primer (100% over 50bp)
ACAAGTTCGGATCTACGGGA1257430.7517323088559653No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA1099170.6571193640403136Illumina RNA PCR Primer (100% over 50bp)
ACTTGTCAGTGGTAACTCAGGTCTTGGA846120.5058378925023336No Hit
CCACAAGTTCGGATCTACGGGA630610.37699904669656387No Hit
TGCGAGAATTCTACCACTGAACCACAGTGC592820.3544069628814275No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGC589180.35223085319402087No Hit
GAGTGGATAACCCTCCCCAAGTCCGGACCTC573160.34265357924010487No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA568850.3400769218904558Illumina RNA PCR Primer (100% over 50bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAA542910.32456915120602514Illumina RNA PCR Primer (100% over 50bp)
CCAGGTCAACAGTCCGGAAATCATGTCAA454030.27143381356407426No Hit
GCTCGTCAGTGGTAACTCAGGTCTTGGA429090.2565238752113487No Hit
ACTGCTTGTCAGTGGTAACTCAGGTCTTGGA353970.2116147104536603No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAT300990.1799415535199232Illumina RNA PCR Primer (100% over 50bp)
ACTCTTCATTTATCTACTCGTTAA293110.17523063474608686No Hit
GCAAGTTCGGATCTACGGGA278090.16625119312387598No Hit
ACTCGTCAGTGGTAACTCAGGTCTTGGA272760.16306474679588773No Hit
AGTGGATAACCCTCCCCAAGTCCGGACCTC270240.16155820931999082No Hit
GCTTGGTAGACAGGTTCCGCACATAAAAGA266750.15947177448233996No Hit
AAGCACAAAGAACTCAAAGAAGGAAGTA262160.15672772407981347No Hit
CTGCTTGTCAGTGGTAACTCAGGTCTTGGA252140.15073744411612822No Hit
AGTGAATCCATGTACGATTTTCGATGTGA241730.14451400954307794No Hit
AGGGACGCTCGGACAGCCTTGCTCGCCTCGCAAGAGACGAGCGCCATTTG233570.1396356977163642No Hit
GAGAATTCTACCACTGAACCACCAGTGC232300.13887645065509868No Hit
AGGCGAGAATTCTACCACTGAACCACAGTGC231670.13849981628612446No Hit
GGCAGTCTGCTCAGAATTTCCACCAGAA231080.13814709521041843No Hit
TCACAAGTTCGGATCTACGGGA220750.1319714872239046No Hit
AATCATACCACTCTTAGCCCGTCATTTAA219730.13136169824556537No Hit
AGCAGTCTGCTCAGAATTTCCACCAGAA216320.1293230899944509No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAC214190.12804970712791902Illumina RNA PCR Primer (100% over 50bp)
AGAGTGGATAACCCTCCCCAAGTCCGGACCTC211660.12653719132870508No Hit
ACACGCTTTGTCCATAACAAGAGACCATA204780.12242410488657388No Hit
GCGAGAATTCTACCACTGAACCACCAGTGC198300.11855015137712471No Hit
AGCCGGAGATCCTAACCACCTAGACTACGTTGGA194190.11609306049381668No Hit
ACTTTTTATTTACGGAATCCACAATCTTA191820.11467619786767555No Hit
ATTGTAAGCTTATATATACGAGTTAGATCA188810.11287672254924315No Hit
AGTTTTGGCTTAACAAGTCTGGAGGCTCTA186930.11175279776563753No Hit
GAATGCTCTACCACTGAGCTACACCCCC173640.10380760607727653No Hit
AAGAGTGGATAACCCTCCCCAAGTCCGGACCTC169590.10138638513387078No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)