FastQCFastQC Report
Mon 22 May 2023
sRNA-ACR-178-S1-TP2_R1_001.fastp-trim.20230522.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-178-S1-TP2_R1_001.fastp-trim.20230522.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16727098
Sequences flagged as poor quality0
Sequence length15-150
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC5543483.3140715741606828No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT3936442.353331103817291No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC2174041.2997114024201928No Hit
TGAAAATCTTTGCTCTGAGGTGGAA2061831.2326286364795613No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT1624400.9711188396217922No Hit
TCCAAGACCTGAGTTACCACTGACAAGC1595820.9540327915816599No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGT1454540.8695710397583609No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA1427940.8536686997350048No Hit
TCCCGTAGATCCGAACTTGT1281940.7663851793060578No Hit
TCCAAGACCTGAGTTACCACTGACAAGT863090.5159831071713695No Hit
TCCCGTAGATCCGAACTTGTGG641780.38367683384171003No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA604570.36143149277896264No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCT589180.35223085319402087No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC577560.3452840414996074No Hit
TTGACATGATTTCCGGACTGTTGACCTGG466120.27866160645438914No Hit
TCCAAGACCTGAGTTACCACTGACGAGC444080.26548538186360837No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT353190.21114840123493028No Hit
TTAACGAGTAGATAAATGAAGAGT298880.1786801273000254No Hit
TCCCGTAGATCCGAACTTGC288130.17225342973419538No Hit
TCCAAGACCTGAGTTACCACTGACGAGT278560.1665321743197774No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT276670.16540227121285472No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGC276360.16521694319002614No Hit
TACTTCCTTCTTTGAGTTCTTTGTGCTT268230.16035656633326356No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG257700.15406139188040865No Hit
TCACATCGAAAATCGTACATGGATTCACT249490.14915318843710965No Hit
GAGACTCTGCACGGTGGATCTCTCGGCTCGCGCATCGATGAAGAACGCAG246110.14713251515594636No Hit
TTCTGGTGGAAATTCTGAGCAGACTGCC239610.1432466049998631No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC238570.14262485937488978No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT231070.1381411168871014No Hit
TCCCGTAGATCCGAACTTGTGA224980.13450031798701723No Hit
TTAAATGACGGGCTAAGAGTGGTATGATT224700.1343329249341398No Hit
TTCTGGTGGAAATTCTGAGCAGACTGCT221890.1326530160820484No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCT211690.12655512629865623No Hit
TATGGTCTCTTGTTATGGACAAAGCGTGT208590.12470184607037037No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC208080.12439695158120076No Hit
TAAGATTGTGGATTCCGTAAATAAAAAGT196240.11731861677381217No Hit
TGATCTAACTCGTATATATAAGCTTACAAT194850.11648762983274205No Hit
TCCAACGTAGTCTAGGTGGTTAGGATCTCCGGCT194100.11603925558396322No Hit
TAGAGCCTCCAGACTTGTTAAGCCAAAACT192020.11479576433401656No Hit
GGGGGTGTAGCTCAGTGGTAGAGCATTC178520.10672502785599751No Hit
TTGACATGATTTCCGGACTGTTGACCTGA171260.10238476512781836No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTT169040.10105757735143299No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)