FastQCFastQC Report
Mon 22 May 2023
sRNA-ACR-173-S1-TP2_R1_001.fastp-trim.20230522.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-173-S1-TP2_R1_001.fastp-trim.20230522.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18763199
Sequences flagged as poor quality0
Sequence length15-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC2877331.5334965002503038No Hit
TCCAAGACCTGAGTTACCACTGACAAGC2286091.2183903181968065No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT2236021.1917051031649772No Hit
TGAAAATCTTTCTTCTGAGGCGGAA2233081.190138206176889No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT1834960.977956903830738No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT1368610.7294118662814374No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT1245040.6635542265474027No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1237760.6596742911483271No Hit
GCACTGTGGTTCAGTGGTAGAATTCT1026630.5471508349935424No Hit
TCCCGTAGATCCGAACTTGT966190.5149388438506675No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGT948940.5057453156042314No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCT908070.4839633156371682No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC900690.48003008442217127No Hit
TGAAAATCTTTCTTCTGGGGCGGAA818590.436274219550728No Hit
TCCAAGACCTGAGTTACCACTGACAAGT749370.3993828557699569No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA674520.35949093755281286No Hit
TCCCGTAGATCCGAACTTGTGG648600.3456766620659942No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC627770.3345751436095732No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT614140.3273109238994907No Hit
TCCAAGACCTGAGTTACCACTGACGAGC613780.3271190589621738No Hit
TGAAAATCTTTCTTCTGAGACGGAA533560.2843651554300522No Hit
TCACATCGAAAATCGTACATGGATTCACT532880.28400274388178687No Hit
TAGTAACAATGACCTGATCTGGAGACTCC519810.2770369807408641No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG496750.2647469655893966No Hit
GAGACTCTGCACGGTGGATCTCTCGGCTCGCGCATCGATGAAGAACGCAG467280.24904068863736933No Hit
TTGACATGATTTCCGGACTGTTGACCTGG435270.23198069796093937No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC409750.21837960573780624No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT395330.21069434908194493No Hit
GCACTGGTGGTTCAGTGGTAGAATTCC395330.21069434908194493No Hit
CACCCCTCCCCGCACATTGACCTCTT369090.1967095269841779No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT348010.18547476898795348No Hit
GGCTGTTTGGTCTAGTGGTATGATTCC347930.1854321323352164No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGC336660.1794256938808782No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGT319010.17001898237075672No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA306400.16329837998307217No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA291390.15529867801327482No Hit
TCCAAGACCTGAGTTACCACTGACGAGCAGT290710.1549362664650095No Hit
CACAACTTTCAGCGACGGATATCTCGGCT283350.15101369441319681No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTC272240.1450925292643328No Hit
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCA249090.13275454787853605No Hit
TACGAACATACTTTCGGCACAATCTGAAAT247890.13211499808747965No Hit
TCCAAGACCTGAGTTACCACTGACGAGT246000.13110770716656578No Hit
TCCAAGACCTGAGTTACCACTGACAAGCA243630.12984459632922937No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCA232530.12392876076195748No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGC225600.12023536071860669No Hit
TCCAAGGCCTGAGTTACTACTGACGAGCAGT224720.11976635753849862No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCC222650.11866313414892632No Hit
AGACTCTGCACGGTGGATCTCTCGGCTCGCGCATCGATGAAGAACGCAGC220830.11769315029915742No Hit
TAGAGCCTCCAGACTTGTTAAGCCAAAACT217910.11613691247425346No Hit
AGGGGTCAACCGTGAGGATACAAATGAAA214450.11429287724337411No Hit
TGATCTAACTCGTATATATAAGCTTACAAT210950.11242752368612623No Hit
TCCCATATGGTCTAGTGGCTAGGATCCG206820.11022640648857374No Hit
TCCCGTAGATCCGAACTTGC206060.10982135828757132No Hit
TCCAAGACCTGAGTTACCACTGACA204240.10885137443780242No Hit
TCCCGTAGATCCGAACTTGTGA203810.10862220242934054No Hit
TAGTAACAATGACCTGATCTGGAGACTCT200420.10681547426960615No Hit
TCTTTTATGTGCGGAACCTGTCAACCAAGT198980.10604801452033846No Hit
CGGACCTCGGATCAGCCT198500.1057921946039159No Hit
TCTTTTATGTGCAGAACCTGTCGACCAAGT191090.1018429746441425No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)