FastQCFastQC Report
Mon 22 May 2023
sRNA-ACR-173-S1-TP2_R2_001.fastp-trim.20230522.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-173-S1-TP2_R2_001.fastp-trim.20230522.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18763199
Sequences flagged as poor quality0
Sequence length15-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGAGAATTCTACCACTGAACCACCAGTGC2843721.515583776519132No Hit
GCTTGTCAGTGGTAACTCAGGTCTTGGA2226861.18682320642658No Hit
AGAATTCTACCACTGAACCACCAGTGC2188661.1664642047446172No Hit
TTCCGCCTCAGAAGAAAGATTTTCA2186901.1655261983844014No Hit
AGAATCATACCACTAGACCAAACAGCC1798100.9583120660821218No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGC1371040.7307069546083267No Hit
ACTGCTTGTCAGTGGTAACTCAGGTCTTGGA1247910.665083816464346No Hit
GGCGAGAATTCTACCACTGAACCACAGTGC1202380.640818231475347No Hit
AGAATTCTACCACTGAACCACAGTGC1005440.5358574515998045No Hit
ACAAGTTCGGATCTACGGGA948340.5054255407087033No Hit
ACTTGGTAGACAGGTTCCGCACATAAAAGA928920.4950754932567736No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGC907350.48357958576253446No Hit
GAGAATTCTACCACTGAACCACCAGTGC877560.4677027621995588No Hit
TTCCGCCCCAGAAGAAAGATTTTCA802100.42748573950529434No Hit
ACTTGTCAGTGGTAACTCAGGTCTTGGA736130.39232648974196777No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT727150.3875405254722289Illumina RNA PCR Primer (100% over 50bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA682470.3637279549185616Illumina RNA PCR Primer (100% over 50bp)
TGCGAGAATTCTACCACTGAACCACCAGTGC676880.3607487188085571No Hit
CCACAAGTTCGGATCTACGGGA636030.3389773780046782No Hit
GAGTGGATAACCCTCCCCAAGTCCGGACCTC623290.3321874910562959No Hit
AGCGAGAATTCTACCACTGAACCACAGTGC599800.31966830389636647No Hit
GCTCGTCAGTGGTAACTCAGGTCTTGGA595200.3172166963639836No Hit
TTCCGTCTCAGAAGAAAGATTTTCA522520.2784812973523332No Hit
AGTGAATCCATGTACGATTTTCGATGTGA518960.27658396630553245No Hit
GGAGTCTCCAGATCAGGTCATTGTTACTA504660.26896266462877677No Hit
CTGCTTGTCAGTGGTAACTCAGGTCTTGGA488360.2602754466335938No Hit
AGGGACGCTCGGACAGCCTTGCTCGCCTCGCAAGAGACGAGCGCCATTTG484900.2584314114027144No Hit
CCAGGTCAACAGTCCGGAAATCATGTCAA425210.22661913887924975No Hit
GAGAATTCTACCACTGAACCACAGTGC400290.2133378215516448No Hit
AGGCGAGAATTCTACCACTGAACCACAGTGC395390.21072632657149776No Hit
GGAATTCTACCACTGAACCACCAGTGC380230.2026466808778183No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA367420.1958194868582911Illumina RNA PCR Primer (100% over 50bp)
AAGAGGTCAATGTGCGGGGAGGGGTG355900.18967980886414945No Hit
AGTGGATAACCCTCCCCAAGTCCGGACCTC340830.18164812940479924No Hit
GGAATCATACCACTAGACCAAACAGCC336530.17935640932018043No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAA332450.1771819400305886Illumina RNA PCR Primer (100% over 50bp)
GCCGGATCCTAGCCACTAGACCATATGGGA325730.17360046120067266No Hit
ACTTGGTCGAGAGGTTCCACTCATAAAAGA312720.16666667554930265No Hit
TGCGAGAATTCTACCACTGAACCACAGTGC299460.15959965035812923No Hit
TTTTTTTGGCCTTTAACCTACAGCATTAATA291080.1551334609839186No Hit
ACTGCTCGTCAGTGGTAACTCAGGTCTTGGA290140.15463248031425772No Hit
AGCCGAGATATCCGTCGCTGAAAGTTGTG272120.14502857428522717No Hit
GAGAATCATACCACTAGACCAAACAGCC266450.14200670152248557No Hit
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGC246470.1313581975013962No Hit
ATTTCAGATTGTGCCGAAAGTATGTTCGTA240570.12821374436203548No Hit
ACTCGTCAGTGGTAACTCAGGTCTTGGA240240.12803786816949497No Hit
TGCTTGTCAGTGGTAACTCAGGTCTTGGA235000.1252451674152153No Hit
TGGCGAGAATTCTACCACTGAACCACCAGTGC231990.12364096335598211No Hit
ACTGCTCGTCAGTAGTAACTCAGGCCTTGGA224660.11973438004894582No Hit
GCTGCTTGTCAGTGGTAACTCAGGTCTTGGA223970.11936663891908836No Hit
GGGCGAGAATTCTACCACTGAACCACCAGTGC220370.11744798954591912No Hit
AGTTTTGGCTTAACAAGTCTGGAGGCTCTA213120.11358404289161991No Hit
TTTCATTTGTATCCTCACGGTTGACCCCT206590.11010382611195459No Hit
ATTGTAAGCTTATATATACGAGTTAGATCA204700.10909653519104072No Hit
CGGATCCTAGCCACTAGACCATATGGGA202060.1076895256507166No Hit
TGTCAGTGGTAACTCAGGTCTTGGA199510.1063304823447217No Hit
GCAAGTTCGGATCTACGGGA199340.10623987945765538No Hit
TCACAAGTTCGGATCTACGGGA199040.10607999200989127No Hit
AGAGTCTCCAGATCAGGTCATTGTTACTA195220.104044091841695No Hit
AGGCTGATCCGAGGTCCG194220.10351113368248133No Hit
ACTTGGTTGACAGGTTCCGCACATAAAAGA194100.10344717870337569No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)